Kinesin-1 heavy chain

NameKinesin-1 heavy chain
SynonymsConventional kinesin heavy chain KNS KNS1 Ubiquitous kinesin heavy chain UKHC
Gene NameKIF5B
OrganismHuman
Amino acid sequence
>lcl|BSEQ0012167|Kinesin-1 heavy chain
MADLAECNIKVMCRFRPLNESEVNRGDKYIAKFQGEDTVVIASKPYAFDRVFQSSTSQEQ
VYNDCAKKIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPEGMGIIPRIVQDIFNYIY
SMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLSVHEDKNRVPYVKGCTERFVCSPDEVM
DTIDEGKSNRHVAVTNMNEHSSRSHSIFLINVKQENTQTEQKLSGKLYLVDLAGSEKVSK
TGAEGAVLDEAKNINKSLSALGNVISALAEGSTYVPYRDSKMTRILQDSLGGNCRTTIVI
CCSPSSYNESETKSTLLFGQRAKTIKNTVCVNVELTAEQWKKKYEKEKEKNKILRNTIQW
LENELNRWRNGETVPIDEQFDKEKANLEAFTVDKDITLTNDKPATAIGVIGNFTDAERRK
CEEEIAKLYKQLDDKDEEINQQSQLVEKLKTQMLDQEELLASTRRDQDNMQAELNRLQAE
NDASKEEVKEVLQALEELAVNYDQKSQEVEDKTKEYELLSDELNQKSATLASIDAELQKL
KEMTNHQKKRAAEMMASLLKDLAEIGIAVGNNDVKQPEGTGMIDEEFTVARLYISKMKSE
VKTMVKRCKQLESTQTESNKKMEENEKELAACQLRISQHEAKIKSLTEYLQNVEQKKRQL
EESVDALSEELVQLRAQEKVHEMEKEHLNKVQTANEVKQAVEQQIQSHRETHQKQISSLR
DEVEAKAKLITDLQDQNQKMMLEQERLRVEHEKLKATDQEKSRKLHELTVMQDRREQARQ
DLKGLEETVAKELQTLHNLRKLFVQDLATRVKKSAEIDSDDTGGSAAQKQKISFLENNLE
QLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALESALKEAKENASRDRKRYQQEVD
RIKEAVRSKNMARRGHSAQIAKPIRPGQHPAASPTHPSAIRGGGAFVQNSQPVAVRGGGG
KQV
Number of residues963
Molecular Weight109684.15
Theoretical pI6.46
GO Classification
Functions
    ATP binding
microtubule motor activity
microtubule binding
ATP-dependent microtubule motor activity, plus-end-directed
Processes
    cytoskeleton-dependent intracellular transport
plus-end-directed vesicle transport along microtubule
regulation of membrane potential
positive regulation of establishment of protein localization to plasma membrane
microtubule-based movement
positive regulation of potassium ion transport
stress granule disassembly
protein localization
vesicle transport along microtubule
positive regulation of synaptic transmission, GABAergic
axon guidance
cytoplasm organization
cellular protein metabolic process
Components
    microtubule
vesicle
microtubule organizing center
perinuclear region of cytoplasm
ciliary rootlet
neuron projection
kinesin complex
cytoplasm
endocytic vesicle
membrane
General FunctionMicrotubule motor activity
Specific FunctionMicrotubule-dependent motor required for normal distribution of mitochondria and lysosomes.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP33176
UniProtKB Entry NameKINH_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0012168|Kinesin-1 heavy chain (KIF5B)
ATGGCGGACCTGGCCGAGTGCAACATCAAAGTGATGTGTCGCTTCAGACCTCTCAACGAG
TCTGAAGTGAACCGCGGCGACAAGTACATCGCCAAGTTTCAGGGAGAAGACACGGTCGTG
ATCGCGTCCAAGCCTTATGCATTTGATCGGGTGTTCCAGTCAAGCACATCTCAAGAGCAA
