Genome polyprotein
| Name | Genome polyprotein |
|---|---|
| Synonyms |
|
| Gene Name | None |
| Organism | Poliovirus type 2 (strain Lansing) |
| Amino acid sequence | >lcl|BSEQ0012951|Genome polyprotein MGAQVSSQKVGAHENSNRAYGGSTINYTTINYYRDSASNAASKQDFAQDPSKFTEPIKDV LIKTAPTLNSPNIEACGYSDRVMQLTLGNSTITTQEAANSVVAYGRWPEYIKDSEANPVD QPTEPDVAACRFYTLDTVTWRKESRGWWWKLPDALKDMGLFGQNMFYHYLGRAGYTVHVQ CNASKFHQGALGVFAVPEMCLAGDSTTHMFTKYENANPGEKGGEFKGSFTLDTNATNPAR NFCPVDYLFGSGVLAGNAFVYPHQIINLRTNNCATLVLPYVNSLSIDSMTKHNNWGIAIL PLAPLDFATESSTEIPITLTIAPMCCEFNGLRNITVPRTQGLPVLNTPGSNQYLTADNYQ SPCAIPEFDVTPPIDIPGEVRNMMELAEIDTMIPLNLTNQRKNTMDMYRVELNDAAHSDT PILCLSLSPASDPRLAHTMLGEILNYYTHWAGSLKFTFLFCGSMMATGKLLVSYAPPGAE APKSRKEAMLGTHVIWDIGLQSSCTMVVPWISNTTYRQTINDSFTEGGYISMFYQTRVVV PLSTPRKMDILGFVSACNDFSVRLLRDTTHISQEAMPQGLGDLIEGVVEGVTRNALTPLT PANNLPDTQSSGPAHSKETPALTAVETGATNPLVPSDTVQTRHVIQKRTRSESTVESFFA RGACVAIIEVDNDAPTKRASKLFSVWKITYKDTVQLRRKLEFFTYSRFDMEFTFVVTSNY TDANNGHALNQVYQIMYIPPGAPIPGKWNDYTWQTSSNPSVFYTYGAPPARISVPYVGIA NAYSHFYDGFAKVPLAGQASTEGDSLYGAASLNDFGSLAVRVVNDHNPTKLTSKIRVYMK PKHVRVWCPRPPRAVPYYGPGVDYKDGLAPLPGKGLTTYGFGHQNKAVYTAGYKICNYHL ATQEDLQNAVNIMWIRDLLVVESKAQGIDSIARCNCHTGVYYCESRRKYYPVSFTGPTFQ YMEANEYYPARYQSHMLIGHGFASPGDCGGILRCQHGVIGIITAGGEGLVAFSDIRDLYA YEEEAMEQGVSNYIESLGAAFGSGFTQQIGNKISELTSMVTSTITEKLLKNLIKIISSLV IITRNYEDTTTVLATLALLGCDASPWQWLKKKACDILEIPYIMRQGDSWLKKFTEACNAA KGLEWVSNKISKFIDWLKEKIIPQARDKLEFVTKLKQLEMLENQIATIHQSCPSQEHQEI LFNNVRWLSIQSKRFAPLYAVEAKRIQKLEHTINNYVQFKSKHRIEPVCLLVHGSPGTGK SVATNLIARAIAEKENTSTYSLPPDPSHFDGYKQQGVVIMDDLNQNPDGADMKLFCQMVS TVEFIPPMASLEEKGILFTSNYVLASTNSSRITPPTVAHSDALARRFAFDMDIQIMSEYS RDGKLNMAMATEMCKNCHHPANFKRCCPLVCGKAIQLMDKSSRVRYSIDQITTMIINERN RRSSIGNCMEALFQGPLQYKDLKIDIKTTPPPECINDLLQAVDSQEVRDYCEKKGWIVDI TSQVQTERNINRAMTILQAVTTFAAVAGVVYVMYKLFAGHQGAYTGLPNKRPNVPTIRTA KVQGPGFDYAVAMAKRNILTATTIKGEFTMLGVHDNVAILPTHASPGETIVIDGKEVEVL DAKALEDQAGTNLEITIVTLKRNEKFRDIRPHIPTQITETNDGVLIVNTSKYPNMYVPVG AVTEQGYLNLSGRQTARTLMYNFPTRAGQCGGVITCTGKVIGMHVGGNGSHGFAAALKRS YFTQSQGEIQWMRPSKEVGYPVINAPSKTKLEPSAFHYVFEGVKEPAVLTKSDPRLKTDF EEAIFSKYVGNKITEVDEYMKEAVDHYAGQLMSLDINTEQMCLEDAMYGTDGLEALDLST SAGYPYVAMGKKKRDILNKQTRDTKEMQRLLDTYGINLPLVTYVKDELRSKTKVEQGKSR LIEASSLNDSVAMRMAFGNLYAAFHKNPGVVTGSAVGCDPDLFWSKIPVLMEEKLFAFDY TGYDASLSPAWFEALKMVLEKIGFGDRVDYIDYLNHSHHLYKNKTYCVKGGMPSGCSGTS IFNSMINNLIIRTLLLKTYKGIDLDHLKMIAYGDDVIASYPHEVDASLLAQSGKDYGLTM TPADKSATFETVTWENVTFLKRFFRADEKYPFLVHPVMPMKEIHESIRWTKDPRNTQDHV RSLCLLAWHNGEEEYNKFLAKIRSVPIGRALLLPEYSTLYRRWLDSF |
| Number of residues | None |
| Molecular Weight | 245828.56 |
| Theoretical pI | 7.17 |
