Biphenyl-2,3-diol 1,2-dioxygenase

NameBiphenyl-2,3-diol 1,2-dioxygenase
Synonyms
  • 1.13.11.39
  • 2,3-dihydroxybiphenyl dioxygenase
  • 23OHBP oxygenase
  • DHBD
Gene NamebphC
OrganismBurkholderia xenovorans (strain LB400)
Amino acid sequence
>lcl|BSEQ0010914|Biphenyl-2,3-diol 1,2-dioxygenase
MSIRSLGYMGFAVSDVAAWRSFLTQKLGLMEAGTTDNGDLFRIDSRAWRIAVQQGEVDDL
AFAGYEVADAAGLAQMADKLKQAGIAVTTGDASLARRRGVTGLITFADPFGLPLEIYYGA
SEVFEKPFLPGAAVSGFLTGEQGLGHFVRCVPDSDKALAFYTDVLGFQLSDVIDMKMGPD
VTVPAYFLHCNERHHTLAIAAFPLPKRIHHFMLEVASLDDVGFAFDRVDADGLITSTLGR
HTNDHMVSFYASTPSGVEVEYGWSARTVDRSWVVVRHDSPSMWGHKSVRDKAAARNKA
Number of residuesNone
Molecular Weight32470.515
Theoretical pI5.99
GO Classification
Functions
  • ferrous iron binding
  • biphenyl-2,3-diol 1,2-dioxygenase activity
Processes
  • xenobiotic catabolic process
  • aromatic compound catabolic process
Components
General FunctionFerrous iron binding
Specific FunctionShows a preference for catechols with groups immediately adjacent to the hydroxyl substituents.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP47228
UniProtKB Entry Name
Cellular LocationNone
Gene sequence
>lcl|BSEQ0010915|Biphenyl-2,3-diol 1,2-dioxygenase (bphC)
ATGAGCATCAGAAGTTTGGGATACATGGGGTTTGCGGTCAGCGACGTAGCTGCTTGGCGT
TCGTTTCTGACGCAGAAACTGGGCTTGATGGAAGCGGGCACGACCGACAACGGCGACCTG
TTCCGCATCGATTCGAGAGCCTGGCGGATCGCCGTTCAGCAGGGCGAGGTTGACGATCTG
GCCTTTGCCGGCTACGAGGTGGCCGATGCGGCAGGGCTGGCGCAGATGGCTGACAAGCTC
AAACAGGCCGGTATCGCAGTCACCACCGGCGATGCCTCATTGGCCAGGCGCCGCGGGGTG
ACCGGATTGATCACCTTTGCCGACCCGTTTGGCCTGCCGTTGGAAATTTACTATGGCGCC
AGCGAGGTGTTCGAAAAACCGTTCCTGCCTGGTGCGGCCGTGTCGGGTTTCCTGACCGGC
GAGCAAGGGCTGGGGCATTTCGTGCGCTGCGTTCCGGATTCGGACAAGGCGCTGGCGTTT
TATACCGACGTGCTCGGCTTCCAGTTGTCTGACGTCATCGACATGAAAATGGGGCCGGAC
GTGACGGTTCCTGCGTACTTCCTGCACTGCAACGAACGCCACCACACCCTGGCAATTGCG
GCATTCCCGCTGCCAAAACGCATTCATCACTTCATGCTCGAAGTCGCCTCGCTCGATGAC
GTCGGCTTTGCATTTGATCGGGTTGACGCGGACGGCTTGATCACCTCCACGCTGGGGCGC
CACACCAATGACCACATGGTGTCGTTCTATGCCTCGACCCCGTCCGGAGTAGAGGTCGAG
TATGGCTGGAGTGCCCGTACCGTTGACCGCTCCTGGGTTGTGGTGAGGCACGACAGTCCG
AGCATGTGGGGCCACAAGTCTGTGCGCGACAAAGCAGCTGCGCGCAACAAAGCATGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Hofer B, Eltis LD, Dowling DN, Timmis KN: Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. Gene. 1993 Aug 16;130(1):47-55. [8344527 ]
  2. Chain PS, Denef VJ, Konstantinidis KT, Vergez LM, Agullo L, Reyes VL, Hauser L, Cordova M, Gomez L, Gonzalez M, Land M, Lao V, Larimer F, LiPuma JJ, Mahenthiralingam E, Malfatti SA, Marx CJ, Parnell JJ, Ramette A, Richardson P, Seeger M, Smith D, Spilker T, Sul WJ, Tsoi TV, Ulrich LE, Zhulin IB, Tiedje JM: Burkholderia xenovorans LB400 harbors a multi-replicon, 9.73-Mbp genome shaped for versatility. Proc Natl Acad Sci U S A. 2006 Oct 17;103(42):15280-7. Epub 2006 Oct 9. [17030797 ]
  3. Eltis LD, Hofmann B, Hecht HJ, Lunsdorf H, Timmis KN: Purification and crystallization of 2,3-dihydroxybiphenyl 1,2-dioxygenase. J Biol Chem. 1993 Feb 5;268(4):2727-32. [8428946 ]
  4. Han S, Eltis LD, Timmis KN, Muchmore SW, Bolin JT: Crystal structure of the biphenyl-cleaving extradiol dioxygenase from a PCB-degrading pseudomonad. Science. 1995 Nov 10;270(5238):976-80. [7481800 ]
  5. Vaillancourt FH, Han S, Fortin PD, Bolin JT, Eltis LD: Molecular basis for the stabilization and inhibition of 2, 3-dihydroxybiphenyl 1,2-dioxygenase by t-butanol. J Biol Chem. 1998 Dec 25;273(52):34887-95. [9857017 ]

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