Streptavidin

NameStreptavidin
Synonyms
  • Streptavidin precursor
Gene NameNone
OrganismStreptomyces avidinii
Amino acid sequence
>lcl|BSEQ0011189|Streptavidin
MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGAD
GALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQY
VGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDA
VQQ
Number of residuesNone
Molecular Weight18833.61
Theoretical pI8.81
GO Classification
Functions
Processes
Components
  • extracellular region
General FunctionNone
Specific FunctionThe biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP22629
UniProtKB Entry Name
Cellular LocationSecreted
Gene sequence
>lcl|BSEQ0003308|552 bp
ATGCGCAAGATCGTCGTTGCAGCCATCGCCGTTTCCCTGACCACGGTCTCGATTACGGCC
AGCGCTTCGGCAGACCCCTCCAAGGACTCGAAGGCCCAGGTCTCGGCCGCCGAGGCCGGC
ATCACCGGCACCTGGTACAACCAGCTCGGCTCGACCTTCATCGTGACCGCGGGCGCCGAC
GGCGCCCTGACCGGAACCTACGAGTCGGCCGTCGGCAACGCCGAGAGCCGCTACGTCCTG
ACCGGTCGTTACGACAGCGCCCCGGCCACCGACGGCAGCGGCACCGCCCTCGGTTGGACG
GTGGCCTGGAAGAATAACTACCGCAACGCCCACTCCGCGACCACGTGGAGCGGCCAGTAC
GTCGGCGGCGCCGAGGCGAGGATCAACACCCAGTGGCTGCTGACCTCCGGCACCACCGAG
GCCAACGCCTGGAAGTCCACGCTGGTCGGCCACGACACCTTCACCAAGGTGAAGCCGTCC
GCCGCCTCCATCGACGCGGCGAAGAAGGCCGGCGTCAACAACGGCAACCCGCTCGACGCC
GTTCAGCAGTAG
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Argarana CE, Kuntz ID, Birken S, Axel R, Cantor CR: Molecular cloning and nucleotide sequence of the streptavidin gene. Nucleic Acids Res. 1986 Feb 25;14(4):1871-82. [3951999 ]
  2. Gitlin G, Bayer EA, Wilchek M: Studies on the biotin-binding site of streptavidin. Tryptophan residues involved in the active site. Biochem J. 1988 Nov 15;256(1):279-82. [3223904 ]
  3. Gitlin G, Bayer EA, Wilchek M: Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site. Biochem J. 1990 Jul 15;269(2):527-30. [2386489 ]
  4. Alon R, Bayer EA, Wilchek M: Streptavidin contains an RYD sequence which mimics the RGD receptor domain of fibronectin. Biochem Biophys Res Commun. 1990 Aug 16;170(3):1236-41. [2390089 ]
  5. Weber PC, Ohlendorf DH, Wendoloski JJ, Salemme FR: Structural origins of high-affinity biotin binding to streptavidin. Science. 1989 Jan 6;243(4887):85-8. [2911722 ]
  6. Freitag S, Le Trong I, Klumb L, Stayton PS, Stenkamp RE: Structural studies of the streptavidin binding loop. Protein Sci. 1997 Jun;6(6):1157-66. [9194176 ]
  7. Katz BA, Cass RT: In crystals of complexes of streptavidin with peptide ligands containing the HPQ sequence the pKa of the peptide histidine is less than 3.0. J Biol Chem. 1997 May 16;272(20):13220-8. [9148939 ]
  8. Katz BA: Binding of biotin to streptavidin stabilizes intersubunit salt bridges between Asp61 and His87 at low pH. J Mol Biol. 1997 Dec 19;274(5):776-800. [9405158 ]
  9. Freitag S, Le Trong I, Chilkoti A, Klumb LA, Stayton PS, Stenkamp RE: Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. J Mol Biol. 1998 May 29;279(1):211-21. [9636711 ]

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