Genome polyprotein
| Name | Genome polyprotein |
|---|---|
| Synonyms |
|
| Gene Name | None |
| Organism | Coxsackievirus A9 (strain Griggs) |
| Amino acid sequence | >lcl|BSEQ0017379|Genome polyprotein MGAQVSTQKTGAHETSLSAAGNSIIHYTNINYYKDAASNSANRQDFTQDPSKFTEPVKDV MIKSLPALNSPTVEECGYSDRVRSITLGNSTITTQECANVVVGYGRWPTYLRDDEATAED QPTQPDVATCRFYTLDSIKWEKGSVGWWWKFPEALSDMGLFGQNMQYHYLGRAGYTIHLQ CNASKFHQGCLLVVCVPEAEMGGAVVGQAFSATAMANGDKAYEFTSATQSDQTKVQTAIH NAGMGVGVGNLTIYPHQWINLRTNNSATIVMPYINSVPMDNMFRHYNFTLMVIPFVKLDY ADTASTYVPITVTVAPMCAEYNGLRLAQAQGLPTMNTPGSTQFLTSDDFQSPCALPQFDV TPSMNIPGEVKNLMEIAEVDSVVPVNNVQDTTDQMEMFRIPVTINAPLQQQVFGLRLQPG LDSVFKHTLLGEILNYYAHWSGSMKLTFVFCGSAMATGKFLIAYSPPGANPPKTRKDAML GTHIIWDIGLQSSCVLCVPWISQTHYRLVQQDEYTSAGYVTCWYQTGMIVPPGTPNSSSI MCFASACNDFSVRMLRDTPFISQDNKLQGDVEEAIERARCTVADTMRTGPSNSASVPALT AVETGHTSQVTPSDTMQTRHVKNYHSRSESTVENFLGRSACVYMEEYKTTDKHVNKKFVA WPINTKQMVQMRRKLEMFTYLRFDMEVTFVITSRQDPGTTLAQDMPVLTRQIMYVPPGGP IPAKVDDYAWQTSTNPSIFWTEGNAPARMSIPFISIGNAYSNFYDGWSNFDQRGSYGYNT LNNLGHIYVRHVSGSSPHPITSTIRVYFKPKHTRAWVPRPPRLCQYKKAFSVDFTPTPIT DTRKDINTVTTVAQSRRRGDMSTLNTHGAFGQQSGAVYVGNYRVINRHLATHTDWQNCVW EDYNRDLLVSTTTAHGCDVIARCQCTTGVYFCASKNKHYPVSFEGPGLVEVQESEYYPKR YQSHVLLAAGFSEPGDCGGILRCEHGVIGIVTMGGEGVVGFADVRDLLWLEDDAMEQGVK DYVEQLGNAFGSGFTNQICEQVNLLKESLVGQDSILEKSLKALVKIISALVIVVRNHDDL ITVTAILALIGCTSSPWRWLKQKVSQYYGIPMAERQNDSWLKKFTEMTNACKRMEWIAIK IQKFIEWLKVKILPEVREKHEFLNRLKQLPLLESQIATIEQSAPSQSDQEQLFSNVQYFA HYCRKYAPLYAAEAKRVFSLEKKMSNYIQFKSKCRIEPVCLLLHGSPGAGKSVATNLIGR SLAEKLNSSVYSLPPDPDHFDGYKQQAVVIMDDLCQNPDGKDVSLFCQMVSSVDFVPPMA ALEEKGILFTSPFVLASTNAGSINAPTVSDSRALARRFHFDMNIEVISMYSQNGKINMPM SVKTCDEECCPVNFKKCCPLVCGKAIQFIDRRTQVRYSLDMLVTEMFREYNHRHSVGATL EALFQGPPIYREIKISVAPETPPPPVIADLLKSVDSEDVREYCKEKGWLIPEVNSTLQIE KYVSRAFICLQAITTFVSVAGIIYIIYKLFAGFQGAYTGIPNQKPKVPTLRQAKVQGPAF EFAVAMMKRNSSTVKTEYGEFTMLGIYDRWAVLPRHAKPGPTILMNDQEVGVMDAKELVD KDGTNLELTLLKLNRNEKFRDIRGFLAKEEMEVNEAVLAINTSKFPNMYIPVGQVTDYGF LNLGGTPTKRMLMYNFPTRAGQCGGVLMSTGKVLGIHVGGNGHQGFSAALLKHYFNDEQG EIEFIESSKDAGFPIINTPSKTKLEPSVFHQVFEGVKEPAVLRNGDPRLKANFEEAIFSK YIGNVNTHVDEYMLEAVDHYAGQLATLDISTEPMKLEDAVYGTEGLEALDLTTSAGYPYV ALGIKKRDILSKKTRDLTKLKECMDKYGLNLPMITYVKDQLRSAEKVAKGKSRLIEASSL NDSVAMRQTFGNLYKTFHLNPGIVTGSAVGCDPDLFWSKIPVMLNGHLIAFDYSGYDASL SPVWFACLKLLLEKLGYSHKETNYIDYLCNSHHLYRDKHYFVRGGMPSGCSGTSIFNSMI NNIIIRTLMLKVYKGIDLDQFRMIAYGDDVIASYPWPIDASLLAEAGKDYGLIMTPADKG ECFNEVTWTNVTFLKRYFRADEQYPFLVHPVMPMKDIHESIRWTKDPKNTQDHVRSLCLL AWHNGEHEYEEFIRKIRSVPVGRCLTLPAFSTLRRKWLDSF |
| Number of residues | None |
| Molecular Weight | 246533.13 |
| Theoretical pI | 7.09 |
| GO Classification |
