Lysozyme

NameLysozyme
Synonyms
  • 4.2.2.n2
  • Lysis protein
  • Lysozyme
  • Muramidase
  • Transglycosylase
Gene NameR
OrganismEnterobacteria phage lambda
Amino acid sequence
>lcl|BSEQ0016637|Endolysin
MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTDYSDHPRKLVTLNPKLK
STGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGALPMIDRGDIRQAIDRC
SNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV
Number of residuesNone
Molecular Weight17825.045
Theoretical pI9.66
GO Classification
Functions
  • lysozyme activity
  • carbon-oxygen lyase activity, acting on polysaccharides
Processes
  • defense response to bacterium
  • cytolysis
  • viral release from host cell
  • peptidoglycan catabolic process
  • cell wall macromolecule catabolic process
Components
  • host cell cytoplasm
General FunctionLysozyme activity
Specific FunctionEndolysin with transglycosylase activity that degrades host peptidoglycans and participates with the holin and spanin proteins in the sequential events which lead to the programmed host cell lysis releasing the mature viral particles. Once the holin has permeabilized the host cell membrane, the endolysin can reach the periplasm and break down the peptidoglycan layer.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP03706
UniProtKB Entry Name
Cellular LocationHost cytoplasm
Gene sequence
>lcl|BSEQ0016638|Endolysin (R)
ATGGTAGAAATCAATAATCAACGTAAGGCGTTCCTCGATATGCTGGCGTGGTCGGAGGGA
ACTGATAACGGACGTCAGAAAACCAGAAATCATGGTTATGACGTCATTGTAGGCGGAGAG
CTATTTACTGATTACTCCGATCACCCTCGCAAACTTGTCACGCTAAACCCAAAACTCAAA
TCAACAGGCGCCGGACGCTACCAGCTTCTTTCCCGTTGGTGGGATGCCTACCGCAAGCAG
CTTGGCCTGAAAGACTTCTCTCCGAAAAGTCAGGACGCTGTGGCATTGCAGCAGATTAAG
GAGCGTGGCGCTTTACCTATGATTGATCGTGGTGATATCCGTCAGGCAATCGACCGTTGC
AGCAATATCTGGGCTTCACTGCCGGGCGCTGGTTATGGTCAGTTCGAGCATAAGGCTGAC
AGCCTGATTGCAAAATTCAAAGAAGCGGGCGGAACGGTCAGAGAGATTGATGTATGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Sanger F, Coulson AR, Hong GF, Hill DF, Petersen GB: Nucleotide sequence of bacteriophage lambda DNA. J Mol Biol. 1982 Dec 25;162(4):729-73. [6221115 ]
  2. Bienkowska-Szewczyk K, Lipinska B, Taylor A: The R gene product of bacteriophage lambda is the murein transglycosylase. Mol Gen Genet. 1981;184(1):111-4. [6460914 ]
  3. Evrard C, Fastrez J, Soumillion P: Histidine modification and mutagenesis point to the involvement of a large conformational change in the mechanism of action of phage lambda lysozyme. FEBS Lett. 1999 Nov 5;460(3):442-6. [10556513 ]
  4. Young R: Phage lysis: do we have the hole story yet? Curr Opin Microbiol. 2013 Dec;16(6):790-7. doi: 10.1016/j.mib.2013.08.008. Epub 2013 Oct 8. [24113139 ]
  5. Evrard C, Fastrez J, Declercq JP: Crystal structure of the lysozyme from bacteriophage lambda and its relationship with V and C-type lysozymes. J Mol Biol. 1998 Feb 13;276(1):151-64. [9514719 ]
  6. Di Paolo A, Duval V, Matagne A, Redfield C: Backbone 1H, 13C, and 15N resonance assignments for lysozyme from bacteriophage lambda. Biomol NMR Assign. 2010 Apr;4(1):111-4. doi: 10.1007/s12104-010-9219-8. Epub 2010 Mar 20. [20300891 ]

From www.t3db.ca