ATP-dependent 6-phosphofructokinase, liver type

NameATP-dependent 6-phosphofructokinase, liver type
Synonyms2.7.1.11 6-phosphofructokinase type B ATP-PFK PFK-B Phosphofructo-1-kinase isozyme B Phosphohexokinase
Gene NamePFKL
OrganismHuman
Amino acid sequence
>lcl|BSEQ0008943|ATP-dependent 6-phosphofructokinase, liver type
MAAVDLEKLRASGAGKAIGVLTSGGDAQGMNAAVRAVTRMGIYVGAKVFLIYEGYEGLVE
GGENIKQANWLSVSNIIQLGGTIIGSARCKAFTTREGRRAAAYNLVQHGITNLCVIGGDG
SLTGANIFRSEWGSLLEELVAEGKISETTARTYSHLNIAGLVGSIDNDFCGTDMTIGTDS
ALHRIMEVIDAITTTAQSHQRTFVLEVMGRHCGYLALVSALASGADWLFIPEAPPEDGWE
NFMCERLGETRSRGSRLNIIIIAEGAIDRNGKPISSSYVKDLVVQRLGFDTRVTVLGHVQ
RGGTPSAFDRILSSKMGMEAVMALLEATPDTPACVVTLSGNQSVRLPLMECVQMTKEVQK
AMDDKRFDEATQLRGGSFENNWNIYKLLAHQKPPKEKSNFSLAILNVGAPAAGMNAAVRS
AVRTGISHGHTVYVVHDGFEGLAKGQVQEVGWHDVAGWLGRGGSMLGTKRTLPKGQLESI
VENIRIYGIHALLVVGGFEAYEGVLQLVEARGRYEELCIVMCVIPATISNNVPGTDFSLG
SDTAVNAAMESCDRIKQSASGTKRRVFIVETMGGYCGYLATVTGIAVGADAAYVFEDPFN
IHDLKVNVEHMTEKMKTDIQRGLVLRNEKCHDYYTTEFLYNLYSSEGKGVFDCRTNVLGH
LQQGGAPTPFDRNYGTKLGVKAMLWLSEKLREVYRKGRVFANAPDSACVIGLKKKAVAFS
PVTELKKDTDFEHRMPREQWWLSLRLMLKMLAQYRISMAAYVSGELEHVTRRTLSMDKGF
Number of residues780
Molecular Weight85017.825
Theoretical pINone
GO Classification
Functions
    identical protein binding
ATP binding
metal ion binding
6-phosphofructokinase activity
kinase binding
fructose-6-phosphate binding
fructose binding
Processes
    fructose 1,6-bisphosphate metabolic process
protein homotetramerization
glycolytic process
negative regulation of insulin secretion
canonical glycolysis
response to glucose
small molecule metabolic process
protein oligomerization
carbohydrate metabolic process
fructose 6-phosphate metabolic process
glucose metabolic process
Components
    membrane
6-phosphofructokinase complex
cytosol
extracellular exosome
General FunctionMetal ion binding
Specific FunctionCatalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP17858
UniProtKB Entry NamePFKAL_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0021726|ATP-dependent 6-phosphofructokinase, liver type (PFKL)
ATGAGAAGCATGTGTAACCAGGGTAGAGGTCGAGAGTCCTCTCGTGGGGGTCTCCATGTT
CAAGGGAGCTGCCGAGGCTTGAGCAGGAGCCCCCAGCAGGAAACTGGCTTTGCCAAGGCC
CCCGCTGGGACAGACTGTTTCTTTCACTGCAGTCCTGGGAGCCGAGGGCAAGGGGACAGG
AAAGAGGAAGTGACCTCAGAGCCTGGTGGCACCAGCATCATGTCCAGGCTGGGGGGCATG
AACGCTGCTGTCCGGGCTGTGACGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTC
ATCTACGAGGGCTATGAGGGCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGG
CTGAGCGTCTCCAACATCATCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAG
GCCTTTACCACCAGGGAGGGGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATC
ACCAACCTGTGCGTCATCGGCGGGGATGGCAGCCTCACAGGTGCCAACATCTTCCGCAGC
GAGTGGGGCAGCCTGCTGGAGGAGCTGGTGGCGGAAGGTAAGATCTCAGAGACTACAGCC
CGGACCTACTCGCACCTGAACATCGCGGGCCTAGTGGGCTCCATCGATAACGACTTCTGC
GGCACCGACATGACCATCGGCACGGACTCGGCCCTCCACCGCATCATGGAGGTCATCGAT
GCCATCACCACCACTGCCCAGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCGG
CACTGCGGGTACCTGGCGCTGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATC
CCCGAGGCTCCACCCGAGGACGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGTGAGACT
CGGAGCCGTGGGTCCCGACTGAACATCATCATCATCGCTGAGGGTGCCATTGACCGCAAC
GGGAAGCCCATCTCGTCCAGCTACGTGAAGGACCTGGTGGTTCAGAGGCTGGGCTTCGAC
ACCCGTGTAACTGTGCTGGGCCACGTGCAGCGGGGAGGGACGCCCTCTGCCTTCGACCGG
ATCCTGAGCAGCAAGATGGGCATGGAGGCGGTGATGGCGCTGCTGGAAGCCACGCCTGAC
ACGCCGGCCTGCGTGGTCACCCTCTCGGGGAACCAGTCAGTGCGGCTGCCCCTCATGGAG
TGCGTGCAGATGACCAAGGAAGTGCAGAAAGCCATGGATGACAAGAGGTTTGACGAGGCC
