Putrescine-binding periplasmic protein

NamePutrescine-binding periplasmic protein
Synonyms
  • Putrescine-binding periplasmic protein precursor
Gene NamepotF
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0010926|Putrescine-binding periplasmic protein
MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPDTVANFEKETGIKVVYD
VFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLDKSKLPEWKNLDPELLKLV
AKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSWDLILKPENLEKLKSCGVSFL
DAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLRPNIRYFHSSQYINDLANGDICV
AIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAFFDVFAMPADAKNKDEAYQFLNYLL
RPDVVAHISDHVFYANANKAATPLVSAEVRENPGIYPPADVRAKLFTLKVQDPKIDRVRT
RAWTKVKSGK
Number of residuesNone
Molecular Weight40839.305
Theoretical pI6.08
GO Classification
Functions
  • polyamine binding
  • putrescine-importing ATPase activity
Processes
  • putrescine transport
Components
  • outer membrane-bounded periplasmic space
General FunctionPutrescine-importing atpase activity
Specific FunctionRequired for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP31133
UniProtKB Entry Name
Cellular LocationPeriplasm
Gene sequence
>lcl|BSEQ0010927|Putrescine-binding periplasmic protein (potF)
ATGACCGCCTTAAATAAAAAATGGCTATCGGGTCTGGTTGCGGGTGCTCTGATGGCCGTC
TCTGTCGGCACGCTCGCGGCTGAACAAAAAACACTCCACATTTATAACTGGTCTGATTAT
ATCGCCCCGGACACGGTGGCCAATTTTGAAAAAGAAACCGGTATTAAAGTCGTCTACGAT
GTTTTCGACTCTAACGAAGTACTGGAAGGCAAATTAATGGCCGGGAGTACCGGCTTTGAT
CTGGTGGTTCCATCTGCCAGCTTTCTGGAGCGCCAGTTGACTGCGGGAGTTTTCCAGCCG
CTGGACAAAAGCAAATTGCCGGAGTGGAAGAATCTCGACCCGGAACTGCTGAAGCTGGTC
GCCAAACACGATCCCGACAATAAATTTGCTATGCCCTATATGTGGGCGACGACCGGGATT
GGCTATAACGTTGATAAAGTTAAAGCGGTGCTGGGCGAAAACGCGCCCGTCGATAGCTGG
GACTTGATCCTCAAACCTGAAAATCTGGAAAAACTGAAAAGCTGCGGTGTCTCTTTCCTG
GATGCGCCAGAAGAAGTTTTTGCTACCGTGTTGAATTATCTCGGCAAAGATCCCAACAGC
ACTAAAGCGGATGATTACACCGGACCGGCAACAGATCTGCTGTTAAAGCTGCGCCCGAAC
ATTCGTTATTTCCATTCATCTCAATACATTAACGACCTGGCAAACGGCGATATTTGCGTC
GCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAAT
GGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGTTCTTTGATGTATTC
GCCATGCCTGCGGATGCCAAAAACAAAGACGAAGCCTATCAGTTCCTGAATTACCTGCTG
CGCCCGGATGTAGTAGCGCATATTTCCGACCATGTGTTCTATGCCAACGCCAATAAAGCA
GCCACGCCGCTGGTGAGTGCGGAAGTCCGTGAGAACCCAGGTATTTATCCGCCTGCGGAT
GTTCGTGCGAAGCTGTTCACTCTGAAAGTGCAGGATCCGAAAATCGACCGTGTGCGCACC
CGCGCGTGGACCAAAGTGAAGAGCGGAAAATAA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Pistocchi R, Kashiwagi K, Miyamoto S, Nukui E, Sadakata Y, Kobayashi H, Igarashi K: Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome. J Biol Chem. 1993 Jan 5;268(1):146-52. [8416922 ]
  2. Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [8905232 ]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [9278503 ]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [16738553 ]
  5. Link AJ, Robison K, Church GM: Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis. 1997 Aug;18(8):1259-313. [9298646 ]
  6. Vassylyev DG, Kashiwagi T, Tomitori H, Kashiwagi K, Igarashi K, Morikawa K: Crystallization and preliminary X-ray analysis of the periplasmic receptor (PotF) of the putrescine transport system in Escherichia coli. Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):132-4. [9761835 ]
  7. Vassylyev DG, Tomitori H, Kashiwagi K, Morikawa K, Igarashi K: Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity. J Biol Chem. 1998 Jul 10;273(28):17604-9. [9651355 ]

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