GTGTATAATGACTGTGCAAAGAAGATTGTTAAAGATGTACTTGAAGGATATAATGGAACA
ATATTTGCATATGGACAAACATCCTCTGGGAAGACACACACAATGGAGGGTAAACTTCAT
GATCCAGAAGGCATGGGAATTATTCCAAGAATAGTGCAAGATATTTTTAATTATATTTAC
TCCATGGATGAAAATTTGGAATTTCATATTAAGGTTTCATATTTTGAAATATATTTGGAT
AAGATAAGGGACCTGTTAGATGTTTCAAAGACCAACCTTTCAGTTCATGAAGACAAAAAC
CGAGTTCCCTATGTAAAGGGGTGCACAGAGCGTTTTGTATGTAGTCCAGATGAAGTTATG
GATACCATAGATGAAGGAAAATCCAACAGACATGTAGCAGTTACAAATATGAATGAACAT
AGCTCTAGGAGTCACAGTATATTTCTTATTAATGTCAAACAAGAGAACACACAAACGGAA
CAAAAGCTGAGTGGAAAACTTTATCTGGTTGATTTAGCTGGTAGTGAAAAGGTTAGTAAA
ACTGGAGCTGAAGGTGCTGTGCTGGATGAAGCTAAAAACATCAACAAGTCACTTTCTGCT
CTTGGAAATGTTATTTCTGCTTTGGCTGAGGGTAGTACATATGTTCCATATCGAGATAGT
AAAATGACAAGAATCCTTCAAGATTCATTAGGTGGCAACTGTAGAACCACTATTGTAATT
TGCTGCTCTCCATCATCATACAATGAGTCTGAAACAAAATCTACACTCTTATTTGGCCAA
AGGGCCAAAACAATTAAGAACACAGTTTGTGTCAATGTGGAGTTAACTGCAGAACAGTGG
AAAAAGAAGTATGAAAAAGAAAAAGAAAAAAATAAGATCCTGCGGAACACTATTCAGTGG
CTTGAAAATGAGCTCAACAGATGGCGTAATGGGGAGACGGTGCCTATTGATGAACAGTTT
GACAAAGAGAAAGCCAACTTGGAAGCTTTCACAGTGGATAAAGATATTACTCTTACCAAT
GATAAACCAGCAACCGCAATTGGAGTTATAGGAAATTTTACTGATGCTGAAAGAAGAAAG
TGTGAAGAAGAAATTGCTAAATTATACAAACAGCTTGATGACAAGGATGAAGAAATTAAC
CAGCAAAGTCAACTGGTAGAGAAACTGAAGACGCAAATGTTGGATCAGGAGGAGCTTTTG
GCATCTACCAGAAGGGATCAAGACAATATGCAAGCTGAGCTGAATCGCCTTCAAGCAGAA
AATGATGCCTCTAAAGAAGAAGTGAAAGAAGTTTTACAGGCCCTAGAAGAACTTGCTGTC
AATTATGATCAGAAGTCTCAGGAAGTTGAAGACAAAACTAAGGAATATGAATTGCTTAGT
GATGAATTGAATCAGAAATCGGCAACTTTAGCGAGTATAGATGCTGAGCTTCAGAAACTT
AAGGAAATGACCAACCACCAGAAAAAACGAGCAGCTGAGATGATGGCATCTTTACTAAAA
GACCTTGCAGAAATAGGAATTGCTGTGGGAAATAATGATGTAAAGCAGCCTGAGGGAACT
GGCATGATAGATGAAGAGTTCACTGTTGCAAGACTCTACATTAGCAAAATGAAGTCAGAA
GTAAAAACCATGGTGAAACGTTGCAAGCAGTTAGAAAGCACACAAACTGAGAGCAACAAA
AAAATGGAAGAAAATGAAAAGGAGTTAGCAGCATGTCAGCTTCGTATCTCTCAACATGAA
GCCAAAATCAAGTCATTGACTGAATACCTTCAAAATGTGGAACAAAAGAAAAGACAGTTG
GAGGAATCTGTCGATGCCCTCAGTGAAGAACTAGTCCAGCTTCGAGCACAAGAGAAAGTC
CATGAAATGGAAAAGGAGCACTTAAATAAGGTTCAGACTGCAAATGAAGTTAAGCAAGCT
GTTGAACAGCAGATCCAGAGCCATAGAGAAACTCATCAAAAACAGATCAGTAGTTTGAGA
GATGAAGTAGAAGCAAAAGCAAAACTTATTACTGATCTTCAAGACCAAAACCAGAAAATG
ATGTTAGAGCAGGAACGTCTAAGAGTAGAACATGAGAAGTTGAAAGCCACAGATCAGGAA
AAGAGCAGAAAACTACATGAACTTACGGTTATGCAAGATAGACGAGAACAAGCAAGACAA
GACTTGAAGGGTTTGGAAGAGACAGTGGCAAAAGAACTTCAGACTTTACACAACCTGCGC
AAACTCTTTGTTCAGGACCTGGCTACAAGAGTTAAAAAGAGTGCTGAGATTGATTCTGAT
GACACCGGAGGCAGCGCTGCTCAGAAGCAAAAAATCTCCTTTCTTGAAAATAATCTTGAA
CAGCTCACTAAAGTGCACAAACAGTTGGTACGTGATAATGCAGATCTCCGCTGTGAACTT
CCTAAGTTGGAAAAGCGACTTCGAGCTACAGCTGAGAGAGTGAAAGCTTTGGAATCAGCA
CTGAAAGAAGCTAAAGAAAATGCATCTCGTGATCGCAAACGCTATCAGCAAGAAGTAGAT
CGCATAAAGGAAGCAGTCAGGTCAAAGAATATGGCCAGAAGAGGGCATTCTGCACAGATT
GCTAAACCTATTCGTCCCGGGCAACATCCAGCAGCTTCTCCAACTCACCCAAGTGCAATT
CGTGGAGGAGGTGCATTTGTTCAGAACAGCCAGCCAGTGGCAGTGCGAGGTGGAGGAGGC
