5'-AMP-activated protein kinase catalytic subunit alpha-1
| Name | 5'-AMP-activated protein kinase catalytic subunit alpha-1 |
|---|---|
| Synonyms | 2.7.11.1 ACACA kinase Acetyl-CoA carboxylase kinase AMPK subunit alpha-1 AMPK1 HMGCR kinase Hydroxymethylglutaryl-CoA reductase kinase Tau-protein kinase PRKAA1 |
| Gene Name | PRKAA1 |
| Organism | Human |
| Amino acid sequence | >lcl|BSEQ0037059|5'-AMP-activated protein kinase catalytic subunit alpha-1 MRRLSSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNR QKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNG RLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEF LRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDG IFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID DEALKEVCEKFECSEEEVLSCLYNRNHQDPLAVAYHLIIDNRRIMNEAKDFYLATSPPDS FLDDHHLTRPHPERVPFLVAETPRARHTLDELNPQKSKHQGVRKAKWHLGIRSQSRPNDI MAEVCRAIKQLDYEWKVVNPYYLRVRRKNPVTSTYSKMSLQLYQVDSRTYLLDFRSIDDE ITEAKSGTATPQRSGSVSNYRSCQRSDSDAEAQGKSSEVSLTSSVTSLDSSPVDLTPRPG SHTIEFFEMCANLIKILAQ |
| Number of residues | 559 |
| Molecular Weight | 64008.64 |
| Theoretical pI | 7.64 |
| GO Classification |
Functions
histone serine kinase activity chromatin binding tau-protein kinase activity metal ion binding AMP-activated protein kinase activity [acetyl-CoA carboxylase] kinase activity ATP binding [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity protein kinase activity cAMP-dependent protein kinase activity Processes
cellular response to hypoxia response to camptothecin response to caffeine regulation of transcription, DNA-templated cellular response to glucose starvation response to gamma radiation negative regulation of lipid catabolic process cholesterol biosynthetic process activation of MAPK activity Wnt signaling pathway cell cycle arrest positive regulation of glycolytic process insulin receptor signaling pathway cellular response to ethanol regulation of circadian rhythm negative regulation of apoptotic process cellular response to nutrient levels negative regulation of glucosylceramide biosynthetic process rhythmic process cellular response to prostaglandin E stimulus protein phosphorylation cold acclimation fatty acid biosynthetic process negative regulation of TOR signaling fatty acid oxidation response to UV response to hypoxia histone-serine phosphorylation macroautophagy positive regulation of autophagy fatty acid homeostasis gene expression positive regulation of cell proliferation negative regulation of glucose import in response to insulin stimulus positive regulation of gene expression positive regulation of cholesterol biosynthetic process positive regulation of skeletal muscle tissue development transcription initiation from RNA polymerase II promoter intracellular signal transduction signal transduction protein heterooligomerization regulation of energy homeostasis response to activity regulation of peptidyl-serine phosphorylation cellular response to hydrogen peroxide glucose homeostasis regulation of vesicle-mediated transport lipid biosynthetic process glucose metabolic process Components
nucleus nucleotide-activated protein kinase complex intracellular cytoplasm cytosol apical plasma membrane |
| General Function | Tau-protein kinase activity |
| Specific Function | Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1/ULK1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ATG1/ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1. |
| Transmembrane Regions | |
| GenBank Protein ID | 4115829 |
| UniProtKB ID | Q13131 |
| UniProtKB Entry Name | AAPK1_HUMAN |
| Cellular Location | Cytoplasm |
| Gene sequence | >lcl|BSEQ0010471|5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1) ATGCGCAGACTCAGTTCCTGGAGAAAGATGGCGACAGCCGAGAAGCAGAAACACGACGGG CGGGTGAAGATCGGCCACTACATTCTGGGTGACACGCTGGGGGTCGGCACCTTCGGCAAA GTGAAGGTTGGCAAACATGAATTGACTGGGCATAAAGTAGCTGTGAAGATACTCAATCGA CAGAAGATTCGGAGCCTTGATGTGGTAGGAAAAATCCGCAGAGAAATTCAGAACCTCAAG CTTTTCAGGCATCCTCATATAATTAAACTGTACCAGGTCATCAGTACACCATCTGATATT TTCATGGTGATGGAATATGTCTCAGGAGGAGAGCTATTTGATTATATCTGTAAGAATGGA AGGCTGGATGAAAAAGAAAGTCGGCGTCTGTTCCAACAGATCCTTTCTGGTGTGGATTAT TGTCACAGGCATATGGTGGTCCATAGAGATTTGAAACCTGAAAATGTCCTGCTTGATGCA CACATGAATGCAAAGATAGCTGATTTTGGTCTTTCAAACATGATGTCAGATGGTGAATTT TTAAGAACAAGTTGTGGCTCACCCAACTATGCTGCACCAGAAGTAATTTCAGGAAGATTG TATGCAGGCCCAGAGGTAGATATATGGAGCAGTGGGGTTATTCTCTATGCTTTATTATGT GGAACCCTTCCATTTGATGATGACCATGTGCCAACTCTTTTTAAGAAGATATGTGATGGG ATCTTCTATACCCCTCAATATTTAAATCCTTCTGTGATTAGCCTTTTGAAACATATGCTG CAGGTGGATCCCATGAAGAGGGCCACAATCAAAGATATCAGGGAACATGAATGGTTTAAA CAGGACCTTCCAAAATATCTCTTTCCTGAGGATCCATCATATAGTTCAACCATGATTGAT GATGAAGCCTTAAAAGAAGTATGTGAAAAGTTTGAGTGCTCAGAAGAGGAAGTTCTCAGC TGTCTTTACAACAGAAATCACCAGGATCCTTTGGCAGTTGCCTACCATCTCATAATAGAT AACAGGAGAATAATGAATGAAGCCAAAGATTTCTATTTGGCGACAAGCCCACCTGATTCT TTTCTTGATGATCATCACCTGACTCGGCCCCATCCTGAAAGAGTACCATTCTTGGTTGCT GAAACACCAAGGGCACGCCATACCCTTGATGAATTAAATCCACAGAAATCCAAACACCAA GGTGTAAGGAAAGCAAAATGGCATTTAGGAATTAGAAGTCAAAGTCGACCAAATGATATT ATGGCAGAAGTATGTAGAGCAATCAAACAATTGGATTATGAATGGAAGGTTGTAAACCCA TATTATTTGCGTGTACGAAGGAAGAATCCTGTGACAAGCACTTACTCCAAAATGAGTCTA CAGTTATACCAAGTGGATAGTAGAACTTATCTACTGGATTTCCGTAGTATTGATGATGAA ATTACAGAAGCCAAATCAGGGACTGCTACTCCACAGAGATCGGGATCAGTTAGCAACTAT CGATCTTGCCAAAGGAGTGATTCAGATGCTGAGGCTCAAGGAAAATCCTCAGAAGTTTCT CTTACCTCATCTGTGACCTCACTTGACTCTTCTCCTGTTGACCTAACTCCAAGACCTGGA AGTCACACAATAGAATTTTTTGAGATGTGTGCAAATCTAATTAAAATTCTTGCACAATAA |
| GenBank Gene ID | AB022017 |
| GeneCard ID | None |
| GenAtlas ID | PRKAA1 |
| HGNC ID | HGNC:9376 |
| Chromosome Location | 5 |
| Locus | 5p12 |
| References |
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Related FRC
| FRCD ID | Name | Exact Mass | Structure |
|---|---|---|---|
Maneb |
265.284 |
||
1-Hydroxypyrene |
220.271 |
||
Cyclanilide |
274.097 |