FRCD

Lysine-specific demethylase 3A

Name	Lysine-specific demethylase 3A
Synonyms	1.14.11.- JHDM2A JmjC domain-containing histone demethylation protein 2A JMJD1 JMJD1A Jumonji domain-containing protein 1A KIAA0742 TSGA
Gene Name	KDM3A
Organism	Human
Amino acid sequence	>lcl\|BSEQ0008940\|Lysine-specific demethylase 3A MVLTLGESWPVLVGRRFLSLSAADGSDGSHDSWDVERVAEWPWLSGTIRAVSHTDVTKKD LKVCVEFDGESWRKRRWIEVYSLLRRAFLVEHNLVLAERKSPEISERIVQWPAITYKPLL DKAGLGSITSVRFLGDQQRVFLSKDLLKPIQDVNSLRLSLTDNQIVSKEFQALIVKHLDE SHLLKGDKNLVGSEVKIYSLDPSTQWFSATVINGNPASKTLQVNCEEIPALKIVDPSLIH VEVVHDNLVTCGNSARIGAVKRKSSENNGTLVSKQAKSCSEASPSMCPVQSVPTTVFKEI LLGCTAATPPSKDPRQQSTPQAANSPPNLGAKIPQGCHKQSLPEEISSCLNTKSEALRTK PDVCKAGLLSKSSQIGTGDLKILTEPKGSCTQPKTNTDQENRLESVPQALTGLPKECLPT KASSKAELEIANPPELQKHLEHAPSPSDVSNAPEVKAGVNSDSPNNCSGKKVEPSALACR SQNLKESSVKVDNESCCSRSNNKIQNAPSRKSVLTDPAKLKKLQQSGEAFVQDDSCVNIV AQLPKCRECRLDSLRKDKEQQKDSPVFCRFFHFRRLQFNKHGVLRVEGFLTPNKYDNEAI GLWLPLTKNVVGIDLDTAKYILANIGDHFCQMVISEKEAMSTIEPHRQVAWKRAVKGVRE MCDVCDTTIFNLHWVCPRCGFGVCVDCYRMKRKNCQQGAAYKTFSWLKCVKSQIHEPENL MPTQIIPGKALYDVGDIVHSVRAKWGIKANCPCSNRQFKLFSKPASKEDLKQTSLAGEKP TLGAVLQQNPSVLEPAAVGGEAASKPAGSMKPACPASTSPLNWLADLTSGNVNKENKEKQ PTMPILKNEIKCLPPLPPLSKSSTVLHTFNSTILTPVSNNNSGFLRNLLNSSTGKTENGL KNTPKILDDIFASLVQNKTTSDLSKRPQGLTIKPSILGFDTPHYWLCDNRLLCLQDPNNK SNWNVFRECWKQGQPVMVSGVHHKLNSELWKPESFRKEFGEQEVDLVNCRTNEIITGATV GDFWDGFEDVPNRLKNEKEPMVLKLKDWPPGEDFRDMMPSRFDDLMANIPLPEYTRRDGK LNLASRLPNYFVRPDLGPKMYNAYGLITPEDRKYGTTNLHLDVSDAANVMVYVGIPKGQC EQEEEVLKTIQDGDSDELTIKRFIEGKEKPGALWHIYAAKDTEKIREFLKKVSEEQGQEN PADHDPIHDQSWYLDRSLRKRLHQEYGVQGWAIVQFLGDVVFIPAGAPHQVHNLYSCIKV AEDFVSPEHVKHCFWLTQEFRYLSQTHTNHEDKLQVKNVIYHAVKDAVAMLKASESSFGK P
Number of residues	1321
Molecular Weight	147339.98
Theoretical pI	None
GO Classification	Functions dioxygenase activity transcription factor activity, sequence-specific DNA binding androgen receptor binding iron ion binding core promoter binding transcription regulatory region sequence-specific DNA binding Processes positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter formaldehyde biosynthetic process transcription, DNA-templated histone H3-K9 demethylation hormone-mediated signaling pathway histone H3-K9 dimethylation spermatid nucleus elongation negative regulation of histone H3-K9 methylation androgen receptor signaling pathway chromatin organization Components cytoplasm membrane nucleus nucleoplasm
General Function	Transcription regulatory region sequence-specific dna binding
Specific Function	Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
Transmembrane Regions
GenBank Protein ID
UniProtKB ID	Q9Y4C1