AAACAAGTGTAA
GenBank Gene IDX65873
GeneCard IDNone
GenAtlas IDKIF5B
HGNC IDHGNC:6324
Chromosome Location10
Locus10pter-q22.1
References
  1. Navone F, Niclas J, Hom-Booher N, Sparks L, Bernstein HD, McCaffrey G, Vale RD: Cloning and expression of a human kinesin heavy chain gene: interaction of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells. J Cell Biol. 1992 Jun;117(6):1263-75.[1607388 ]
  2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, Ko MS, Kawakami K, Suzuki Y, Sugano S, Gruber CE, Smith MR, Simmons B, Moore T, Waterman R, Johnson SL, Ruan Y, Wei CL, Mathavan S, Gunaratne PH, Wu J, Garcia AM, Hulyk SW, Fuh E, Yuan Y, Sneed A, Kowis C, Hodgson A, Muzny DM, McPherson J, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madari A, Young AC, Wetherby KD, Granite SJ, Kwong PN, Brinkley CP, Pearson RL, Bouffard GG, Blakesly RW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Griffith M, Griffith OL, Krzywinski MI, Liao N, Morin R, Palmquist D, Petrescu AS, Skalska U, Smailus DE, Stott JM, Schnerch A, Schein JE, Jones SJ, Holt RA, Baross A, Marra MA, Clifton S, Makowski KA, Bosak S, Malek J: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 2004 Oct;14(10B):2121-7.[15489334 ]
  3. Su Q, Cai Q, Gerwin C, Smith CL, Sheng ZH: Syntabulin is a microtubule-associated protein implicated in syntaxin transport in neurons. Nat Cell Biol. 2004 Oct;6(10):941-53. Epub 2004 Sep 19.[15459722 ]
  4. Boucrot E, Henry T, Borg JP, Gorvel JP, Meresse S: The intracellular fate of Salmonella depends on the recruitment of kinesin. Science. 2005 May 20;308(5725):1174-8.[15905402 ]
  5. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell. 2006 Nov 3;127(3):635-48.[17081983 ]
  6. Vidal RL, Ramirez OA, Sandoval L, Koenig-Robert R, Hartel S, Couve A: Marlin-1 and conventional kinesin link GABAB receptors to the cytoskeleton and regulate receptor transport. Mol Cell Neurosci. 2007 Jul;35(3):501-12. Epub 2007 May 1.[17532644 ]
  7. Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475.[20068231 ]
  8. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17.[21269460 ]
  9. Bienvenut WV, Sumpton D, Martinez A, Lilla S, Espagne C, Meinnel T, Giglione C: Comparative large scale characterization of plant versus mammal proteins reveals similar and idiosyncratic N-alpha-acetylation features. Mol Cell Proteomics. 2012 Jun;11(6):M111.015131. doi: 10.1074/mcp.M111.015131. Epub 2012 Jan 5.[22223895 ]
  10. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54. doi: 10.1073/pnas.1210303109. Epub 2012 Jul 18.[22814378 ]
  11. Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics. 2014 Jan 16;96:253-62. doi: 10.1016/j.jprot.2013.11.014. Epub 2013 Nov 22.[24275569 ]
