Delta-aminolevulinic acid dehydratase

NameDelta-aminolevulinic acid dehydratase
Synonyms
  • 4.2.1.24
  • ALAD
  • Porphobilinogen synthase
Gene NamehemB
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Amino acid sequence
>lcl|BSEQ0010895|Delta-aminolevulinic acid dehydratase
MSFTPANRAYPYTRLRRNRRDDFSRRLVRENVLTVDDLILPVFVLDGVNQRESIPSMPGV
ERLSIDQLLIEAEEWVALGIPALALFPVTPVEKKSLDAAEAYNPEGIAQRATRALRERFP
ELGIITDVALDPFTTHGQDGILDDDGYVLNDVSIDVLVRQALSHAEAGAQVVAPSDMMDG
RIGAIREALESAGHTNVRIMAYSAKYASAYYGPFRDAVGSASNLGKGNKATYQMDPANSD
EALHEVAADLAEGADMVMVKPGMPYLDIVRRVKDEFRAPTFVYQVSGEYAMHMGAIQNGW
LAESVILESLTAFKRAGADGILTYFAKQAAEQLRRGR
Number of residuesNone
Molecular Weight37036.6
Theoretical pI4.83
GO Classification
Functions
  • zinc ion binding
  • porphobilinogen synthase activity
Processes
  • heme biosynthetic process
  • protoporphyrinogen IX biosynthetic process
  • porphyrin-containing compound biosynthetic process
Components
  • cytosol
General FunctionZinc ion binding
Specific FunctionCatalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).
Transmembrane Regions
GenBank Protein ID
UniProtKB IDQ59643
UniProtKB Entry Name
Cellular LocationNone
Gene sequence
>lcl|BSEQ0010896|Delta-aminolevulinic acid dehydratase (hemB)
GTGAGCTTCACTCCCGCCAATCGCGCCTATCCCTACACCCGCCTGCGGCGCAATCGCCGC
GATGACTTCTCCCGCCGCCTGGTCCGCGAGAATGTCCTCACCGTCGACGACCTGATCCTG
CCGGTGTTCGTCCTCGATGGGGTCAACCAGCGTGAAAGCATTCCGTCGATGCCCGGCGTC
GAGCGCCTGTCCATCGACCAGTTGCTGATCGAGGCGGAAGAGTGGGTGGCCCTGGGCATT
CCGGCGCTGGCGCTGTTCCCTGTGACCCCGGTGGAGAAGAAGTCCCTGGACGCCGCCGAG
GCATACAACCCGGAAGGCATCGCCCAGCGCGCCACCCGTGCCCTGCGCGAGCGCTTTCCG
GAGCTGGGCATCATCACCGACGTGGCGCTCGACCCGTTCACCACCCATGGCCAGGACGGC
ATCCTGGATGACGATGGCTACGTCCTCAACGACGTCAGCATCGACGTGCTGGTACGGCAG
GCGCTGTCCCACGCCGAGGCCGGCGCCCAGGTGGTAGCGCCCTCGGACATGATGGACGGT
CGCATCGGTGCGATCCGCGAGGCCCTGGAGTCTGCCGGCCACACCAATGTGCGGATCATG
GCCTACTCGGCCAAGTACGCCAGCGCCTACTACGGTCCGTTCCGCGATGCGGTCGGCTCG
GCTTCGAACCTCGGCAAGGGCAACAAGGCCACCTACCAGATGGATCCGGCGAACAGCGAC
GAGGCACTTCACGAAGTGGCCGCCGACCTGGCCGAAGGCGCCGACATGGTGATGGTCAAG
CCGGGCATGCCCTACCTCGACATCGTGCGTCGGGTGAAGGATGAATTCCGCGCCCCGACC
TTTGTCTACCAGGTCAGCGGCGAGTATGCGATGCACATGGGCGCCATCCAGAACGGCTGG
CTGGCCGAATCGGTGATCCTCGAATCCCTTACCGCCTTCAAACGTGCCGGCGCCGATGGC
ATTCTGACCTACTTCGCCAAGCAGGCCGCAGAACAATTAAGACGGGGGCGTTGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Frankenberg N, Kittel T, Hungerer C, Romling U, Jahn D: Cloning, mapping and functional characterization of the hemB gene of Pseudomonas aeruginosa, which encodes a magnesium-dependent 5-aminolevulinic acid dehydratase. Mol Gen Genet. 1998 Feb;257(4):485-9. [9529530 ]
  2. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [10984043 ]
  3. Frankenberg N, Erskine PT, Cooper JB, Shoolingin-Jordan PM, Jahn D, Heinz DW: High resolution crystal structure of a Mg2+-dependent porphobilinogen synthase. J Mol Biol. 1999 Jun 11;289(3):591-602. [10356331 ]
  4. Frere F, Schubert WD, Stauffer F, Frankenberg N, Neier R, Jahn D, Heinz DW: Structure of porphobilinogen synthase from Pseudomonas aeruginosa in complex with 5-fluorolevulinic acid suggests a double Schiff base mechanism. J Mol Biol. 2002 Jul 5;320(2):237-47. [12079382 ]
  5. Frere F, Nentwich M, Gacond S, Heinz DW, Neier R, Frankenberg-Dinkel N: Probing the active site of Pseudomonas aeruginosa porphobilinogen synthase using newly developed inhibitors. Biochemistry. 2006 Jul 11;45(27):8243-53. [16819823 ]
  6. Heinemann IU, Schulz C, Schubert WD, Heinz DW, Wang YG, Kobayashi Y, Awa Y, Wachi M, Jahn D, Jahn M: Structure of the heme biosynthetic Pseudomonas aeruginosa porphobilinogen synthase in complex with the antibiotic alaremycin. Antimicrob Agents Chemother. 2010 Jan;54(1):267-72. doi: 10.1128/AAC.00553-09. Epub 2009 Oct 12. [19822707 ]

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