UniProtKB Entry Name	KDM3A_HUMAN
Cellular Location	Cytoplasm
Gene sequence	>lcl\|BSEQ0013520\|Lysine-specific demethylase 3A (KDM3A) ATGGTGCTCACGCTCGGAGAAAGTTGGCCGGTATTGGTGGGGAGGAGGTTTCTCAGTCTG TCCGCAGCCGACGGCAGCGATGGCAGCCACGACAGCTGGGACGTGGAGCGCGTCGCCGAG TGGCCCTGGCTCTCCGGGACCATTCGAGCTGTTTCCCACACCGACGTTACCAAGAAGGAT CTGAAGGTGTGTGTGGAATTTGATGGGGAATCTTGGAGGAAAAGAAGATGGATAGAAGTC TACAGCCTTCTAAGGAGAGCATTTTTAGTAGAACATAATTTGGTTTTAGCTGAACGAAAG TCACCTGAAATTTCTGAACGAATTGTACAGTGGCCTGCAATAACGTACAAACCTCTGTTG GACAAAGCTGGTTTGGGATCCATAACTTCTGTTCGCTTTCTGGGAGATCAACAAAGAGTA TTTCTTTCTAAAGACCTTTTGAAGCCTATACAGGATGTAAACAGTCTTCGACTTTCTCTT ACGGATAATCAGATTGTCAGTAAAGAATTTCAAGCTTTGATTGTGAAGCATTTAGATGAA AGCCATCTTTTAAAAGGTGACAAAAACTTAGTTGGTTCAGAAGTAAAAATTTATAGCTTG GACCCATCTACTCAGTGGTTTTCAGCAACCGTTATAAATGGAAACCCAGCATCAAAAACT CTTCAAGTCAACTGTGAGGAGATTCCAGCACTGAAAATTGTTGATCCGTCACTGATTCAT GTTGAAGTTGTACACGATAACCTTGTGACATGTGGTAATTCTGCAAGAATTGGAGCTGTA AAACGCAAGTCTTCTGAGAATAATGGAACCCTGGTTTCCAAACAAGCAAAATCTTGCTCT GAGGCCTCTCCCAGTATGTGTCCTGTGCAGTCTGTACCTACAACAGTTTTTAAGGAGATA CTGCTTGGCTGTACTGCGGCAACTCCACCTAGTAAGGACCCAAGACAGCAAAGTACTCCC CAGGCTGCCAACTCTCCACCTAACCTTGGAGCAAAAATTCCTCAAGGATGTCATAAACAA AGTTTACCAGAGGAAATTTCTTCCTGTCTAAATACAAAGTCTGAAGCTCTGAGAACAAAA CCAGATGTCTGCAAAGCAGGGTTGCTCTCAAAGTCCTCTCAGATTGGAACTGGAGACTTG AAAATTCTGACTGAGCCAAAAGGCAGCTGTACTCAGCCTAAGACAAACACTGATCAGGAA AACAGATTGGAGTCTGTTCCACAAGCATTGACTGGCCTTCCTAAGGAGTGCTTACCTACA AAGGCTTCTTCTAAGGCAGAATTGGAAATTGCCAATCCTCCTGAACTGCAGAAGCACCTA GAACATGCACCTTCCCCATCGGATGTTTCAAATGCACCAGAAGTGAAAGCAGGTGTCAAT AGTGATAGCCCTAATAACTGTTCAGGAAAAAAGGTAGAACCTTCAGCTTTAGCTTGCCGA TCACAGAATTTAAAGGAATCTTCAGTAAAAGTAGATAATGAAAGCTGTTGTTCAAGAAGC AACAATAAAATCCAGAATGCCCCATCCAGGAAGTCGGTTTTGACAGACCCAGCTAAACTC AAAAAGCTGCAACAGAGTGGCGAGGCCTTCGTACAGGATGATTCTTGTGTGAACATCGTG GCACAGTTGCCTAAATGCCGAGAGTGTCGCTTGGACAGTCTCCGCAAGGATAAGGAGCAA CAGAAGGACTCACCTGTGTTTTGCCGCTTCTTTCACTTCAGGAGGTTACAATTCAACAAA CATGGTGTGTTGCGGGTAGAAGGCTTCTTAACACCAAACAAGTATGACAATGAAGCAATT GGCTTGTGGTTACCTTTAACCAAAAACGTTGTGGGGATTGATTTGGACACAGCAAAGTAC ATCTTGGCCAACATTGGAGACCACTTCTGTCAAATGGTGATTTCTGAAAAGGAAGCTATG TCAACTATTGAGCCACACAGACAGGTTGCTTGGAAGCGAGCTGTCAAAGGTGTTCGAGAA ATGTGTGATGTGTGCGACACCACCATCTTCAACCTGCACTGGGTGTGTCCTCGGTGTGGG TTTGGAGTATGTGTGGACTGCTACCGGATGAAGAGAAAGAATTGCCAACAGGGTGCTGCT TACAAGACTTTCTCTTGGCTAAAATGTGTGAAGAGTCAGATACATGAACCAGAGAACTTA ATGCCCACACAGATCATTCCTGGAAAAGCACTCTATGATGTTGGAGACATTGTTCATTCT GTAAGAGCGAAATGGGGAATAAAGGCAAACTGCCCTTGTTCAAACAGGCAATTCAAACTC TTTTCAAAGCCAGCCTCAAAGGAAGACCTAAAACAGACTTCTTTAGCTGGAGAAAAACCG ACTCTTGGTGCAGTGCTCCAGCAGAATCCCTCAGTGTTGGAGCCAGCAGCTGTGGGTGGG GAAGCAGCCTCCAAGCCAGCCGGCAGCATGAAGCCTGCCTGTCCAGCCAGCACATCTCCT