Aspartate aminotransferase, cytoplasmic

NameAspartate aminotransferase, cytoplasmic
Synonyms2.6.1.1 cAspAT cCAT Cysteine aminotransferase, cytoplasmic Cysteine transaminase, cytoplasmic Glutamate oxaloacetate transaminase 1 Transaminase A
Gene NameGOT1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037039|Aspartate aminotransferase, cytoplasmic
MAPPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQK
IANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFL
ARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLEN
APEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWA
IRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPP
AQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGM
FSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ
Number of residues413
Molecular Weight46247.14
Theoretical pI7.01
GO Classification
Functions
    carboxylic acid binding
L-cysteine
pyridoxal phosphate binding
identical protein binding
L-aspartate
phosphatidylserine decarboxylase activity
L-phenylalanine
Processes
    small molecule metabolic process
aspartate catabolic process
Notch signaling pathway
2-oxoglutarate metabolic process
fatty acid homeostasis
carbohydrate metabolic process
oxaloacetate metabolic process
glycerol biosynthetic process
glucose metabolic process
glutamate metabolic process
L-methionine biosynthetic process from methylthioadenosine
gluconeogenesis
response to glucocorticoid
aspartate biosynthetic process
polyamine metabolic process
aspartate metabolic process
cellular amino acid biosynthetic process
cellular response to insulin stimulus
glutamate catabolic process to 2-oxoglutarate
cellular nitrogen compound metabolic process
sulfur amino acid metabolic process
glutamate catabolic process to aspartate
Components
    cytosol
extracellular exosome
cytoplasm
nucleus
axon terminus
lysosome
General FunctionPyridoxal phosphate binding
Specific FunctionBiosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
Transmembrane Regions
GenBank Protein ID179067
UniProtKB IDP17174
UniProtKB Entry NameAATC_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0010371|Aspartate aminotransferase, cytoplasmic (GOT1)
ATGGCACCTCCGTCAGTCTTTGCCGAGGTTCCGCAGGCCCAGCCTGTCCTGGTCTTCAAG
CTCACTGCCGACTTCAGGGAGGATCCGGACCCCCGCAAGGTCAACCTGGGAGTGGGAGCA
TATCGCACGGATGACTGCCATCCCTGGGTTTTGCCAGTAGTGAAGAAAGTGGAGCAGAAG
ATTGCTAATGACAATAGCCTAAATCACGAGTATCTGCCAATCCTGGGCCTGGCTGAGTTC
CGGAGCTGTGCTTCTCGTCTTGCCCTTGGGGATGACAGCCCAGCACTCAAGGAGAAGCGG
GTAGGAGGTGTGCAATCTTTGGGGGGAACAGGTGCACTTCGAATTGGAGCTGATTTCTTA
GCGCGTTGGTACAATGGAACAAACAACAAGAACACACCTGTCTATGTGTCCTCACCAACC
TGGGAGAATCACAATGCTGTGTTTTCCGCTGCTGGTTTTAAAGACATTCGGTCCTATCGC
TACTGGGATGCAGAGAAGAGAGGATTGGACCTCCAGGGCTTCCTGAATGATCTGGAGAAT
GCTCCTGAGTTCTCCATTGTTGTCCTCCACGCCTGTGCACACAACCCAACTGGGATTGAC
CCAACTCCGGAGCAGTGGAAGCAGATTGCTTCTGTCATGAAGCACCGGTTTCTGTTCCCC
TTCTTTGACTCAGCCTATCAGGGCTTCGCATCTGGAAACCTGGAGAGAGATGCCTGGGCC
ATTCGCTATTTTGTGTCTGAAGGCTTCGAGTTCTTCTGTGCCCAGTCCTTCTCCAAGAAC
TTCGGGCTCTACAATGAGAGAGTCGGGAATCTGACTGTGGTTGGAAAAGAACCTGAGAGC
ATCCTGCAAGTCCTTTCCCAGATGGAGAAGATCGTGCGGATTACTTGGTCCAATCCCCCC
GCCCAGGGAGCACGAATTGTGGCCAGCACCCTCTCTAACCCTGAGCTCTTTGAGGAATGG
ACAGGTAATGTGAAGACAATGGCTGACCGGATTCTGACCATGAGATCTGAACTCAGGGCA
CGACTAGAAGCCCTCAAAACCCCTGGGACCTGGAACCACATCACTGATCAAATTGGCATG
TTCAGCTTCACTGGGTTGAACCCCAAGCAGGTTGAGTATCTGGTCAATGAAAAGCACATC
TACCTGCTGCCAAGTGGTCGAATCAACGTGAGTGGCTTAACCACCAAAAATCTAGATTAC
GTGGCCACCTCCATCCATGAAGCAGTCACCAAAATCCAGTGA
GenBank Gene IDM37400
GeneCard IDNone
GenAtlas IDGOT1
HGNC IDHGNC:4432
Chromosome Location10
Locus10q24.1-q25.1
References
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Related FRC

FRCD ID Name Exact Mass Structure




F05242



L-Cysteine


121.154