Ammonia channel

NameAmmonia channel
Synonyms
  • Ammonia transporter
  • ybaG
Gene NameamtB
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0020523|Ammonia channel
MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGLIRG
KNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINWLMLKNIELTAVMGSIYQY
IHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTLSYIPIAHMVWGGGLLASHGA
LDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPHNLPMVFTGTAILYIGWFGFNAG
SAGTANEIAALAFVNTVVATAAAILGWIFGEWALRGKPSLLGACSGAIAGLVGVTPACGY
IGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPCDVFGVHGVCGIVGCIMTGIFAASSLG
GVGFAEGVTMGHQLLVQLESIAITIVWSGVVAFIGYKLADLTVGLRVPEEQEREGLDVNS
HGENAYNA
Number of residuesNone
Molecular Weight44514.335
Theoretical pI7.17
GO Classification
Functions
  • ammonium transmembrane transporter activity
  • uniporter activity
Processes
  • carbon dioxide transport
  • ammonium transport
Components
  • plasma membrane
  • integral component of plasma membrane
General FunctionUniporter activity
Specific FunctionInvolved in the uptake of ammonia.
Transmembrane Regions29-55 60-90 119-141 147-170 185-203 216-242 246-275 280-294 303-328 333-354 370-402
GenBank Protein ID
UniProtKB IDP69681
UniProtKB Entry Name
Cellular LocationCell inner membrane
Gene sequence
>lcl|BSEQ0020524|Ammonia channel (amtB)
ATGAAGATAGCGACGATAAAAACTGGGCTTGCTTCACTGGCGATGCTTCCGGGACTGGTA
ATGGCTGCACCTGCGGTGGCCGATAAAGCCGACAATGCGTTTATGATGATTTGTACTGCG
CTGGTGCTGTTTATGACTATTCCGGGGATTGCCCTGTTTTACGGTGGGTTGATTCGCGGC
AAAAACGTGCTGTCGATGCTGACGCAGGTGACGGTGACATTTGCACTGGTCTGTATTCTC
TGGGTGGTTTACGGTTACTCGCTGGCGTTTGGTGAGGGCAACAACTTCTTCGGCAACATT
AACTGGTTGATGCTGAAAAACATCGAACTGACGGCGGTGATGGGCAGCATTTATCAGTAT
ATCCACGTGGCGTTTCAGGGATCGTTTGCCTGCATTACCGTCGGCTTGATAGTTGGGGCG
CTGGCGGAACGAATCCGCTTCTCAGCTGTGTTGATTTTCGTGGTGGTATGGCTGACGCTC
TCTTACATTCCGATTGCGCATATGGTGTGGGGCGGTGGTTTGCTGGCTTCTCACGGTGCG
CTGGATTTCGCGGGTGGCACCGTGGTGCACATTAACGCCGCAATCGCCGGTCTGGTGGGC
GCGTATCTGATAGGAAAACGCGTGGGCTTCGGTAAAGAGGCGTTTAAACCGCACAACCTG
CCGATGGTCTTCACCGGGACTGCCATTCTCTATATCGGTTGGTTTGGCTTTAACGCCGGG
TCAGCGGGCACGGCGAATGAAATCGCGGCACTGGCATTTGTGAATACTGTGGTCGCAACG
GCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCACTG
CTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTAC
ATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGC
GTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCAC
GGCGTTTGTGGCATTGTCGGCTGTATCATGACCGGGATTTTTGCCGCCAGCTCGCTGGGC
GGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGC
ATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGAT
CTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGC
CACGGCGAGAATGCCTATAACGCGTAA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. van Heeswijk WC, Hoving S, Molenaar D, Stegeman B, Kahn D, Westerhoff HV: An alternative PII protein in the regulation of glutamine synthetase in Escherichia coli. Mol Microbiol. 1996 Jul;21(1):133-46. [8843440 ]
  2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [9278503 ]
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [16738553 ]
  4. Naggert J, Narasimhan ML, DeVeaux L, Cho H, Randhawa ZI, Cronan JE Jr, Green BN, Smith S: Cloning, sequencing, and characterization of Escherichia coli thioesterase II. J Biol Chem. 1991 Jun 15;266(17):11044-50. [1645722 ]
  5. Borodovsky M, Rudd KE, Koonin EV: Intrinsic and extrinsic approaches for detecting genes in a bacterial genome. Nucleic Acids Res. 1994 Nov 11;22(22):4756-67. [7984428 ]
  6. Thomas GH, Mullins JG, Merrick M: Membrane topology of the Mep/Amt family of ammonium transporters. Mol Microbiol. 2000 Jul;37(2):331-44. [10931328 ]
  7. Thomas G, Coutts G, Merrick M: The glnKamtB operon. A conserved gene pair in prokaryotes. Trends Genet. 2000 Jan;16(1):11-4. [10637624 ]
  8. Blakey D, Leech A, Thomas GH, Coutts G, Findlay K, Merrick M: Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry. Biochem J. 2002 Jun 1;364(Pt 2):527-35. [12023896 ]
  9. Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G: Global topology analysis of the Escherichia coli inner membrane proteome. Science. 2005 May 27;308(5726):1321-3. [15919996 ]
  10. Zheng L, Kostrewa D, Berneche S, Winkler FK, Li XD: The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17090-5. Epub 2004 Nov 24. [15563598 ]
  11. Khademi S, O'Connell J 3rd, Remis J, Robles-Colmenares Y, Miercke LJ, Stroud RM: Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A. Science. 2004 Sep 10;305(5690):1587-94. [15361618 ]

From www.t3db.ca