DNA-directed RNA polymerase II subunit RPB7

NameDNA-directed RNA polymerase II subunit RPB7
SynonymsDNA-directed RNA polymerase II subunit G RNA polymerase II 19 kDa subunit RNA polymerase II subunit B7 RPB19 RPB7
Gene NamePOLR2G
OrganismHuman
Amino acid sequence
>lcl|BSEQ0019705|DNA-directed RNA polymerase II subunit RPB7
MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ
PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD
PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS
Number of residues172
Molecular Weight19294.195
Theoretical pINone
GO Classification
Functions
    DNA-directed RNA polymerase activity
single-stranded RNA binding
single-stranded DNA binding
translation initiation factor binding
Processes
    transcription initiation from RNA polymerase II promoter
mRNA splicing, via spliceosome
piRNA metabolic process
apoptotic process
positive regulation of translational initiation
somatic stem cell population maintenance
positive regulation of viral transcription
DNA repair
transcription-coupled nucleotide-excision repair
RNA splicing
viral process
transcription elongation from RNA polymerase II promoter
nucleotide-excision repair
nuclear-transcribed mRNA catabolic process, exonucleolytic
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening
transcription from RNA polymerase II promoter
7-methylguanosine mRNA capping
gene silencing by RNA
gene expression
Components
    nucleoplasm
nucleus
cytoplasmic mRNA processing body
DNA-directed RNA polymerase II, core complex
General FunctionTranslation initiation factor binding
Specific FunctionDNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP62487
UniProtKB Entry NameRPB7_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0019706|DNA-directed RNA polymerase II subunit RPB7 (POLR2G)
ATGTTCTACCATATCTCCCTAGAGCACGAAATCCTGCTGCACCCGCGCTACTTCGGCCCC
AACTTGCTCAACACGGTGAAGCAGAAGCTCTTCACCGAGGTGGAGGGGACCTGCACAGGG
AAGTATGGCTTTGTAATTGCTGTCACCACCATTGACAATATTGGTGCTGGTGTGATCCAG
CCAGGCCGAGGCTTTGTCCTTTATCCAGTTAAGTACAAGGCCATTGTTTTCCGGCCATTT
AAAGGGGAGGTCGTGGATGCTGTTGTCACTCAGGTCAACAAGGTTGGACTCTTCACAGAA
ATTGGGCCCATGTCTTGCTTCATCTCTCGACATTCCATCCCTTCAGAGATGGAGTTTGAT
CCTAACTCCAACCCACCATGTTACAAGACAATGGATGAGGATATTGTGATTCAGCAGGAC
GATGAGATCCGCTTAAAGATTGTGGGGACCCGTGTGGACAAGAATGACATTTTTGCTATT
GGCTCCCTGATGGACGATTACTTGGGGCTTGTAAGCTGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC IDHGNC:9194
Chromosome Location11
LocusNone
References
  1. Khazak V, Sadhale PP, Woychik NA, Brent R, Golemis EA: Human RNA polymerase II subunit hsRPB7 functions in yeast and influences stress survival and cell morphology. Mol Biol Cell. 1995 Jul;6(7):759-75.[7579693 ]
  2. Schoen TJ, Chandrasekharappa SC, Guru SC, Mazuruk K, Chader GJ, Rodriguez IR: Human gene for the RNA polymerase II seventh subunit (hsRPB7): structure, expression and chromosomal localization. Biochim Biophys Acta. 1997 Jul 17;1353(1):39-49.[9256063 ]
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  4. Kershnar E, Wu SY, Chiang CM: Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes. J Biol Chem. 1998 Dec 18;273(51):34444-53.[9852112 ]
  5. Burkard TR, Planyavsky M, Kaupe I, Breitwieser FP, Burckstummer T, Bennett KL, Superti-Furga G, Colinge J: Initial characterization of the human central proteome. BMC Syst Biol. 2011 Jan 26;5:17. doi: 10.1186/1752-0509-5-17.[21269460 ]
  6. Meka H, Werner F, Cordell SC, Onesti S, Brick P: Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II. Nucleic Acids Res. 2005 Nov 10;33(19):6435-44. Print 2005.[16282592 ]
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