Uniprot ID	P14294
Uniprot Name	TOP3_ECOLI
Taxonomy ID	83333
Sequence	M R L F I A E K P S L A R A I A D V L P K P H R K G D G F I E C G N G Q V V T W C I G H L L E Q A Q P D A Y D S R Y A R W N L A D L P I V P E K W Q L Q P R P S V T K Q L N V I K R F L H E A S E I V H A G D P D R E G Q L L V D E V L D Y L Q L A P E K R Q Q V Q R C L I N D L N P Q A V E R A I D R L R S N S E F V P L C V S A L A R A R A D W L Y G I N M T R A Y T I L G R N A G Y Q G V L S V G R V Q T P V L G L V V R R D E E I E N F V A K D F F E V K A H I V T P A D E R F T A I W Q P S E A C E P Y Q D E E G R L L H R P L A E H V V N R I S G Q P A I V T S Y N D K R E S E S A P L P F S L S A L Q I E A A K R F G L S A Q N V L D I C Q K L Y E T H K L I T Y P R S D C R Y L P E E H F A G R H A V M N A I S V H A P D L L P Q P V V D P D I R N R C W D D K K V D A H H A I I P T A R S S A I N L T E N E A K V Y N L I A R Q Y L M Q F C P D A V F R K C V I E L D I A K G K F V A K A R F L A E A G W R T L L G S K E R D E E N D G T P L P V V A K G D E L L C E K G E V V E R Q T Q P P R H F T D A T L L S A M T G I A R F V Q D K D L K K I L R A T D G L G T E A T R A G I I E L L F K R G F L T K K G R Y I H S T D A G K A L F H S L P E M A T R P D M T A H W E S V L T Q I S E K Q C R Y Q D F M Q P L V G T L Y Q L I D Q A K R T P V R Q F R G I V A P G S G G S A D K K K A A P R K R S A K K S P P A D E V G S G A I A
Cofactor	COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00953, ECO:0000269|PubMed:6326814}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_00953, ECO:0000269|PubMed:6326814}; Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000255|HAMAP-Rule:MF_00953, ECO:0000269|PubMed:6326814}; Note=Binds two Mg(2+) ions per subunit. The magnesium ions form salt bridges with both the protein and the DNA. Can also accept other divalent metal cations, such as Mn(2+) or Ca(2+). {ECO:0000255|HAMAP-Rule:MF_00953, ECO:0000269|PubMed:6326814};
Active site	ACT_SITE 328 328 O-(5'-phospho-DNA)-tyrosine intermediate. {ECO:0000255|HAMAP-Rule:MF_00953, ECO:0000269|PubMed:10574789}.
Pfam	PF01131 PF01751
InterPro	IPR000380 IPR003601 IPR023406 IPR013497 IPR013824 IPR013825 IPR013826 IPR023405 IPR003602 IPR005738 IPR006171 IPR034144