NADPH oxidase 4

NameNADPH oxidase 4
Synonyms1.6.3.- Kidney oxidase-1 Kidney superoxide-producing NADPH oxidase KOX-1 Renal NAD(P)H-oxidase RENOX
Gene NameNOX4
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009326|NADPH oxidase 4
MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRAS
ASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHV
AAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTY
AIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTNLDTHPPGCISLNRTSSQNISLP
EYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANFPQTWLWISGPLCLYCAERLY
RYIRSNKPVTIISVMSHPSDVMEIRMVKENFKARPGQYITLHCPSVSALENHPFTLTMCP
TETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEES
LNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLH
NKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGK
TVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS
Number of residues578
Molecular Weight66930.995
Theoretical pINone
GO Classification
Functions
    oxygen sensor activity
nucleotide binding
heme binding
modified amino acid binding
superoxide-generating NADPH oxidase activity
electron carrier activity
NAD(P)H oxidase activity
flavin adenine dinucleotide binding
Processes
    reactive oxygen species metabolic process
positive regulation of MAP kinase activity
bone resorption
cell aging
positive regulation of stress fiber assembly
positive regulation of DNA biosynthetic process
cardiac muscle cell differentiation
positive regulation of apoptotic process
positive regulation of ERK1 and ERK2 cascade
superoxide metabolic process
cellular response to transforming growth factor beta stimulus
positive regulation of smooth muscle cell migration
cellular response to gamma radiation
oxidation-reduction process
negative regulation of cell proliferation
homocysteine metabolic process
inflammatory response
response to hypoxia
cellular response to cAMP
superoxide anion generation
positive regulation of reactive oxygen species metabolic process
positive regulation of protein kinase B signaling
cellular response to glucose stimulus
gene expression
cell morphogenesis
Components
    nucleolus
focal adhesion
perinuclear region of cytoplasm
mitochondrion
endoplasmic reticulum membrane
NADPH oxidase complex
integral component of membrane
stress fiber
apical plasma membrane
General FunctionSuperoxide-generating nadph oxidase activity
Specific FunctionConstitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.
Transmembrane Regions17-37 63-83 104-124 155-175 189-209 425-445
GenBank Protein ID
UniProtKB IDQ9NPH5
UniProtKB Entry NameNOX4_HUMAN
Cellular LocationEndoplasmic reticulum membrane
Gene sequence
>lcl|BSEQ0013803|NADPH oxidase 4 (NOX4)
ATGGCTGTGTCCTGGAGGAGCTGGCTCGCCAACGAAGGGGTTAAACACCTCTGCCTGTTC
ATCTGGCTCTCCATGAATGTCCTGCTTTTCTGGAAAACCTTCTTGCTGTATAACCAAGGG
CCAGAGTATCACTACCTCCACCAGATGTTGGGGCTAGGATTGTGTCTAAGCAGAGCCTCA
GCATCTGTTCTTAACCTCAACTGCAGCCTTATCCTTTTACCCATGTGCCGAACACTCTTG
GCTTACCTCCGAGGATCACAGAAGGTTCCAAGCAGGAGAACCAGGAGATTGTTGGATAAA
AGCAGAACATTCCATATTACCTGTGGTGTTACTATCTGTATTTTCTCAGGCGTGCATGTG
GCTGCCCATCTGGTGAATGCCCTCAACTTCTCAGTGAATTACAGTGAAGACTTTGTTGAA