CTAAACTGGCTGGCCGACCTAACCAGCGGGAATGTCAACAAGGAAAACAAGGAAAAACAA CCAACAATGCCAATTTTAAAGAATGAAATCAAATGCCTTCCACCCCTCCCACCTTTAAGC AAATCCAGCACAGTCCTCCATACGTTTAACAGCACAATTTTGACACCCGTAAGCAACAAC AATTCTGGTTTCCTCCGGAATCTCTTGAATTCTTCTACAGGAAAGACAGAAAATGGACTC AAGAATACACCAAAAATCCTTGATGACATCTTTGCCTCTTTGGTGCAAAATAAGACGACT TCTGATTTATCTAAGAGGCCTCAAGGACTAACCATCAAGCCCAGCATTCTGGGCTTTGAC ACTCCTCACTATTGGCTTTGTGATAATCGCTTGCTGTGCTTGCAAGACCCCAACAATAAG AGCAACTGGAATGTGTTTAGGGAGTGCTGGAAACAAGGGCAGCCAGTGATGGTGTCTGGA GTGCATCATAAATTGAACTCTGAACTTTGGAAACCTGAATCCTTCAGGAAAGAGTTTGGT GAGCAGGAAGTAGACCTAGTTAATTGTAGGACCAATGAAATCATCACAGGAGCCACAGTA GGAGACTTCTGGGATGGATTTGAAGATGTTCCAAATCGTTTGAAAAATGAAAAAGAACCA ATGGTGTTGAAACTTAAGGACTGGCCACCAGGAGAAGATTTTAGAGATATGATGCCTTCC AGGTTTGATGATCTGATGGCCAACATTCCACTGCCCGAGTACACAAGGCGAGATGGCAAA CTGAATTTGGCCTCTAGGCTGCCAAACTACTTTGTTCGGCCAGATCTGGGCCCCAAGATG TATAATGCTTATGGATTAATCACTCCTGAAGATCGGAAATATGGAACAACAAATCTTCAC TTAGATGTATCTGATGCAGCTAATGTCATGGTCTATGTGGGAATTCCCAAAGGACAGTGT GAGCAAGAAGAAGAAGTCCTTAAGACCATCCAAGATGGAGATTCTGACGAACTCACAATA AAGCGATTTATTGAAGGAAAAGAGAAGCCAGGAGCACTGTGGCACATATATGCTGCAAAG GACACGGAGAAGATAAGGGAATTTCTTAAAAAGGTATCAGAAGAGCAAGGTCAAGAAAAC CCAGCAGACCACGATCCTATTCATGATCAAAGCTGGTATTTAGACCGATCATTAAGAAAA CGTCTTCATCAAGAGTATGGAGTTCAAGGCTGGGCTATTGTACAGTTTCTTGGGGATGTG GTGTTTATCCCGGCAGGAGCTCCACATCAGGTTCATAACTTATATAGCTGCATCAAAGTG GCTGAAGATTTTGTTTCTCCAGAGCATGTTAAACACTGCTTCTGGCTTACTCAGGAATTC CGATATCTGTCACAGACTCATACCAATCACGAAGATAAATTACAGGTGAAGAATGTTATC TACCATGCAGTGAAAGATGCAGTTGCTATGCTGAAAGCCAGTGAATCCAGTTTTGGCAAA CCTTAA
GenBank Gene ID
GeneCard ID	None
GenAtlas ID
HGNC ID	HGNC:20815
Chromosome Location	2
Locus	None
References	Nagase T, Ishikawa K, Suyama M, Kikuno R, Miyajima N, Tanaka A, Kotani H, Nomura N, Ohara O: Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 1998 Oct 30;5(5):277-86.[9872452 ] Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Blocker H, Heubner D, Hoerlein A, Michel G, Wedler H, Kohrer K, Ottenwalder B, Poustka A, Wiemann S, Schupp I: The full-ORF clone resource of the German cDNA Consortium. BMC Genomics. 2007 Oct 31;8:399.[17974005 ] Hillier LW, Graves TA, Fulton RS, Fulton LA, Pepin KH, Minx P, Wagner-McPherson C, Layman D, Wylie K, Sekhon M, Becker MC, Fewell GA, Delehaunty KD, Miner TL, Nash WE, Kremitzki C, Oddy L, Du H, Sun H, Bradshaw-Cordum H, Ali J, Carter J, Cordes M, Harris A, Isak A, van Brunt A, Nguyen C, Du F, Courtney L, Kalicki J, Ozersky P, Abbott S, Armstrong J, Belter EA, Caruso L, Cedroni M, Cotton M, Davidson T, Desai A, Elliott G, Erb T, Fronick C, Gaige T, Haakenson W, Haglund K, Holmes A, Harkins R, Kim K, Kruchowski