| GO Classification |
Functions
Processes
Components
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| General Function | Structural molecule activity |
| Specific Function | Capsid protein VP1: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome. Capsid protein VP1 mainly forms the vertices of the capsid. Capsid protein VP1 interacts with host cell receptor to provide virion attachment to target host cells. This attachment induces virion internalization through clathrin- and caveolin-independent endocytosis in Hela cells and through caveolin-mediated endocytosis in brain microvascular endothelial cells. Tyrosine kinases are probably involved in the entry process. After binding to its receptor, the capsid undergoes conformational changes. Capsid protein VP1 N-terminus (that contains an amphipathic alpha-helix) and capsid protein VP4 are externalized. Together, they shape a pore in the host membrane through which viral genome is translocated to host cell cytoplasm. After genome has been released, the channel shrinks (By similarity).Capsid protein VP2: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity).Capsid protein VP3: Forms an icosahedral capsid of pseudo T=3 symmetry with capsid proteins VP2 and VP3. The capsid is 300 Angstroms in diameter, composed of 60 copies of each capsid protein and enclosing the viral positive strand RNA genome (By similarity).Capsid protein VP4: Lies on the inner surface of the capsid shell. After binding to the host receptor, the capsid undergoes conformational changes. Capsid protein VP4 is released, Capsid protein VP1 N-terminus is externalized, and together, they shape a pore in the host membrane through which the viral genome is translocated into the host cell cytoplasm. After genome has been released, the channel shrinks (By similarity).Capsid protein VP0: Component of immature procapsids, which is cleaved into capsid proteins VP4 and VP2 after maturation. Allows the capsid to remain inactive before the maturation step (By similarity).Protein 2A: Cysteine protease that cleaves viral polyprotein and specific host proteins. It is responsible for the cleavage between the P1 and P2 regions, first cleavage occurring in the polyprotein. Cleaves also the host translation initiation factor EIF4G1, in order to shut down the capped cellular mRNA translation. Inhibits the host nucleus-cytoplasm protein and RNA trafficking by cleaving host members of the nuclear pores (By similarity).Protein 2B: Plays an essential role in the virus replication