Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1

NameCarboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
Synonyms
  • 3.1.3.16
  • NIF3
  • NLI-IF
  • NLI-interacting factor 3
  • NLIIF
  • Nuclear LIM interactor-interacting factor 3
  • SCP1
  • Small C-terminal domain phosphatase 1
  • Small CTD phosphatase 1
Gene NameCTDSP1
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009472|Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
MDSSAVITQISKEEARGPLRGKGDQKSAASQKPRSRGILHSLFCCVCRDDGEALPAHSGA
PLLVEENGAIPKQTPVQYLLPEAKAQDSDKICVVIDLDETLVHSSFKPVNNADFIIPVEI
DGVVHQVYVLKRPHVDEFLQRMGELFECVLFTASLAKYADPVADLLDKWGAFRARLFRES
CVFHRGNYVKDLSRLGRDLRRVLILDNSPASYVFHPDNAVPVASWFDNMSDTELHDLLPF
FEQLSRVDDVYSVLRQPRPGS
Number of residuesNone
Molecular Weight29202.94
Theoretical pINone
GO Classification
Functions
  • metal ion binding
  • CTD phosphatase activity
Processes
  • protein dephosphorylation
  • regulation of transcription from RNA polymerase II promoter
  • negative regulation of neuron differentiation
  • negative regulation of protein phosphorylation
  • negative regulation of G1/S transition of mitotic cell cycle
Components
  • nucleus
  • extracellular exosome
  • nucleoplasm
General FunctionMetal ion binding
Specific FunctionPreferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDQ9GZU7
UniProtKB Entry Name
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0017469|Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (CTDSP1)
ATGGTGGCCGCCCCGTGGGCTACCCAGGAGCAGGAGGAGGGCCGAGGGATCCAGCCCGGG
GACCGGGGTGACCAGAAGTCAGCAGCTTCCCAGAAGCCCCGAAGCCGGGGCATCCTCCAC
TCACTCTTCTGCTGTGTCTGCCGGGATGATGGGGAGGCCCTGCCTGCTCACAGCGGGGCG
CCCCTGCTTGTGGAGGAGAATGGCGCCATCCCTAAGACCCCAGTCCAATACCTGCTCCCT
GAGGCCAAGGCCCAGGACTCAGACAAGATCTGCGTGGTCATCGACCTGGACGAGACCCTG
GTGCACAGCTCCTTCAAGCCAGTGAACAACGCGGACTTCATCATCCCTGTGGAGATTGAT
GGGGTGGTCCACCAGGTCTACGTGTTGAAGCGTCCTCACGTGGATGAGTTCCTGCAGCGA
ATGGGCGAGCTCTTTGAATGTGTGCTGTTCACTGCTAGCCTCGCCAAGTACGCAGACCCA
GTAGCTGACCTGCTGGACAAATGGGGGGCCTTCCGGGCCCGGCTGTTTCGAGAGTCCTGC
GTCTTCCACCGGGGGAACTACGTGAAGGACCTGAGCCGGTTGGGTCGAGACCTGCGGCGG
GTGCTCATCCTGGACAATTCACCTGCCTCCTATGTCTTCCATCCAGACAATGCTGTACCG
GTGGCCTCGTGGTTTGACAACATGAGTGACACAGAGCTCCACGACCTCCTCCCCTTCTTC
GAGCAACTCAGCCGTGTGGACGACGTGTACTCAGTGCTCAGGCAGCCACGGCCAGGGAGC
TAG
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome Location2
LocusNone
References
  1. Marquet S, Lepage P, Hudson TJ, Musser JM, Schurr E: Complete nucleotide sequence and genomic structure of the human NRAMP1 gene region on chromosome region 2q35. Mamm Genome. 2000 Sep;11(9):755-62. [10967134 ]
  2. Yeo M, Lin PS, Dahmus ME, Gill GN: A novel RNA polymerase II C-terminal domain phosphatase that preferentially dephosphorylates serine 5. J Biol Chem. 2003 Jul 11;278(28):26078-85. Epub 2003 Apr 28. [12721286 ]
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  5. Yeo M, Lee SK, Lee B, Ruiz EC, Pfaff SL, Gill GN: Small CTD phosphatases function in silencing neuronal gene expression. Science. 2005 Jan 28;307(5709):596-600. [15681389 ]
  6. Van Damme P, Lasa M, Polevoda B, Gazquez C, Elosegui-Artola A, Kim DS, De Juan-Pardo E, Demeyer K, Hole K, Larrea E, Timmerman E, Prieto J, Arnesen T, Sherman F, Gevaert K, Aldabe R: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB. Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12449-54. doi: 10.1073/pnas.1210303109. Epub 2012 Jul 18. [22814378 ]
  7. Kamenski T, Heilmeier S, Meinhart A, Cramer P: Structure and mechanism of RNA polymerase II CTD phosphatases. Mol Cell. 2004 Aug 13;15(3):399-407. [15304220 ]
  8. Zhang Y, Kim Y, Genoud N, Gao J, Kelly JW, Pfaff SL, Gill GN, Dixon JE, Noel JP: Determinants for dephosphorylation of the RNA polymerase II C-terminal domain by Scp1. Mol Cell. 2006 Dec 8;24(5):759-70. [17157258 ]

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