Functions
Processes
Components
|
| General Function | Structural molecule activity |
| Specific Function | Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host integrin ITGAV/ITGB6 to provide virion attachment to target host cells. This attachment induces virion internalization. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks (By similarity).Capsid protein VP2: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity).Capsid protein VP3: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity).Capsid protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm. After genome has been released, the channel shrinks (By similarity).Capsid protein VP0: Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation. Allows the capsid to remain inactive before the maturation step (By similarity).Protein 2A: Cysteine protease that cleaves viral polyprotein and specific host proteins. It is responsible for the cleavage between the P1 and P2 regions, first cleavage occurring in the polyprotein. Cleaves also the host translation initiation factor EIF4G1, in order to shut down the capped cellular mRNA translation. Inhibits the host nucleus-cytoplasm protein and RNA trafficking by cleaving host members of the nuclear pores (By similarity).Protein 2B: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cyctoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication (By similarity).Protein 2C: Induces and associates with structural rearrangements of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3 (By similarity).Protein 3AB: Localizes the viral replication complex to the surface of membranous vesicles. Together with protein 3CD binds the Cis-Active RNA Element (CRE) which is involved in RNA synthesis initiation. Acts as a cofactor to stimulate the activity of 3D polymerase, maybe through a nucleid acid chaperone activity (By similarity).Protein 3A: Localizes the viral replication complex to the surface of membranous vesicles. It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the dissassembly of the Golgi complex, possibly through GBF1 interaction. This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface (By similarity).Viral protein genome-linked: acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. VPg may be removed in the cytoplasm by an unknown enzyme termed "unlinkase". VPg is not cleaved off virion genomes because replicated genomic RNA are encapsidated at the site of replication (By similarity).Protein 3CD: Is involved in the viral replication complex and viral polypeptide maturation. It exhibits