cycle by acting as a viroporin. Creates a pore in the host reticulum endoplasmic and as a consequence releases Ca2+ in the cytoplasm of infected cell. In turn, high levels of cyctoplasmic calcium may trigger membrane trafficking and transport of viral ER-associated proteins to viroplasms, sites of viral genome replication (By similarity).Protein 2C: Induces and associates with structural rearrangements of intracellular membranes. Displays RNA-binding, nucleotide binding and NTPase activities. May play a role in virion morphogenesis and viral RNA encapsidation by interacting with the capsid protein VP3 (By similarity).Protein 3AB: Localizes the viral replication complex to the surface of membranous vesicles. Together with protein 3CD binds the Cis-Active RNA Element (CRE) which is involved in RNA synthesis initiation. Acts as a cofactor to stimulate the activity of 3D polymerase, maybe through a nucleid acid chaperone activity (By similarity).Protein 3A: Localizes the viral replication complex to the surface of membranous vesicles. It inhibits host cell endoplasmic reticulum-to-Golgi apparatus transport and causes the dissassembly of the Golgi complex, possibly through GBF1 interaction. This would result in depletion of MHC, trail receptors and IFN receptors at the host cell surface (By similarity).Viral protein genome-linked: acts as a primer for viral RNA replication and remains covalently bound to viral genomic RNA. VPg is uridylylated prior to priming replication into VPg-pUpU. The oriI viral genomic sequence may act as a template for this. The VPg-pUpU is then used as primer on the genomic RNA poly(A) by the RNA-dependent RNA polymerase to replicate the viral genome. VPg may be removed in the cytoplasm by an unknown enzyme termed "unlinkase". VPg is not cleaved off virion genomes because replicated genomic RNA are encapsidated at the site of replication (By similarity).Protein 3CD: Is involved in the viral replication complex and viral polypeptide maturation. It exhibits protease activity with a specificity and catalytic efficiency that is different from protease 3C. Protein 3CD lacks polymerase activity. The 3C domain in the context of protein 3CD may have an RNA binding activity (By similarity).Protease 3C: cleaves host DDX58/RIG-I and thus contributes to the inhibition of type I interferon production. Cleaves also host PABPC1 (By similarity).RNA-directed RNA polymerase: Replicates the viral genomic RNA on the surface of intracellular membranes. May form linear arrays of