MAP kinase-activated protein kinase 2

NameMAP kinase-activated protein kinase 2
Synonyms2.7.11.1 MAPK-activated protein kinase 2
Gene NameMAPKAPK2
OrganismHuman
Amino acid sequence
>lcl|BSEQ0002725|MAP kinase-activated protein kinase 2
MLSNSQGQSPPVPFPAPAPPPQPPTPALPHPPAQPPPPPPQQFPQFHVKSGLQIKKNAII
DDYKVTSQVLGLGINGKVLQIFNKRTQEKFALKMLQDCPKARREVELHWRASQCPHIVRI
VDVYENLYAGRKCLLIVMECLDGGELFSRIQDRGDQAFTEREASEIMKSIGEAIQYLHSI
NIAHRDVKPENLLYTSKRPNAILKLTDFGFAKETTSHNSLTTPCYTPYYVAPEVLGPEKY
DKSCDMWSLGVIMYILLCGYPPFYSNHGLAISPGMKTRIRMGQYEFPNPEWSEVSEEVKM
LIRNLLKTEPTQRMTITEFMNHPWIMQSTKVPQTPLHTSRVLKEDKERWEDVKEEMTSAL
ATMRVDYEQIKIKKIEDASNPLLLKRRKKARALEAAALAH
Number of residues400
Molecular Weight45567.415
Theoretical pI8.92
GO Classification
Functions
    calcium-dependent protein serine/threonine kinase activity
calmodulin binding
calmodulin-dependent protein kinase activity
signal transducer activity
ATP binding
mitogen-activated protein kinase binding
protein kinase activity
protein serine/threonine kinase activity
Processes
    response to stress
toll-like receptor 2 signaling pathway
protein phosphorylation
cellular response to vascular endothelial growth factor stimulus
gene expression
toll-like receptor 3 signaling pathway
Ras protein signal transduction
macropinocytosis
regulation of mRNA stability
small molecule metabolic process
toll-like receptor 4 signaling pathway
vascular endothelial growth factor receptor signaling pathway
p38MAPK cascade
toll-like receptor 5 signaling pathway
response to lipopolysaccharide
regulation of tumor necrosis factor production
toll-like receptor 9 signaling pathway
inner ear development
3'-UTR-mediated mRNA stabilization
cellular response to heat
toll-like receptor signaling pathway
protein autophosphorylation
regulation of interleukin-6 production
MyD88-dependent toll-like receptor signaling pathway
toll-like receptor TLR1
activation of MAPK activity
MyD88-independent toll-like receptor signaling pathway
cellular response to DNA damage stimulus
toll-like receptor TLR6
arachidonic acid metabolic process
regulation of cellular response to heat
innate immune response
TRIF-dependent toll-like receptor signaling pathway
leukotriene metabolic process
stress-activated MAPK cascade
MAPK cascade
positive regulation of tumor necrosis factor biosynthetic process
response to cytokine
inflammatory response
toll-like receptor 10 signaling pathway
neurotrophin TRK receptor signaling pathway
G2 DNA damage checkpoint
peptidyl-serine phosphorylation
Components
    centrosome
cytosol
cytoplasm
extracellular exosome
nucleoplasm
nucleus
General FunctionSignal transducer activity
Specific FunctionStress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.
Transmembrane Regions
GenBank Protein ID530090
UniProtKB IDP49137
UniProtKB Entry NameMAPK2_HUMAN
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0021291|MAP kinase-activated protein kinase 2 (MAPKAPK2)
ATGCTGTCCAACTCCCAGGGCCAGAGCCCGCCGGTGCCGTTCCCCGCCCCGGCCCCGCCG
CCGCAGCCCCCCACCCCTGCCCTGCCGCACCCCCCGGCGCAGCCGCCGCCGCCGCCCCCG
CAGCAGTTCCCGCAGTTCCACGTCAAGTCCGGCCTGCAGATCAAGAAGAACGCCATCATC
GATGACTACAAGGTCACCAGCCAGGTCCTGGGGCTGGGCATCAACGGCAAAGTTTTGCAG
ATCTTCAACAAGAGGACCCAGGAGAAATTCGCCCTCAAAATGCTTCAGGACTGCCCCAAG
GCCCGCAGGGAGGTGGAGCTGCACTGGCGGGCCTCCCAGTGCCCGCACATCGTACGGATC
GTGGATGTGTACGAGAATCTGTACGCAGGGAGGAAGTGCCTGCTGATTGTCATGGAATGT
TTGGACGGTGGAGAACTCTTTAGCCGAATCCAGGATCGAGGAGACCAGGCATTCACAGAA
AGAGAAGCATCCGAAATCATGAAGAGCATCGGTGAGGCCATCCAGTATCTGCATTCAATC
AACATTGCCCATCGGGATGTCAAGCCTGAGAATCTCTTATACACCTCCAAAAGGCCCAAC
GCCATCCTGAAACTCACTGACTTTGGCTTTGCCAAGGAAACCACCAGCCACAACTCTTTG
ACCACTCCTTGTTATACACCGTACTATGTGGCTCCAGAAGTGCTGGGTCCAGAGAAGTAT
GACAAGTCCTGTGACATGTGGTCCCTGGGTGTCATCATGTACATCCTGCTGTGTGGGTAT
CCCCCCTTCTACTCCAACCACGGCCTTGCCATCTCTCCGGGCATGAAGACTCGCATCCGA
ATGGGCCAGTATGAATTTCCCAACCCAGAATGGTCAGAAGTATCAGAGGAAGTGAAGATG
CTCATTCGGAATCTGCTGAAAACAGAGCCCACCCAGAGAATGACCATCACCGAGTTTATG
AACCACCCTTGGATCATGCAATCAACAAAGGTCCCTCAAACCCCACTGCACACCAGCCGG
GTCCTGAAGGAGGACAAGGAGCGGTGGGAGGATGTCAAGGGGTGTCTTCATGACAAGAAC
AGCGACCAGGCCACTTGGCTGACCAGGTTGTGA
GenBank Gene IDU12779
GeneCard IDNone
GenAtlas IDMAPKAPK2
HGNC IDHGNC:6887
Chromosome Location1
Locus1q32
References
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