SS, Strong CM, Grewal N, Goyea E, Hou S, Levy A, Martinka S, Mead K, McLellan MD, Meyer R, Randall-Maher J, Tomlinson C, Dauphin-Kohlberg S, Kozlowicz-Reilly A, Shah N, Swearengen-Shahid S, Snider J, Strong JT, Thompson J, Yoakum M, Leonard S, Pearman C, Trani L, Radionenko M, Waligorski JE, Wang C, Rock SM, Tin-Wollam AM, Maupin R, Latreille P, Wendl MC, Yang SP, Pohl C, Wallis JW, Spieth J, Bieri TA, Berkowicz N, Nelson JO, Osborne J, Ding L, Meyer R, Sabo A, Shotland Y, Sinha P, Wohldmann PE, Cook LL, Hickenbotham MT, Eldred J, Williams D, Jones TA, She X, Ciccarelli FD, Izaurralde E, Taylor J, Schmutz J, Myers RM, Cox DR, Huang X, McPherson JD, Mardis ER, Clifton SW, Warren WC, Chinwalla AT, Eddy SR, Marra MA, Ovcharenko I, Furey TS, Miller W, Eichler EE, Bork P, Suyama M, Torrents D, Waterston RH, Wilson RK: Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Nature. 2005 Apr 7;434(7034):724-31.[15815621 ] Yamane K, Toumazou C, Tsukada Y, Erdjument-Bromage H, Tempst P, Wong J, Zhang Y: JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. Cell. 2006 May 5;125(3):483-95. Epub 2006 Apr 6.[16603237 ] Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP: A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10762-7. doi: 10.1073/pnas.0805139105. Epub 2008 Jul 31.[18669648 ] Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M: Lysine acetylation targets protein complexes and co-regulates major cellular functions. Science. 2009 Aug 14;325(5942):834-40. doi: 10.1126/science.1175371. Epub 2009 Jul 16.[19608861 ] Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal. 2010 Jan 12;3(104):ra3. doi: 10.1126/scisignal.2000475.[20068231 ] Rigbolt KT, Prokhorova TA, Akimov V, Henningsen J, Johansen PT, Kratchmarova I, Kassem M, Mann M, Olsen JV, Blagoev B: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal. 2011 Mar 15;4(164):rs3. doi: 10.1126/scisignal.2001570.[21406692 ] Sjoblom T, Jones S, Wood LD, Parsons DW, Lin J, Barber TD, Mandelker D, Leary RJ, Ptak J, Silliman N, Szabo S, Buckhaults P, Farrell C, Meeh P, Markowitz SD, Willis J, Dawson D, Willson JK, Gazdar AF, Hartigan J, Wu L, Liu C, Parmigiani G, Park BH, Bachman KE, Papadopoulos N, Vogelstein B, Kinzler KW, Velculescu VE: The consensus coding sequences of human breast and colorectal cancers. Science. 2006 Oct 13;314(5797):268-74. Epub 2006 Sep 7.[16959974 ]

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FRCD ID	Name	Exact Mass
F03310	Nickel	58.693
F04252	Nickelocene	188.883
F02409	Nickel chloride	129.593

FRCD

Lysine-specific demethylase 3A

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