subunits that propagate along a strong head-to-tail interaction called interface-I. Covalently attaches UMP to a tyrosine of VPg, which is used to prime RNA synthesis. The positive stranded RNA genome is first replicated at virus induced membranous vesicles, creating a dsRNA genomic replication form. This dsRNA is then used as template to synthesize positive stranded RNA genomes. ss(+)RNA genomes are either translated, replicated or encapsidated (By similarity). |
| Transmembrane Regions | |
| GenBank Protein ID | |
| UniProtKB ID | P06210 |
| UniProtKB Entry Name | |
| Cellular Location | Virion |
| Gene sequence | >lcl|BSEQ0008043|6624 bp ATGGGCGCCCAAGTCTCATCACAGAAAGTTGGAGCCCATGAGAATTCAAACAGAGCTTAT GGCGGATCCACCATTAATTACACTACTATTAATTATTACAGGGATTCTGCGAGCAATGCC GCTAGTAAGCAGGACTTTGCACAAGACCCATCCAAGTTCACTGAACCTATTAAAGATGTT CTCATTAAGACCGCTCCCACACTAAACTCTCCTAATATCGAGGCGTGTGGGTATAGCGAC AGAGTGATGCAACTAACCCTAGGCAATTCCACCATTACCACACAGGAGGCGGCCAATTCT GTCGTTGCATACGGCCGGTGGCCCGAGTACATCAAGGACTCAGAAGCAAATCCTGTGGAC CAGCCAACTGAACCGGACGTTGCCGCGTGCAGGTTTTACACACTAGACACTGTTACTTGG CGCAAGGAGTCCAGAGGGTGGTGGTGGAAACTGCCTGATGCACTAAAGGACATGGGATTA TTCGGCCAGAACATGTTCTACCACTACCTCGGGAGGGCTGGCTATACTGTGCACGTACAG TGTAATGCTTCAAAGTTTCACCAGGGCGCCCTCGGGGTATTCGCAGTTCCAGAAATGTGC CTGGCAGGCGACAGCACAACCCACATGTTTACAAAATATGAGAATGCAAATCCGGGTGAG AAAGGGGGTGAATTCAAAGGGAGTTTTACTCTGGATACTAACGCTACCAACCCTGCACGC AACTTTTGTCCCGTTGATTATCTCTTCGGGAGCGGAGTACTGGCGGGAAATGCGTTTGTT TACCCACATCAGATAATTAATCTGCGCACCAACAACTGTGCCACGTTGGTGCTGCCATAC GTTAATTCACTTTCCATAGACAGCATGACAAAACACAACAATTGGGGAATTGCTATCCTT CCGCTGGCACCACTTGACTTTGCCACCGAGTCCTCCACTGAGATACCCATTACTCTAACT ATTGCCCCTATGTGTTGTGAATTCAATGGGTTGCGCAACATCACTGTACCCAGAACTCAA GGGTTGCCAGTCTTAAACACTCCAGGAAGCAACCAGTACTTAACAGCAGACAACTATCAA TCCCCATGTGCGATACCCGAGTTTGATGTAACACCACCCATAGACATCCCGGGGGAAGTG CGCAACATGATGGAATTGGCAGAGATAGACACCATGATACCTCTCAATCTGACGAACCAG CGCAAGAACACCATGGATATGTACAGAGTCGAACTGAATGATGCGGCTCACTCTGACACA CCAATATTGTGTCTCTCACTGTCTCCAGCATCAGATCCTAGGCTAGCACACACTATGCTA GGTGAAATACTGAACTACTACACACACTGGGCAGGGTCATTGAAGTTCACATTTCTCTTC TGCGGCTCAATGATGGCCACTGGTAAATTGCTAGTGTCCTATGCACCTCCTGGTGCGGAA GCCCCTAAAAGCCGCAAAGAAGCGATGCTCGGCACCCACGTGATCTGGGACATCGGATTA CAGTCATCATGCACTATGGTGGTACCTTGGATTAGCAACACCACATACAGACAAACCATC AACGATAGCTTCACAGAAGGAGGGTACATCAGTATGTTTTACCAAACTAGAGTTGTTGTG CCATTGTCCACCCCTAGAAAGATGGACATATTGGGCTTTGTGTCAGCCTGCAATGACTTC AGTGTGCGCCTGTTGCGTGACACGACGCACATAAGCCAAGAGGCTATGCCACAAGGATTG GGTGATTTAATTGAAGGGGTTGTTGAGGGAGTCACGAGAAATGCCTTGACACCACTGACA CCTGCCAACAACTTGCCTGATACACAATCTAGCGGCCCAGCCCACTCTAAGGAAACACCA