Molybdopterin synthase catalytic subunit

NameMolybdopterin synthase catalytic subunit
Synonyms
  • 2.8.1.12
  • chlA5
  • Molybdenum cofactor biosynthesis protein E
  • Molybdopterin-converting factor large subunit
  • Molybdopterin-converting factor subunit 2
  • MPT synthase subunit 2
Gene NamemoaE
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0011886|Molybdopterin synthase catalytic subunit
MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLGDSVNALTLEHYPGMTE
KALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYLKT
RAPFWKREATPEGDRWVEARESDQQAAKRW
Number of residuesNone
Molecular Weight16980.955
Theoretical pI5.06
GO Classification
Functions
  • molybdopterin synthase activity
Processes
  • Mo-molybdopterin cofactor biosynthetic process
  • molybdopterin cofactor biosynthetic process
Components
  • cytosol
General FunctionMolybdopterin synthase activity
Specific FunctionConverts molybdopterin precursor Z to molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by MoaD.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP30749
UniProtKB Entry Name
Cellular LocationNone
Gene sequence
>lcl|BSEQ0011887|Molybdopterin synthase catalytic subunit (moaE)
ATGGCAGAAACCAAAATTGTTGTTGGTCCGCAGCCGTTCAGCGTAGGAGAAGAGTACCCG
TGGCTGGCGGAGCGTGACGAAGACGGTGCGGTAGTCACCTTTACTGGTAAGGTGCGCAAC
CATAACCTGGGCGACAGCGTCAACGCATTAACCCTCGAACACTATCCGGGGATGACTGAA
AAAGCACTGGCAGAAATTGTTGATGAAGCGCGTAACCGCTGGCCGCTGGGGCGCGTCACT
GTGATTCACCGCATCGGGGAATTATGGCCGGGCGATGAAATCGTTTTTGTCGGTGTCACC
AGTGCGCATCGCAGCAGTGCGTTTGAAGCCGGGCAGTTTATTATGGATTATCTCAAAACC
CGCGCACCGTTCTGGAAGCGCGAAGCCACGCCGGAAGGCGACCGCTGGGTTGAAGCTCGG
GAGAGCGATCAGCAGGCGGCAAAACGCTGGTAG
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Rivers SL, McNairn E, Blasco F, Giordano G, Boxer DH: Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis. Mol Microbiol. 1993 Jun;8(6):1071-81. [8361352 ]
  2. Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.: A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map. DNA Res. 1996 Jun 30;3(3):137-55. [8905232 ]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [9278503 ]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [16738553 ]
  5. Pitterle DM, Rajagopalan KV: The biosynthesis of molybdopterin in Escherichia coli. Purification and characterization of the converting factor. J Biol Chem. 1993 Jun 25;268(18):13499-505. [8514782 ]
  6. Wuebbens MM, Rajagopalan KV: Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis. J Biol Chem. 2003 Apr 18;278(16):14523-32. Epub 2003 Feb 5. [12571226 ]
  7. Rudolph MJ, Wuebbens MM, Rajagopalan KV, Schindelin H: Crystal structure of molybdopterin synthase and its evolutionary relationship to ubiquitin activation. Nat Struct Biol. 2001 Jan;8(1):42-6. [11135669 ]
  8. Rudolph MJ, Wuebbens MM, Turque O, Rajagopalan KV, Schindelin H: Structural studies of molybdopterin synthase provide insights into its catalytic mechanism. J Biol Chem. 2003 Apr 18;278(16):14514-22. Epub 2003 Feb 5. [12571227 ]

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