Penicillin-insensitive murein endopeptidase

NamePenicillin-insensitive murein endopeptidase
Synonyms
  • 3.4.24.-
  • D-alanyl-D-alanine-endopeptidase
  • DD-endopeptidase
Gene NamemepA
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016643|Penicillin-insensitive murein endopeptidase
MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGCIVGADTLPIQSEHYQV
MRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVD
IFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEIFSLIKLAAQDKDVTRIFVNP
AIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPADSLECEDQPLPPSGDGCGAELQ
SWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI
Number of residuesNone
Molecular Weight30136.415
Theoretical pI8.4
GO Classification
Functions
  • peptidase activity
  • endopeptidase activity
  • serine-type endopeptidase activity
  • metalloendopeptidase activity
  • metal ion binding
Processes
  • response to drug
  • peptidoglycan biosynthetic process
  • peptidoglycan metabolic process
Components
  • outer membrane-bounded periplasmic space
General FunctionSerine-type endopeptidase activity
Specific FunctionInvolved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP0C0T5
UniProtKB Entry Name
Cellular LocationPeriplasm
Gene sequence
>lcl|BSEQ0016644|Penicillin-insensitive murein endopeptidase (mepA)
ATGAATAAAACCGCGATTGCGCTGCTGGCTCTGCTTGCCAGTAGCGCCAGCCTGGCAGCG
ACGCCGTGGCAAAAAATAACCCAACCTGTGCCGGGTAGCGCACAATCGATAGGCAGTTTT
TCTAATGGCTGTATTGTCGGCGCTGACACGCTGCCGATACAGTCCGAACATTATCAGGTC
ATGCGTACCGATCAGCGTCGCTATTTCGGTCACCCGGATCTGGTGATGTTTATCCAGCGT
CTGAGTAGCCAGGTGAGCAATCTGGGCATGGGTACGGTGCTGATTGGCGATATGGGGATG
CCCGCTGGTGGGCGTTTCAACGGCGGTCATGCCAGCCACCAGACCGGACTGGATGTCGAT
ATCTTTCTGCAACTGCCGAAAACTCGCTGGACCTCCGCGCAGCTCTTGCGCCCGCAAGCA
CTGGACTTAGTTTCCCGCGACGGTAAACACGTTGTCTCCACGCTGTGGAAGCCAGAAATT
TTCAGCTTGATCAAACTCGCCGCCCAGGACAAAGACGTCACGCGCATTTTTGTTAATCCG
GCGATTAAACAACAACTTTGCCTTGATGCGGGCACCGATCGCGACTGGTTGCGCAAAGTG
CGACCCTGGTTCCAGCATCGCGCGCATATGCATGTACGATTACGTTGCCCTGCCGATAGT
CTGGAGTGTGAAGATCAACCTTTACCGCCATCAGGCGATGGTTGCGGGGCAGAACTGCAA
AGCTGGTTTGAACCTCCAAAACCGGGAACAACAAAGCCTGAGAAGAAGACACCGCCTCCG
TTGCCGCCTTCCTGCCAGGCGCTACTGGATGAGCACGTGATCTAA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Keck W, van Leeuwen AM, Huber M, Goodell EW: Cloning and characterization of mepA, the structural gene of the penicillin-insensitive murein endopeptidase from Escherichia coli. Mol Microbiol. 1990 Feb;4(2):209-19. [2187143 ]
  2. Yamamoto Y, Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kimura S, Kitagawa M, Makino K, Miki T, Mitsuhashi N, Mizobuchi K, Mori H, Nakade S, Nakamura Y, Nashimoto H, Oshima T, Oyama S, Saito N, Sampei G, Satoh Y, Sivasundaram S, Tagami H, Horiuchi T, et al.: Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features. DNA Res. 1997 Apr 28;4(2):91-113. [9205837 ]
  3. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [9278503 ]
  4. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [16738553 ]
  5. Marcyjaniak M, Odintsov SG, Sabala I, Bochtler M: Peptidoglycan amidase MepA is a LAS metallopeptidase. J Biol Chem. 2004 Oct 15;279(42):43982-9. Epub 2004 Jul 29. [15292190 ]

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