GCGCTAACAGCCGTAGAGACAGGGGCCACCAACCCATTGGTGCCTTCAGACACGGTACAA ACTCGTCACGTCATCCAAAAGCGGACGCGGTCGGAGTCTACGGTTGAGTCTTTCTTCGCA AGAGGAGCTTGTGTGGCCATTATTGAAGTGGATAATGATGCTCCAACAAAGCGTGCCAGT AAATTATTTTCAGTCTGGAAGATAACTTACAAAGACACCGTTCAGTTAAGACGTAAGTTG GAGTTCTTTACATATTCAAGGTTTGACATGGAGTTCACCTTTGTGGTTACATCCAATTAT ACCGATGCAAACAATGGGCACGCACTAAATCAAGTTTACCAGATAATGTACATACCACCT GGGGCACCGATCCCTGGCAAGTGGAATGATTACACATGGCAAACGTCATCTAACCCATCA GTGTTTTACACTTACGGGGCACCTCCAGCTAGAATATCAGTGCCCTACGTGGGCATTGCC AATGCATATTCTCATTTTTACGATGGGTTTGCCAAAGTACCACTAGCAGGCCAAGCCTCA ACAGAGGGTGACTCGCTGTATGGAGCGGCTTCATTGAATGACTTCGGATCACTGGCTGTT CGAGTGGTGAATGACCACAACCCTACGAAACTCACCTCAAAAATCAGAGTGTACATGAAA CCAAAGCACGTCAGAGTGTGGTGTCCGCGACCCCCTCGAGCAGTCCCATACTACGGACCA GGGGTTGACTACAAGGATGGACTAGCCCCACTGCCAGGGAAAGGCTTGACAACCTATGGT TTTGGCCACCAAAATAAGGCAGTGTACACGGCAGGTTACAAAATTTGCAATTACCACCTC GCCACCCAGGAAGACTTACAAAATGCGGTAAACATTATGTGGATTAGAGACCTTTTAGTA GTGGAATCCAAAGCCCAAGGCATAGACTCAATTGCTAGATGTAACTGCCACACTGGAGTG TACTACTGTGAATCCAGGAGGAAGTACTACCCGGTCTCTTTTACTGGCCCCACCTTTCAG TACATGGAAGCAAATGAGTACTATCCAGCCCGATACCAATCCCACATGTTAATTGGCCAT GGTTTTGCATCTCCAGGGGACTGTGGTGGGATTCTCAGGTGCCAACATGGAGTAATTGGA ATCATTACAGCTGGAGGAGAAGGCCTAGTCGCTTTCTCGGACATCAGAGATCTGTACGCA TACGAGGAGGAGGCTATGGAGCAGGGAGTCTCCAACTATATTGAGTCCCTTGGGGCTGCA TTTGGGAGTGGATTCACCCAGCAAATAGGAAACAAAATTTCAGAACTCACTAGCATGGTC ACCAGCACTATAACTGAGAAACTACTAAAGAATCTCATTAAAATAATTTCATCCCTTGTT ATCATCACCAGAAACTATGAAGACACGACCACAGTGCTGGCTACCCTTGCTCTCCTCGGT TGTGATGCGTCCCCATGGCAATGGCTAAAGAAGAAAGCCTGTGACATCTTGGAAATCCCC TACATCATGCGACAGGGCGATAGCTGGTTGAAGAAGTTTACAGAGGCATGCAATGCAGCT AAGGGATTGGAATGGGTGTCTAATAAAATATCCAAATTTATTGACTGGCTCAAAGAGAAG ATCATTCCACAGGCTAGAGACAAGCTAGAGTTTGTTACCAAACTGAAGCAACTAGAAATG TTGGAGAACCAAATTGCAACCATTCATCAATCGTGCCCAAGTCAGGAGCATCAAGAAATC CTGTTCAATAACGTGAGATGGTTATCCATACAGTCAAAGAGATTTGCCCCGCTCTATGCG GTTGAGGCTAAGAGAATACAAAAGTTAGAGCACACGATTAACAACTACGTACAGTTCAAG AGCAAACACCGTATTGAACCAGTATGTTTGTTGGTGCACGGTAGCCCAGGCACGGGCAAG TCAGTTGCCACCAATTTAATTGCCAGAGCAATAGCAGAGAAGGAGAACACCTCCACATAC TCACTACCACCAGATCCCTCCCATTTCGATGGGTACAAGCAACAAGGTGTGGTGATCATG GATGATTTGAATCAGAACCCAGACGGAGCAGACATGAAGCTGTTTTGTCAGATGGTCTCC ACTGTAGAATTCATACCACCAATGGCTTCGCTAGAAGAAAAGGGTATTTTGTTCACATCT AATTACGTTTTGGCCTCAACCAATTCCAGTCGCATCACCCCACCAACTGTTGCGCACAGC GATGCCCTAGCCAGGCGCTTTGCATTTGACATGGACATACAAATCATGAGCGAGTATTCT AGAGATGGAAAATTGAACATGGCGATGGCAACTGAAATGTGTAAGAACTGTCATCACCCA GCAAACTTCAAGAGATGTTGCCCATTGGTGTGTGGCAAAGCCATCCAGCTGATGGACAAA TCTTCCAGAGTCAGATATAGTATAGATCAGATTACTACCATGATTATTAATGAGAGGAAC