  12. Kull FJ, Sablin EP, Lau R, Fletterick RJ, Vale RD: Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature. 1996 Apr 11;380(6574):550-5.[8606779 ]
  13. Sindelar CV, Budny MJ, Rice S, Naber N, Fletterick R, Cooke R: Two conformations in the human kinesin power stroke defined by X-ray crystallography and EPR spectroscopy. Nat Struct Biol. 2002 Nov;9(11):844-8.[12368902 ]
  14. Sindelar CV, Downing KH: The beginning of kinesin's force-generating cycle visualized at 9-A resolution. J Cell Biol. 2007 May 7;177(3):377-85. Epub 2007 Apr 30.[17470637 ]
  15. Gorbea C, Pratt G, Ustrell V, Bell R, Sahasrabudhe S, Hughes RE, Rechsteiner M: A protein interaction network for Ecm29 links the 26 S proteasome to molecular motors and endosomal components. J Biol Chem. 2010 Oct 8;285(41):31616-33. doi: 10.1074/jbc.M110.154120. Epub 2010 Aug 3.[20682791 ]
  16. Deloukas P, Earthrowl ME, Grafham DV, Rubenfield M, French L, Steward CA, Sims SK, Jones MC, Searle S, Scott C, Howe K, Hunt SE, Andrews TD, Gilbert JG, Swarbreck D, Ashurst JL, Taylor A, Battles J, Bird CP, Ainscough R, Almeida JP, Ashwell RI, Ambrose KD, Babbage AK, Bagguley CL, Bailey J, Banerjee R, Bates K, Beasley H, Bray-Allen S, Brown AJ, Brown JY, Burford DC, Burrill W, Burton J, Cahill P, Camire D, Carter NP, Chapman JC, Clark SY, Clarke G, Clee CM, Clegg S, Corby N, Coulson A, Dhami P, Dutta I, Dunn M, Faulkner L, Frankish A, Frankland JA, Garner P, Garnett J, Gribble S, Griffiths C, Grocock R, Gustafson E, Hammond S, Harley JL, Hart E, Heath PD, Ho TP, Hopkins B, Horne J, Howden PJ, Huckle E, Hynds C, Johnson C, Johnson D, Kana A, Kay M, Kimberley AM, Kershaw JK, Kokkinaki M, Laird GK, Lawlor S, Lee HM, Leongamornlert DA, Laird G, Lloyd C, Lloyd DM, Loveland J, Lovell J, McLaren S, McLay KE, McMurray A, Mashreghi-Mohammadi M, Matthews L, Milne S, Nickerson T, Nguyen M, Overton-Larty E, Palmer SA, Pearce AV, Peck AI, Pelan S, Phillimore B, Porter K, Rice CM, Rogosin A, Ross MT, Sarafidou T, Sehra HK, Shownkeen R, Skuce CD, Smith M, Standring L, Sycamore N, Tester J, Thorpe A, Torcasso W, Tracey A, Tromans A, Tsolas J, Wall M, Walsh J, Wang H, Weinstock K, West AP, Willey DL, Whitehead SL, Wilming L, Wray PW, Young L, Chen Y, Lovering RC, Moschonas NK, Siebert R, Fechtel K, Bentley D, Durbin R, Hubbard T, Doucette-Stamm L, Beck S, Smith DR, Rogers J: The DNA sequence and comparative analysis of human chromosome 10. Nature. 2004 May 27;429(6990):375-81.[15164054 ]
  17. Gauci S, Helbig AO, Slijper M, Krijgsveld J, Heck AJ, Mohammed S: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem. 2009 Jun 1;81(11):4493-501. doi: 10.1021/ac9004309.[19413330 ]

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