ACCCAGCTCCGTGGTGGGAGCTTCGAGAACAACTGGAACATTTACAAGCTCCTCGCCCAC
CAGAAGCCCCCCAAGGAGAAGTCTAACTTCTCCCTGGCCATCCTGAATGTGGGGGCCCCG
GCGGCTGGCATGAATGCGGCCGTGCGCTCGGCGGTGCGGACCGGCATCTCCCATGGACAC
ACAGTATACGTGGTGCACGATGGCTTCGAAGGCCTAGCCAAGGGTCAGGTGCAAGAAGTA
GGCTGGCACGACGTGGCCGGCTGGTTGGGGCGTGGTGGCTCCATGCTGGGGACCAAGAGG
ACCCTGCCCAAGGGCCAGCTGGAGTCCATTGTGGAGAACATCCGCATCTATGGTATTCAC
GCCCTGCTGGTGGTCGGTGGGTTTGAGGCCTATGAAGGGGTGCTGCAGCTGGTGGAGGCT
CGCGGGCGCTACGAGGAGCTCTGCATCGTCATGTGTGTCATCCCAGCCACCATCAGCAAC
AACGTCCCTGGCACCGACTTCAGCCTGGGCTCCGACACTGCTGTAAATGCCGCCATGGAG
AGCTGTGACCGCATCAAACAGTCTGCCTCGGGGACCAAGCGCCGTGTGTTCATCGTGGAG
ACCATGGGGGGTTACTGTGGCTACCTGGCCACCGTGACTGGCATTGCTGTGGGGGCCGAC
GCCGCCTACGTCTTCGAGGACCCTTTCAACATCCACGACTTAAAGGTCAACGTGGAGCAC
ATGACGGAGAAGATGAAGACAGACATTCAGAGGGGCCTGGTGCTGCGGAACGAGAAGTGC
CATGACTACTACACCACGGAGTTCCTGTACAACCTGTACTCATCAGAGGGCAAGGGCGTC
TTCGACTGCAGGACCAATGTCCTGGGCCACCTGCAGCAGGGTGGCGCTCCAACCCCCTTT
GACCGGAACTATGGGACCAAGCTGGGGGTGAAGGCCATGCTGTGGTTGTCGGAGAAGCTG
CGCGAGGTTTACCGCAAGGGACGGGTGTTCGCCAATGCCCCAGACTCGGCCTGCGTGATC
GGCCTGAAGAAGAAGGCGGTGGCCTTCAGCCCCGTCACTGAGCTCAAGAAAGACACTGAT
TTCGAGCACCGCATGCCACGGGAGCAGTGGTGGCTGAGCCTGCGGCTCATGCTGAAGATG
CTGGCACAATACCGCATCAGTATGGCCGCCTACGTGTCAGGGGAGCTGGAGCACGTGACC
CGCCGCACCCTGAGCATGGACAAGGGCTTCTGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC IDHGNC:8876
Chromosome Location21
LocusNone
References
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  2. Elson A, Levanon D, Brandeis M, Dafni N, Bernstein Y, Danciger E, Groner Y: The structure of the human liver-type phosphofructokinase gene. Genomics. 1990 May;7(1):47-56.[2139864 ]
  3. Gevaert K, Goethals M, Martens L, Van Damme J, Staes A, Thomas GR, Vandekerckhove J: Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides. Nat Biotechnol. 2003 May;21(5):566-9. Epub 2003 Mar 31.[12665801 ]
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  5. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17.[21269460 ]
  6. Bienvenut WV, Sumpton D, Martinez A, Lilla S, Espagne C, Meinnel T, Giglione C: Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-alpha-acetylation features. Mol Cell Proteomics. 2012 Jun;11(6):M111.015131. doi: 10.1074/mcp.M111.015131. Epub 2012 Jan 5.[22223895 ]
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  8. Yi W, Clark PM, Mason DE, Keenan MC, Hill C, Goddard WA 3rd, Peters EC, Driggers EM, Hsieh-Wilson LC: Phosphofructokinase 1 glycosylation regulates cell growth and metabolism. Science. 2012 Aug 24;337(6097):975-80. doi: 10.1126/science.1222278.[22923583 ]
  9. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22.[24275569 ]
  10. Hattori M, Fujiyama A, Taylor TD, Watanabe H, Yada T, Park HS, Toyoda A, Ishii K, Totoki Y, Choi DK, Groner Y, Soeda E, Ohki M, Takagi T, Sakaki Y, Taudien S, Blechschmidt K, Polley A, Menzel U, Delabar J, Kumpf K, Lehmann R, Patterson D, Reichwald K, Rump A, Schillhabel M, Schudy A, Zimmermann W, Rosenthal A, Kudoh J, Schibuya K, Kawasaki K, Asakawa S, Shintani A, Sasaki T, Nagamine K, Mitsuyama S, Antonarakis SE, Minoshima S, Shimizu N, Nordsiek G, Hornischer K, Brant P, Scharfe M, Schon O, Desario A, Reichelt J, Kauer G, Blocker H, Ramser J, Beck A, Klages S, Hennig S, Riesselmann L, Dagand E, Haaf T, Wehrmeyer S, Borzym K, Gardiner K, Nizetic D, Francis F, Lehrach H, Reinhardt R, Yaspo ML: The DNA sequence of human chromosome 21. Nature. 2000 May 18;405(6784):311-9.[10830953 ]
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