Uniprot ID	Q46896
Uniprot Name	CAS1_ECOLI
Taxonomy ID	83333
Sequence	M T W L P L N P I P L K D R V S M I F L Q Y G Q I D V I D G A F V L I D K T G I R T H I P V G S V A C I M L E P G T R V S H A A V R L A A Q V G T L L V W V G E A G V R V Y A S G Q P G G A R S D K L L Y Q A K L A L D E D L R L K V V R K M F E L R F G E P A P A R R S V E Q L R G I E G S R V R A T Y A L L A K Q Y G V T W N G R R Y D P K D W E K G D T I N Q C I S A A T S C L Y G V T E A A I L A A G Y A P A I G F V H T G K P L S F V Y D I A D I I K F D T V V P K A F E I A R R N P G E P D R E V R L A C R D I F R S S K T L A K L I P L I E D V L A A G E I Q P P A P P E D A Q P V A I P L P V S L G D A G H R S S
Cofactor	COFACTOR: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:21219465, ECO:0000269|PubMed:25707795, ECO:0000269|PubMed:26284603}; Note=Protospacer integration in vitro also occurs with Mn(2+) and also requires low concentrations of KCl (PubMed:25707795). The transesterification function works equally well with Mg(2+), Mn(2+) or Co(2+) in vitro (PubMed:26284603). {ECO:0000269|PubMed:25707795, ECO:0000269|PubMed:26284603};
Active site	ACT_SITE 141 141 {ECO:0000305|PubMed:26478180, ECO:0000305|PubMed:26503043}.; ACT_SITE 208 208 {ECO:0000305|PubMed:26478180, ECO:0000305|PubMed:26503043}.; ACT_SITE 221 221 {ECO:0000305|PubMed:26478180, ECO:0000305|PubMed:26503043}.
Pfam	PF01867
InterPro	IPR033641 IPR002729 IPR042206 IPR019851 IPR042211