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Nature Methods: Focus on Synthetic Biology

来源: 时间:七月 21, 2014, 10:31 a.m.

http://www.nature.com/nmeth/focus/syntheticbiology/index.html


FOCUS

Focus on Synthetic Biology

Synthetic biology spans many disciplines and has many different goals. A Commentary discusses its role in addressing fundamental questions such as the origin of life. Although recent advances in the field have brought us closer to some of synthetic biology's ambitious aims, there is still a need for better characterization of basic building blocks. One Review looks at advances and challenges in DNA synthesis and error correction, a second Review discusses optimal genetic circuit design, and a research paper presents a computational tool for efficient assembly of genetic parts. A Perspective highlights advances in whole-genome assembly and activation. Well-characterized tools and methods will allow the field to move from costly trial-and-error approaches to a more standardized plug-and-play approach.

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EDITORIAL

Focus on Synthetic Biology

Synthetic Biology: Back to the Basics p463

Nicole Rusk

doi:10.1038/nmeth.2941

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COMMENTARY

Focus on Synthetic Biology

A synthetic approach to abiogenesis pp495 - 498

James Attwater & Philipp Holliger

doi:10.1038/nmeth.2893

Authors discuss how synthetic biology approaches could be applied to assemble synthetic quasibiological systems able to replicate and evolve, illuminating universal properties of life and the search for its origins.

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REVIEWS

Focus on Synthetic Biology

Large-scale de novo DNA synthesis: technologies and applications pp499 - 507

Sriram Kosuri & George M Church

doi:10.1038/nmeth.2918

This Review discusses large de novo DNA synthesis via oligos or arrays, describes gene assembly and error correction and considers applications for large-scale DNA synthesis.

Focus on Synthetic Biology

Principles of Genetic Circuit Design pp508 - 520

Jennifer A N Brophy & Christopher A Voigt

doi:10.1038/nmeth.2926

This Review introduces tools to build transcriptional circuits and explains how the choice of different tools can affect circuit behavior and how its operation can be affected by the cellular host.

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PERSPECTIVE

Focus on Synthetic Biology

Programming biological operating systems: genome design, assembly and activation pp521 - 526

Daniel G Gibson

doi:10.1038/nmeth.2894

This Perspective takes the reader through the important steps in bacterial genome assembly and activation and concludes with an outlook on how customized genomes may be achieved.

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ARTICLE

Focus on Synthetic Biology

Interactive assembly algorithms for molecular cloning

Evan Appleton, Jenhan Tao, Traci Haddock & Douglas Densmore

doi:10.1038/nmeth.2939

Raven calculates assembly plans for complex genetic constructs from thousands of parts. It integrates user feedback on failed intermediate assemblies to improve the final outcome.



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