protease activity with a specificity and catalytic efficiency that is different from protease 3C. Protein 3CD lacks polymerase activity. The 3C domain in the context of protein 3CD may have an RNA binding activity (By similarity).Protease 3C: cleaves host DDX58/RIG-I and thus contributes to the inhibition of type I interferon production. Cleaves also host PABPC1 (By similarity).RNA-directed RNA polymerase: Replicates the viral genomic RNA on the surface of intracellular membranes. May form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated (By similarity). |
| Transmembrane Regions | |
| GenBank Protein ID | |
| UniProtKB ID | P21404 |
| UniProtKB Entry Name | |
| Cellular Location | Virion |
| Gene sequence | >lcl|BSEQ0008041|6606 bp ATGGGAGCTCAAGTGTCAACACAGAAAACTGGAGCTCATGAAACCAGTTTAAGTGCGGCA GGTAATTCAATTATACATTATACGAACATCAACTACTATAAAGATGCTGCGTCTAATTCG GCTAATCGGCAAGACTTTACACAAGACCCGAGTAAGTTTACAGAGCCTGTTAAAGATGTT ATGATTAAGTCTTTACCTGCCCTCAACTCACCAACAGTAGAAGAGTGCGGGTACAGCGAT CGGGTTAGGTCCATCACCCTTGGAAACTCCACGATAACCACGCAGGAGTGTGCTAACGTG GTGGTGGGGTATGGTAGATGGCCCACTTACCTCAGGGACGACGAGGCGACTGCCGAGGAT CAACCCACACAGCCTGACGTAGCAACATGCCGCTTTTATACTTTAGATTCAATCAAGTGG GAAAAGGGATCGGTGGGGTGGTGGTGGAAGTTCCCAGAAGCGCTTAGTGATATGGGATTA TTCGGTCAGAACATGCAATACCACTACCTGGGTCGTGCAGGGTACACTATTCACCTACAA TGTAACGCTTCCAAGTTCCATCAAGGGTGTTTGCTAGTAGTGTGTGTGCCCGAGGCTGAG ATGGGAGGAGCTGTGGTTGGACAAGCATTTTCCGCCACCGCGATGGCAAATGGTGATAAA GCATATGAGTTCACTAGCGCAACCCAAAGTGATCAGACAAAAGTTCAAACTGCTATACAC AATGCAGGGATGGGCGTAGGTGTAGGGAACCTCACTATCTACCCGCACCAGTGGATAAAT TTGCGCACCAACAACAGTGCCACCATAGTGATGCCATATATTAATAGTGTGCCCATGGAC AACATGTTCAGACATTATAATTTTACCCTGATGGTGATACCTTTTGTGAAACTGGACTAT GCCGACACCGCATCCACGTACGTGCCAATTACAGTGACGGTGGCCCCAATGTGTGCGGAG TATAACGGCTTACGTCTGGCACAAGCGCAAGGTTTGCCAACTATGAACACACCAGGAAGC ACGCAATTCCTAACATCAGATGACTTTCAATCGCCGTGCGCTTTGCCACAATTTGATGTG ACGCCTAGTATGAACATCCCAGGAGAAGTGAAGAACCTAATGGAAATAGCAGAAGTGGAC TCGGTCGTGCCCGTGAACAATGTCCAAGACACCACTGACCAAATGGAGATGTTCAGGATA CCAGTGACCATAAATGCCCCTCTACAACAACAGGTTTTTGGCCTCAGATTGCAACCAGGC TTAGATAGTGTGTTTAAGCACACTCTGTTGGGAGAAATTCTAAACTACTATGCGCACTGG TCAGGCAGCATGAAGCTGACATTTGTGTTTTGCGGGTCTGCAATGGCAACAGGGAAATTT TTAATAGCATATTCACCACCTGGGGCCAACCCCCCGAAAACACGAAAGGATGCAATGCTG GGAACACATATCATATGGGACATTGGTTTACAATCTAGCTGTGTGTTGTGTGTGCCATGG ATCAGTCAAACACATTATAGGCTTGTACAGCAGGATGAGTACACCAGCGCTGGTTACGTG ACGTGTTGGTATCAGACTGGTATGATTGTCCCACCAGGAACCCCAAATTCTAGCTCTATT ATGTGCTTTGCATCAGCGTGCAACGACTTCTCAGTAAGAATGTTGAGGGACACACCATTC ATATCCCAGGATAATAAGCTGCAAGGGGATGTGGAAGAAGCCATTGAGAGGGCACGTTGT ACAGTTGCTGACACCATGCGTACGGGGCCTAGCAATTCCGCGAGCGTACCTGCACTCACT