Uniprot ID	J3F2B0
Uniprot Name	CAS9_ACTNH
Taxonomy ID	1115803
Sequence	M W Y A S L M S A H H L R V G I D V G T H S V G L A T L R V D D H G T P I E L L S A L S H I H D S G V G K E G K K D H D T R K K L S G I A R R A R R L L H H R R T Q L Q Q L D E V L R D L G F P I P T P G E F L D L N E Q T D P Y R V W R V R A R L V E E K L P E E L R G P A I S M A V R H I A R H R G W R N P Y S K V E S L L S P A E E S P F M K A L R E R I L A T T G E V L D D G I T P G Q A M A Q V A L T H N I S M R G P E G I L G K L H Q S D N A N E I R K I C A R Q G V S P D V C K Q L L R A V F K A D S P R G S A V S R V A P D P L P G Q G S F R R A P K C D P E F Q R F R I I S I V A N L R I S E T K G E N R P L T A D E R R H V V T F L T E D S Q A D L T W V D V A E K L G V H R R D L R G T A V H T D D G E R S A A R P P I D A T D R I M R Q T K I S S L K T W W E E A D S E Q R G A M I R Y L Y E D P T D S E C A E I I A E L P E E D Q A K L D S L H L P A G R A A Y S R E S L T A L S D H M L A T T D D L H E A R K R L F G V D D S W A P P A E A I N A P V G N P S V D R T L K I V G R Y L S A V E S M W G T P E V I H V E H V R D G F T S E R M A D E R D K A N R R R Y N D N Q E A M K K I Q R D Y G K E G Y I S R G D I V R L D A L E L Q G C A C L Y C G T T I G Y H T C Q L D H I V P Q A G P G S N N R R G N L V A V C E R C N R S K S N T P F A V W A Q K C G I P H V G V K E A I G R V R G W R K Q T P N T S S E D L T R L K K E V I A R L R R T Q E D P E I D E R S M E S V A W M A N E L H H R I A A A Y P E T T V M V Y R G S I T A A A R K A A G I D S R I N L I G E K G R K D R I D R R H H A V D A S V V A L M E A S V A K T L A E R S S L R G E Q R L T G K E Q T W K Q Y T G S T V G A R E H F E M W R G H M L H L T E L F N E R L A E D K V Y V T Q N I R L R L S D G N A H T V N P S K L V S H R L G D G L T V Q Q I D R A C T P A L W C A L T R E K D F D E K N G L P A R E D R A I R V H G H E I K S S D Y I Q V F S K R K K T D S D R D E T P F G A I A V R G G F V E I G P S I H H A R I Y R V E G K K P V Y A M L R V F T H D L L S Q R H G D L F S A V I P P Q S I S M R C A E P K L R K A I T T G N A T Y L G W V V V G D E L E I N V D S F T K Y A I G R F L E D F P N T T R W R I C G Y D T N S K L T L K P I V L A A E G L E N P S S A V N E I V E L K G W R V A I N V L T K V H P T V V R R D A L G R P R Y S S R S N L P T S W T I E
Cofactor	COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:24505130}; Note=Binds 2 Mg(2+) per subunit. {ECO:0000269|PubMed:24505130}; COFACTOR: Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:24505130}; Note=Binds 2 Mn(2+) per subunit. {ECO:0000269|PubMed:24505130}; COFACTOR: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:24505130}; Note=Binds 1 Zn(2+) per subunit, which may stabilize the HNH Cas9 architecture. {ECO:0000269|PubMed:24505130};
Active site	ACT_SITE 17 17 For RuvC-like nuclease domain. {ECO:0000250|UniProtKB:Q99ZW2}.; ACT_SITE 582 582 Proton acceptor for HNH nuclease domain. {ECO:0000269|PubMed:24505130}.
Pfam	PF18470 PF17893 PF18525 PF17894 PF01844 PF18541
InterPro	IPR040619 IPR040796 IPR041217 IPR041225 IPR002711 IPR033114 IPR003615 IPR036397 IPR041383