FMN-dependent NADH-azoreductase

NameFMN-dependent NADH-azoreductase
Synonyms
  • 1.7.-.-
  • acpD
  • Azo-dye reductase
  • FMN-dependent NADH-azo compound oxidoreductase
Gene NameazoR
OrganismEscherichia coli (strain K12)
Amino acid sequence
>lcl|BSEQ0016834|FMN-dependent NADH-azoreductase
MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLAANPIPVLDGELVGALR
PSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNISTQLKNYFDLVARAGVTFR
YTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTFLGFIGITDVKFVFAEGIAYG
PEMAAKAQSDAKAAIDSIVSA
Number of residuesNone
Molecular Weight21657.47
Theoretical pI4.84
GO Classification
Functions
  • FMN binding
  • electron carrier activity
  • oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
  • azobenzene reductase activity
  • FMN reductase activity
  • oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor
Processes
  • response to oxidative stress
Components
  • cytosol
General FunctionOxidoreductase activity, acting on nad(p)h, quinone or similar compound as acceptor
Specific FunctionCatalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity. The enzyme can reduce ethyl red and methyl red, but is not able to convert sulfonated azo dyes.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP41407
UniProtKB Entry Name
Cellular LocationNone
Gene sequence
>lcl|BSEQ0016835|FMN-dependent NADH-azoreductase (azoR)
ATGAGCAAGGTATTAGTTCTTAAATCCAGCATCCTGGCAGGGTACTCTCAGTCTAATCAG
TTGTCCGATTATTTTGTTGAACAATGGCGCGAAAAGCACTCCGCTGATGAAATCACCGTT
CGCGACCTGGCTGCAAATCCGATTCCGGTACTGGATGGCGAACTGGTTGGCGCTCTGCGT
CCGAGCGATGCGCCGCTGACTCCGCGTCAGCAGGAAGCTCTGGCACTTTCCGATGAGTTG
ATTGCCGAGCTGAAAGCCCACGACGTTATCGTTATTGCGGCACCGATGTATAACTTCAAC
ATCTCGACTCAGTTGAAAAACTATTTTGACCTGGTTGCCCGCGCAGGCGTTACTTTCCGC
TATACCGAGAACGGTCCGGAAGGTCTGGTAACGGGTAAAAAAGCCATCGTTATTACCAGC
CGCGGCGGGATCCACAAAGATGGACCAACGGACCTGGTGACGCCGTATCTGTCCACGTTC
CTCGGCTTTATCGGCATTACCGATGTGAAATTTGTCTTCGCCGAAGGGATCGCATACGGT
CCGGAAATGGCAGCGAAAGCACAGTCTGACGCAAAAGCAGCCATCGACAGCATTGTTTCT
GCATAA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Aiba H, Baba T, Hayashi K, Inada T, Isono K, Itoh T, Kasai H, Kashimoto K, Kimura S, Kitakawa M, Kitagawa M, Makino K, Miki T, Mizobuchi K, Mori H, Mori T, Motomura K, Nakade S, Nakamura Y, Nashimoto H, Nishio Y, Oshima T, Saito N, Sampei G, Horiuchi T, et al.: A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map. DNA Res. 1996 Dec 31;3(6):363-77. [9097039 ]
  2. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [9278503 ]
  3. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [16738553 ]
  4. Moriya H, Kasai H, Isono K: Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae. Nucleic Acids Res. 1995 Feb 25;23(4):595-8. [7899078 ]
  5. Fischl AS, Kennedy EP: Isolation and properties of acyl carrier protein phosphodiesterase of Escherichia coli. J Bacteriol. 1990 Sep;172(9):5445-9. [2168383 ]
  6. Nakanishi M, Yatome C, Ishida N, Kitade Y: Putative ACP phosphodiesterase gene (acpD) encodes an azoreductase. J Biol Chem. 2001 Dec 7;276(49):46394-9. Epub 2001 Oct 2. [11583992 ]
  7. Ito K, Nakanishi M, Lee WC, Sasaki H, Zenno S, Saigo K, Kitade Y, Tanokura M: Crystallization and preliminary X-ray analysis of AzoR (azoreductase) from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Apr 1;61(Pt 4):399-402. Epub 2005 Mar 24. [16511052 ]

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