RNA 3'-terminal phosphate cyclase

NameRNA 3'-terminal phosphate cyclase
Synonyms
  • 6.5.1.4
  • RNA cyclase
  • yhgJ
  • yhgK
Gene NamertcA
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0006359|RNA 3'-terminal phosphate cyclase
MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAKPGLLRQHLTAVKAATE
ICGATVEGAELGSQRLLFRPGTVRGGDYRFAIGSAGSCTLVLQTVLPALWFADGPSRVEV
SGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFYPAGGGVVATEVSPVASFNTL
QLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSLHEQNIHNLPRDQGPGNTVSLEV
ESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLASTAAVGEYLADQLVLPMALAGAGEF
TVAHPSCHLLTNIAVVERFLPVRFSLIETDGVTRVSIE
Number of residuesNone
Molecular Weight35902.925
Theoretical pI7.26
GO Classification
Functions
  • ATP binding
  • RNA binding
  • RNA-3'-phosphate cyclase activity
Processes
  • RNA processing
Components
  • cytoplasm
General FunctionRna-3'-phosphate cyclase activity
Specific FunctionCatalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP46849
UniProtKB Entry Name
Cellular LocationCytoplasm
Gene sequence
>lcl|BSEQ0012274|RNA 3'-terminal phosphate cyclase (rtcA)
ATGAAAAGGATGATTGCGCTGGATGGCGCACAGGGCGAAGGTGGCGGGCAGATCCTGCGC
TCGGCGCTGAGCCTGTCGATGATAACCGGCCAGCCATTTACCATCACCAGCATTCGTGCC
GGGCGGGCGAAACCGGGGCTGTTGCGCCAGCATCTGACCGCGGTAAAGGCGGCGACGGAA
ATTTGCGGAGCAACCGTGGAAGGCGCGGAGCTGGGGTCGCAGCGTCTGCTCTTCCGGCCC
GGCACCGTGCGCGGCGGCGATTACCGCTTTGCTATCGGTAGCGCCGGAAGTTGTACGCTG
GTGCTGCAAACGGTGCTGCCCGCGCTGTGGTTTGCCGATGGACCTTCGCGTGTTGAAGTG
AGCGGCGGCACCGATAACCCGTCGGCGCCGCCTGCGGATTTTATCCGCCGGGTGCTGGAG
CCGCTGCTGGCGAAAATAGGAATTCATCAGCAAACCACGCTGTTACGCCACGGTTTTTAC
CCTGCCGGAGGCGGTGTGGTGGCAACGGAAGTCTCGCCCGTGGCATCGTTTAACACCTTG
CAACTTGGCGAGCGCGGGAACATTGTGCAGATGCGTGGAGAAGTTCTATTAGCTGGTGTG
CCGCGCCATGTTGCTGAGCGTGAAATCGCTACACTGGCGGGGAGTTTTTCCCTGCATGAA
CAGAATATTCATAACCTGCCGCGCGACCAGGGGCCGGGTAATACCGTCTCGCTTGAAGTC
GAAAGTGAAAATATCACCGAACGCTTTTTTGTCGTCGGTGAAAAGCGCGTCAGTGCCGAG
GTGGTTGCGGCACAGTTGGTGAAAGAGGTGAAACGCTACCTGGCAAGCACGGCGGCGGTG
GGGGAATATCTTGCTGACCAACTGGTGCTACCGATGGCGCTGGCGGGCGCGGGGGAATTT
ACGGTCGCCCATCCCTCATGCCATCTGCTGACCAATATCGCGGTGGTGGAGCGTTTCTTG
CCGGTGCGGTTTAGTTTGATAGAAACAGATGGCGTAACGCGGGTGAGCATTGAATGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [9278503 ]
  2. Riley M, Abe T, Arnaud MB, Berlyn MK, Blattner FR, Chaudhuri RR, Glasner JD, Horiuchi T, Keseler IM, Kosuge T, Mori H, Perna NT, Plunkett G 3rd, Rudd KE, Serres MH, Thomas GH, Thomson NR, Wishart D, Wanner BL: Escherichia coli K-12: a cooperatively developed annotation snapshot--2005. Nucleic Acids Res. 2006 Jan 5;34(1):1-9. Print 2006. [16397293 ]
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [16738553 ]
  4. Cole ST, Raibaud O: The nucleotide sequence of the malT gene encoding the positive regulator of the Escherichia coli maltose regulon. Gene. 1986;42(2):201-8. [3015733 ]
  5. Genschik P, Billy E, Swianiewicz M, Filipowicz W: The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea. EMBO J. 1997 May 15;16(10):2955-67. [9184239 ]
  6. Genschik P, Drabikowski K, Filipowicz W: Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon. J Biol Chem. 1998 Sep 25;273(39):25516-26. [9738023 ]
  7. Palm GJ, Billy E, Filipowicz W, Wlodawer A: Crystal structure of RNA 3'-terminal phosphate cyclase, a ubiquitous enzyme with unusual topology. Structure. 2000 Jan 15;8(1):13-23. [10673421 ]
  8. Tanaka N, Smith P, Shuman S: Structure of the RNA 3'-phosphate cyclase-adenylate intermediate illuminates nucleotide specificity and covalent nucleotidyl transfer. Structure. 2010 Mar 14;18(4):449-57. doi: 10.1016/j.str.2010.01.016. [20399182 ]

From www.t3db.ca