CTGAATGCAGCAAGATACCGAGATGAGGATCCTAGAAAACTTCTCTTCACAACTGTTCCT
GGCCTGACAGGGGTCTGCATGGTGGTGGTGCTATTCCTCATGATCACAGCCTCTACATAT
GCAATAAGAGTTTCTAACTATGATATCTTCTGGTATACTCATAACCTCTTCTTTGTCTTC
TACATGCTGCTGACGTTGCATGTTTCAGGAGGGCTGCTGAAGTATCAAACTAATTTAGAT
ACCCACCCTCCCGGCTGCATCAGTCTTAACCGAACCAGCTCTCAGAATATTTCCTTACCA
GAGTATTTCTCAGAACATTTTCATGAACCTTTCCCTGAAGGATTTTCAAAACCGGCAGAG
TTTACCCAGCACAAATTTGTGAAGATTTGTATGGAAGAGCCCAGATTCCAAGCTAATTTT
CCACAGACTTGGCTTTGGATTTCTGGACCTTTGTGCCTGTACTGTGCCGAAAGACTTTAC
AGGTATATCCGGAGCAATAAGCCAGTCACCATCATTTCGGTCATAAGTCATCCCTCAGAT
GTCATGGAAATCCGAATGGTCAAAGAAAATTTTAAAGCAAGACCTGGTCAGTATATTACT
CTACATTGTCCCAGTGTATCTGCATTAGAAAATCATCCATTTACCCTCACAATGTGTCCA
ACTGAAACCAAAGCAACATTTGGGGTTCATCTTAAAATAGTAGGAGACTGGACAGAACGA
TTTCGAGATTTACTACTGCCTCCATCTAGTCAAGACTCCGAAATTCTGCCCTTCATTCAA
TCTAGAAATTATCCCAAGGATGACTGGAAACCATACAAGCTTAGAAGACTATACTTTATT
TGGGTATGCAGAGATATCCAGTCCTTCCGTTGGTTTGCAGATTTACTCTGTATGTTGCAT
AACAAGTTTTGGCAAGAGAACAGACCTGACTATGTCAACATCCAGCTGTACCTCAGTCAA
ACAGATGGGATACAGAAGATAATTGGAGAAAAATATCATGCACTGAATTCAAGACTGTTT
ATAGGACGTCCTCGGTGGAAACTTTTGTTTGATGAAATAGCAAAATATAACAGAGGAAAA
ACAGTTGGTGTTTTCTGTTGTGGACCCAATTCACTATCCAAGACTCTTCATAAACTGAGT
AACCAGAACAACTCATATGGGACAAGATTTGAATACAATAAAGAGTCTTTCAGCTGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC IDHGNC:7891
Chromosome Location11
LocusNone
References
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  2. Cheng G, Cao Z, Xu X, van Meir EG, Lambeth JD: Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene. 2001 May 16;269(1-2):131-40.[11376945 ]
  3. Shiose A, Kuroda J, Tsuruya K, Hirai M, Hirakata H, Naito S, Hattori M, Sakaki Y, Sumimoto H: A novel superoxide-producing NAD(P)H oxidase in kidney. J Biol Chem. 2001 Jan 12;276(2):1417-23.[11032835 ]
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  8. Park HS, Jung HY, Park EY, Kim J, Lee WJ, Bae YS: Cutting edge: direct interaction of TLR4 with NAD(P)H oxidase 4 isozyme is essential for lipopolysaccharide-induced production of reactive oxygen species and activation of NF-kappa B. J Immunol. 2004 Sep 15;173(6):3589-93.[15356101 ]
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Related FRC

FRCD ID Name Exact Mass Structure




F05238



Methylamine


31.058




F05241



Norepinephrine


169.18




F05242



L-Cysteine


121.154




F05247



Trimethylamine


59.112




F05250



Spermine


202.346




F05253



Putrescine


88.154




F05267



Decanal


156.269




F05270



2-Octenal


126.199




F05272



Heptanal


114.188




F05276



Nonanal


142.242




F05235



Dimethylamine


45.085




F05237



Hypoxanthine


136.114




F05246



Neopterin


253.218




F05251



Spermidine


145.25




F05252



Niacinamide


122.127




F05254



Methylguanidine


73.099




F05256



Guanidine


59.072




F05261



Pentosidine


378.433




F05264



4-Hydroxynonenal


156.225




F05265



Hexanal


100.161




F05266



Malondialdehyde


72.063




F05269



Ethylamine


45.085




F05271



2-Nonenal


140.226




F05273



2-Hexenal


98.145




F05275



4-Decenal


154.253