GCAGTTGAGACAGGGCACACCTCGCAAGTTACTCCAAGTGACACTATGCAGACAAGACAT GTGAAAAACTATCATTCGCGCTCTGAGTCGACTGTGGAGAATTTCCTCGGTCGGTCGGCA TGCGTGTACATGGAAGAGTACAAGACCACTGATAAGCATGTTAACAAGAAATTCGTCGCC TGGCCAATCAACACAAAACAAATGGTTCAGATGCGGAGGAAGCTGGAAATGTTCACTTAT CTTAGGTTTGATATGGAGGTAACTTTTGTGATCACAAGTCGACAAGACCCCGGAACAACC CTAGCTCAGGACATGCCCGTGTTGACGCGCCAAATCATGTATGTGCCACCTGGCGGTCCG ATTCCAGCAAAAGTTGATGATTATGCCTGGCAGACGTCTACAAACCCCAGCATTTTCTGG ACGGAAGGAAACGCACCAGCGCGCATGTCCATCCCATTTATCAGCATAGGAAATGCATAC AGCAATTTTTATGACGGGTGGTCAAATTTTGATCAGAGGGGCTCATACGGGTACAATACC CTGAATAACTTAGGTCACATATATGTGAGACACGTGAGTGGAAGTAGTCCTCACCCAATC ACGAGCACTATTAGAGTGTATTTCAAGCCAAAACATACCAGAGCTTGGGTGCCGCGCCCT CCAAGGCTATGCCAATACAAGAAGGCATTTAGCGTGGATTTCACGCCAACTCCCATTACA GACACCAGGAAAGACATCAACACTGTAACCACCGTGGCGCAAAGTCGGCGTCGGGGTGAC ATGTCCACCCTTAACACGCATGGTGCCTTCGGACAACAATCCGGGGCCGTCTATGTGGGC AACTACAGAGTGATCAACAGACACCTGGCAACACACACGGATTGGCAAAATTGCGTGTGG GAGGATTACAATAGAGACCTCCTTGTGAGCACGACTACAGCGCACGGGTGTGATGTCATA GCTAGATGCCAGTGTACAACCGGGGTGTACTTTTGTGCATCCAAGAACAAGCACTACCCT GTTTCATTCGAAGGGCCAGGTTTAGTGGAAGTCCAAGAGAGTGAATACTACCCTAAAAGA TATCAATCCCATGTACTTCTGGCAGCAGGATTTTCCGAACCAGGGGACTGTGGTGGCATC TTAAGGTGTGAACACGGTGTCATAGGCATCGTAACCATGGGAGGCGAAGGTGTTGTGGGT TTTGCTGACGTGCGCGACCTCTTATGGTTAGAGGATGACGCTATGGAGCAGGGTGTGAAG GACTACGTGGAGCAACTTGGAAATGCATTTGGTTCAGGCTTCACCAATCAGATCTGTGAG CAAGTTAACCTTTTAAAAGAATCACTAGTGGGTCAAGACTCCATCTTAGAGAAATCTCTA AAAGCCCTAGTCAAGATAATATCAGCCTTAGTGATCGTGGTGAGGAACCACGATGACCTA ATTACAGTGACTGCCATACTAGCCCTCATCGGTTGCACCTCGTCTCCATGGCGGTGGCTT AAACAGAAAGTGTCACAGTATTATGGGATACCCATGGCTGAACGCCAAAATGATAGCTGG CTCAAGAAATTCACTGAAATGACAAATGCCTGCAAACGAATGGAGTGGATAGCCATCAAA ATTCAGAAGTTTATAGAGTGGCTCAAAGTTAAAATTCTACCAGAGGTAAGGGAGAAGCAT GAGTTCCTGAACAGACTTAAACAGCTTCCCCTATTAGAGAGTCAGATTGCCACCATCGAG CAGAGTGCACCATCCCAAAGCGACCAAGAGCAATTGTTTTCCAATGTCCAGTACTTTGCA CACTATTGCAGAAAGTATGCCCCCCTCTACGCAGCAGAGGCAAAGAGGGTGTTCTCCCTT GAGAAAAAGATGAGCAATTACATACAGTTCAAGTCCAAATGCCGTATTGAGCCTGTATGT TTGCTCCTACATGGGAGTCCAGGTGCCGGCAAGTCGGTGGCAACAAACCTAATTGGAAGG TCACTCGCTGAGAAATTAAACAGTTCAGTGTACTCATTACCACCAGACCCAGATCACTTT GATGGCTACAAACAACAGGCCGTAGTGATTATGGATGACCTATGCCAGAACCCTGATGGA AAAGATGTCTCCTTGTTTTGCCAAATGGTCTCTAGTGTAGATTTTGTGCCGCCCATGGCT GCCTTGGAAGAGAAGGGCATTTTGTTCACCTCTCCGTTCGTTCTGGCATCGACTAATGCA GGATCCATAAATGCTCCAACTGTGTCAGACAGCAGGGCCTTAGCAAGGAGGTTTCACTTC GATATGAACATTGAAGTTATCTCCATGTATAGTCAGAACGGCAAAATAAATATGCCGATG TCAGTGAAGACGTGTGATGAAGAGTGCTGTCCAGTCAACTTTAAGAAGTGTTGCCCTCTA GTATGTGGTAAAGCCATCCAGTTCATAGACAGAAGAACCCAAGTTAGATACTCCCTTGAC ATGCTGGTAACTGAGATGTTTAGAGAGTATAACCATAGGCATAGTGTCGGGGCCACCCTT