AGAAGATCAAGTATCGGTAATTGCATGGAGGCACTTTTCCAAGGTCCTCTTCAATACAAA GACCTGAAAATAGACATTAAGACCACACCTCCTCCTGAGTGCATCAATGATTTGCTCCAA GCAGTTGATTCTCAAGAGGTAAGAGACTACTGTGAGAAGAAGGGTTGGATAGTAGACATC ACTAGTCAGGTGCAAACCGAAAGAAACATCAATAGAGCAATGACTATTCTTCAGGCGGTC ACCACATTTGCCGCAGTTGCTGGAGTGGTGTATGTGATGTACAAACTCTTTGCAGGGCAT CAAGGAGCGTATACAGGGCTTCCCAATAAGAGACCCAATGTCCCCACCATCAGGACTGCC AAGGTTCAGGGCCCAGGATTTGACTACGCAGTGGCAATGGCCAAAAGAAACATTCTTACG GCAACTACCATTAAGGGAGAGTTCACAATGCTCGGAGTGCATGATAATGTGGCCATTCTA CCAACCCACGCATCACCGGGTGAAACAATAGTCATTGATGGCAAGGAAGTAGAGGTACTG GATGCTAAAGCCCTGGAGGACCAGGCCGGGACCAACCTGGAAATCACCATTGTCACTCTT AAGAGAAATGAGAAGTTCAGGGACATCAGACCACACATCCCCACTCAAATCACTGAGACA AATGATGGAGTTTTAATTGTGAACACTAGTAAGTACCCCAACATGTATGTTCCTGTCGGT GCTGTGACTGAACAGGGGTATCTCAATCTCAGTGGACGCCAAACTGCTCGTACTTTAATG TACAACTTTCCAACGAGAGCAGGTCAATGTGGTGGAGTTATCACCTGCACTGGCAAGGTC ATCGGGATGCATGTTGGTGGGAACGGTTCACATGGGTTCGCAGCAGCCCTGAAGCGATCC TATTTCACTCAGAGTCAAGGTGAAATCCAGTGGATGAGACCATCAAAAGAAGTGGGCTAC CCCGTTATTAATGCTCCATCTAAAACTAAACTGGAACCCAGTGCATTCCATTATGTGTTT GAAGGTGTCAAGGAACCAGCTGTGCTCACCAAAAGTGACCCCAGATTGAAGACAGATTTT GAAGAGGCTATCTTTTCCAAGTATGTGGGAAATAAGATTACTGAAGTGGATGAGTACATG AAAGAAGCTGTCGATCATTACGCAGGCCAGCTCATGTCACTAGACATCAACACAGAACAA ATGTGCCTTGAGGATGCAATGTATGGCACTGACGGTCTCGAAGCTCTAGACCTCAGTACC AGTGCTGGGTATCCCTATGTGGCAATGGGGAAAAAGAAAAGAGACATTTTGAATAAGCAA ACCAGAGACACAAAGGAAATGCAAAGGCTTCTGGACACCTATGGTATTAATTTACCTTTA GTCACCTATGTGAAAGATGAGCTTAGATCCAAGACCAAAGTGGAACAGGGCAAGTCCAGG CTAATTGAGGCCTCAAGTCTCAATGACTCTGTCGCCATGAGGATGGCTTTTGGCAACTTG TACGCAGCATTCCACAAGAACCCAGGTGTAGTGACAGGATCGGCTGTTGGCTGTGACCCA GATTTGTTTTGGAGTAAAATACCAGTCCTCATGGAGGAAAAACTCTTTGCATTTGATTAC ACGGGTTATGATGCTTCACTAAGCCCCGCCTGGTTTGAGGCTCTCAAGATGGTTCTAGAG AAAATTGGGTTTGGTGACAGAGTGGATTACATTGATTATCTGAATCACTCGCACCATCTA TATAAAAATAAGACATATTGTGTTAAGGGCGGCATGCCATCTGGCTGCTCTGGCACCTCA ATTTTTAATTCAATGATTAATAATCTAATAATCAGGACTCTCTTACTGAAAACCTACAAG GGCATAGATTTAGACCACCTGAAGATGATAGCCTATGGTGATGATGTAATTGCTTCCTAC CCCCATGAGGTTGATGCTAGTCTCCTAGCCCAATCAGGAAAAGACTATGGACTAACCATG ACACCAGCTGACAAATCAGCCACCTTTGAAACAGTCACATGGGAGAATGTAACATTCTTG AAAAGATTCTTTAGAGCAGATGAAAAGTATCCCTTTCTGGTACATCCAGTGATGCCAATG AAAGAAATTCACGAATCAATTAGATGGACTAAAGATCCCAGAAACACTCAGGATCATGTT CGCTCACTGTGCTTATTGGCTTGGCACAATGGCGAGGAAGAGTACAATAAATTTTTAGCT AAGATTAGAAGTGTGCCAATCGGAAGAGCATTACTGCTCCCTGAGTACTCCACATTGTAC CGCCGTTGGCTCGACTCATTTTAG |
| GenBank Gene ID | |
| GeneCard ID | None |
| GenAtlas ID | |
| HGNC ID | |
| Chromosome Location | None |
| Locus | None |
| References |
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From www.t3db.ca