NAD-dependent protein deacylase sirtuin-5, mitochondrial

NameNAD-dependent protein deacylase sirtuin-5, mitochondrial
Synonyms3.5.1.- Regulatory protein SIR2 homolog 5 SIR2-like protein 5 SIR2L5
Gene NameSIRT5
OrganismHuman
Amino acid sequence
>lcl|BSEQ0006560|NAD-dependent protein deacylase sirtuin-5, mitochondrial
MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAG
VSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRA
IAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPI
CPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELA
HCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEA
LACHENETVS
Number of residues310
Molecular Weight33880.605
Theoretical pI8.55
GO Classification
Functions
    NAD+ ADP-ribosyltransferase activity
protein-succinyllysine desuccinylase activity
NAD+ binding
zinc ion binding
protein-glutaryllysine deglutarylase activity
protein-malonyllysine demalonylase activity
Processes
    chromatin silencing
negative regulation of cardiac muscle cell apoptotic process
peptidyl-lysine deglutarylation
peptidyl-lysine demalonylation
response to nutrient levels
peptidyl-lysine desuccinylation
protein deglutarylation
protein deacetylation
protein demalonylation
protein ADP-ribosylation
protein desuccinylation
negative regulation of reactive oxygen species metabolic process
regulation of ketone biosynthetic process
Components
    cytosol
mitochondrial intermembrane space
mitochondrion
nucleus
mitochondrial inner membrane
mitochondrial matrix
General FunctionZinc ion binding
Specific FunctionNAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins (PubMed:21908771, PubMed:22076378, PubMed:24703693). Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting (PubMed:22076378, PubMed:24703693). Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species (PubMed:24140062). Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDQ9NXA8
UniProtKB Entry NameSIR5_HUMAN
Cellular LocationMitochondrion matrix
Gene sequence
>lcl|BSEQ0019415|NAD-dependent protein deacylase sirtuin-5, mitochondrial (SIRT5)
ATGCGACCTCTCCAGATTGTCCCAAGTCGATTGATTTCCCAGCTATATTGTGGCCTGAAG
CCTCCAGCGTCCACACGAAACCAGATTTGCCTGAAAATGGCTCGGCCAAGTTCAAGTATG
GCAGATTTTCGAAAGTTTTTTGCAAAAGCAAAGCACATAGTCATCATCTCAGGAGCTGGT
GTTAGTGCAGAAAGTGGTGTTCCGACCTTCAGAGGAGCTGGAGGTTATTGGAGAAAATGG
CAAGCCCAGGACCTGGCGACTCCCCTGGCCTTTGCCCACAACCCGTCCCGGGTGTGGGAG
TTCTACCACTACCGGCGGGAGGTCATGGGGAGCAAGGAGCCCAACGCCGGGCACCGCGCC
ATAGCCGAGTGTGAGACCCGGCTGGGCAAGCAGGGCCGGCGAGTCGTGGTCATCACCCAG
AACATCGATGAGCTGCACCGCAAGGCTGGCACCAAGAACCTTCTGGAGATCCATGGTAGC
TTATTTAAAACTCGATGTACCTCTTGTGGAGTTGTGGCTGAGAATTACAAGAGTCCAATT
TGTCCAGCTTTATCAGGAAAAGGGTGTGAAGAGGCAGGCTGCGGGGGCTTGCTGCGACCT
CACGTCGTGTGGTTTGGAGAAAACCTGGATCCTGCCATTCTGGAGGAGGTTGACAGAGAG
CTCGCCCACTGTGATTTATGTCTAGTGGTGGGCACTTCCTCTGTGGTGTACCCAGCAGCC
ATGTTTGCCCCCCAGGTGGCTGCCAGGGGCGTGCCAGTGGCTGAATTTAACACGGAGACC
ACCCCAGCTACGAACAGATTCAGGTTTCATTTCCAGGGACCCTGTGGAACGACTCTTCCT
GAAGCCCTTGCCTGTCATGAAAATGAAACTGTTTCTTAA
GenBank Gene IDAF083110
GeneCard IDNone
GenAtlas IDSIRT5
HGNC IDHGNC:14933
Chromosome Location6
Locus6p23
References
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Related FRC

FRCD ID Name Exact Mass Structure




F05252



Niacinamide


122.127