GAGGCATTATTCCAGGGTCCACCGATATATAGAGAAATTAAGATCAGTGTTGCGCCAGAG ACACCACCACCACCTGTCATCGCTGATCTACTCAAGTCGGTGGACAGTGAGGATGTGAGA GAGTACTGCAAAGAAAAGGGATGGTTGATCCCTGAGGTAAACTCCACCCTCCAAATTGAA AAATACGTCAGTCGGGCTTTCATTTGCTTGCAGGCAATAACCACATTCGTGTCAGTAGCT GGGATCATCTACATAATATATAAGCTCTTTGCAGGCTTTCAGGGTGCATATACAGGAATA CCCAATCAGAAACCTAAGGTACCTACCTTAAGACAAGCAAAAGTGCAGGGTCCCGCATTT GAATTCGCCGTTGCAATGATGAAGAGAAACTCAAGCACGGTGAAGACTGAGTATGGCGAA TTCACCATGCTGGGCATCTATGACAGGTGGGCCGTCTTACCACGCCACGCTAAGCCTGGA CCAACCATCCTGATGAATGACCAGGAAGTGGGCGTGATGGATGCTAAGGAATTAGTGGAT AAGGATGGCACAAACCTAGAACTGACATTGCTTAAATTAAACAGGAATGAGAAGTTCAGA GACATCAGAGGCTTCTTAGCTAAGGAGGAGATGGAGGTCAACGAAGCCGTGCTAGCAATT AATACCAGTAAATTTCCCAACATGTACATTCCAGTGGGACAAGTCACGGACTACGGCTTC CTAAACCTGGGTGGTACACCCACTAAGAGAATGCTCATGTACAACTTCCCCACAAGAGCA GGTCAGTGCGGCGGAGTGCTCATGTCCACTGGCAAAGTCTTGGGAATCCATGTTGGTGGA AATGGTCATCAAGGTTTCTCAGCAGCACTTCTCAAGCACTACTTCAATGATGAACAAGGA GAGATCGAGTTCATTGAGAGTTCAAAGGATGCAGGGTTCCCGATTATCAATACACCTAGT AAGACCAAGCTGGAGCCAAGTGTCTTCCATCAAGTTTTTGAAGGTGTCAAAGAACCAGCG GTCCTCAGGAATGGTGATCCACGCCTCAAAGCTAATTTTGAGGAAGCCATATTTTCCAAG TACATCGGAAATGTTAACACGCACGTGGATGAATACATGCTGGAAGCTGTTGATCATTAT GCCGGACAATTGGCCACCCTAGATATTAGCACTGAACCAATGAAGTTGGAGGATGCTGTA TACGGTACTGAAGGTCTTGAGGCTCTTGACCTAACAACGAGTGCAGGTTACCCTTATGTT GCTCTGGGCATCAAGAAGAGGGACATTCTCTCAAAGAAGACCAGGGACCTGACCAAGCTG AAGGAGTGCATGGACAAGTATGGCCTAAACTTGCCAATGATAACCTATGTGAAAGATCAA CTCAGATCTGCAGAGAAGGTGGCAAAGGGAAAGTCTAGGCTCATTGAGGCGTCCAGTTTG AATGACTCCGTGGCAATGAGACAGACATTCGGCAACCTATACAAAACTTTTCACCTAAAC CCAGGGATTGTGACTGGCAGTGCCGTCGGGTGTGACCCGGATCTCTTTTGGAGTAAAATA CCAGTAATGTTAAACGGTCACCTCATAGCCTTTGATTACTCTGGATATGATGCTAGCTTG AGTCCTGTATGGTTTGCTTGTCTAAAACTACTACTTGAGAAACTTGGTTACTCGCACAAG GAGACCAATTACATTGATTACCTGTGCAACTCCCATCACCTGTACAGGGACAAGCATTAT TTCGTGCGGGGTGGCATGCCATCAGGATGTTCTGGCACGAGCATCTTTAACTCAATGATA AACAACATCATAATTAGGACACTCATGTTGAAGGTTTACAAAGGGATCGACTTGGATCAA TTCAGGATGATTGCTTATGGTGACGATGTGATCGCATCATACCCGTGGCCCATAGATGCG TCTTTGCTTGCTGAAGCTGGCAAGGACTATGGATTAATCATGACACCAGCAGACAAAGGG GAGTGCTTCAATGAAGTTACTTGGACTAACGTCACATTCCTAAAGAGGTATTTTAGAGCA GATGAACAATACCCCTTTTTAGTGCACCCTGTTATGCCTATGAAAGACATACACGAATCA ATCAGATGGACCAAGGATCCAAAGAACACCCAAGACCACGTGCGCTCGCTATGCTTATTA GCTTGGCACAACGGGGAGCACGAATATGAGGAGTTCATTCGCAAAATCAGGAGCGTCCCA GTTGGACGTTGTTTGACCCTACCTGCGTTCTCAACCCTACGCAGGAAGTGGTTGGACTCT TTCTAA |
| GenBank Gene ID | |
| GeneCard ID | None |
| GenAtlas ID | |
| HGNC ID | |
| Chromosome Location | None |
| Locus | None |
| References |
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From www.t3db.ca