Amylosucrase

Name	Amylosucrase
Synonyms	2.4.1.4
Gene Name	ams
Organism	Neisseria polysaccharea
Amino acid sequence	>lcl\|BSEQ0016901\|Amylosucrase MLTPTQQVGLILQYLKTRILDIYTPEQRAGIEKSEDWRQFSRRMDTHFPKLMNELDSVYG NNEALLPMLEMLLAQAWQSYSQRNSSLKDIDIARENNPDWILSNKQVGGVCYVDLFAGDL KGLKDKIPYFQELGLTYLHLMPLFKCPEGKSDGGYAVSSYRDVNPALGTIGDLREVIAAL HEAGISAVVDFIFNHTSNEHEWAQRCAAGDPLFDNFYYIFPDRRMPDQYDRTLREIFPDQ HPGGFSQLEDGRWVWTTFNSFQWDLNYSNPWVFRAMAGEMLFLANLGVDILRMDAVAFIW KQMGTSCENLPQAHALIRAFNAVMRIAAPAVFFKSEAIVHPDQVVQYIGQDECQIGYNPL QMALLWNTLATREVNLLHQALTYRHNLPEHTAWVNYVRSHDDIGWTFADEDAAYLGISGY DHRQFLNRFFVNRFDGSFARGVPFQYNPSTGDCRVSGTAAALVGLAQDDPHAVDRIKLLY SIALSTGGLPLIYLGDEVGTLNDDDWSQDSNKSDDSRWAHRPRYNEALYAQRNDPSTAAG QIYQGLRHMIAVRQSNPRFDGGRLVTFNTNNKHIIGYIRNNALLAFGNFSEYPQTVTAHT LQAMPFKAHDLIGGKTVSLNQDLTLQPYQVMWLEIA
Number of residues	None
Molecular Weight	72343.005
Theoretical pI	5.55
GO Classification	Functions cation binding amylosucrase activity Processes carbohydrate metabolic process Components extracellular region
General Function	Cation binding
Specific Function	Catalyzes the synthesis of alpha-glucan from sucrose. Catalyzes, in addition, sucrose hydrolysis, maltose and maltotriose synthesis by successive transfers of the glucosyl moiety of sucrose onto the released glucose, and finally turanose and trehalulose synthesis, these two sucrose isomers being obtained by glucosyl transfer onto fructose.
Transmembrane Regions
GenBank Protein ID
UniProtKB ID	Q9ZEU2
UniProtKB Entry Name
Cellular Location	Secreted
Gene sequence	>lcl\|BSEQ0005542\|1911 bp ATGTTGACCCCCACGCAGCAAGTCGGTTTGATTTTACAGTACCTCAAAACACGCATCTTG GACATCTACACGCCCGAACAGCGCGCCGGCATCGAAAAATCCGAAGACTGGCGGCAGTTT TCGCGCCGCATGGATACGCATTTCCCCAAACTGATGAACGAACTCGACAGCGTGTACGGC AACAACGAAGCCCTGCTGCCTATGCTGGAAATGCTGCTGGCGCAGGCATGGCAAAGCTAT TCCCAACGCAACTCATCCTTAAAAGATATCGATATCGCGCGCGAAAACAACCCCGATTGG ATTTTGTCCAACAAACAAGTCGGCGGCGTGTGCTACGTTGATTTGTTTGCCGGCGATTTG AAGGGCTTGAAAGATAAAATTCCTTATTTTCAAGAGCTTGGTTTGACTTATCTGCACCTG ATGCCGCTGTTTAAATGCCCTGAAGGCAAAAGCGACGGCGGCTATGCGGTCAGCAGCTAC CGCGATGTCAATCCGGCACTGGGCACAATAGGCGACTTGCGCGAAGTCATTGCTGCGCTG CACGAAGCCGGCATTTCCGCCGTCGTCGATTTTATCTTCAACCACACCTCCAACGAACAC GAATGGGCGCAACGCTGCGCCGCCGGCGACCCGCTTTTCGACAATTTCTACTATATTTTC CCCGACCGCCGGATGCCCGACCAATACGACCGCACCCTGCGCGAAATCTTCCCCGACCAG CACCCGGGCGGCTTCTCGCAACTGGAAGACGGACGCTGGGTGTGGACGACCTTCAATTCC TTCCAATGGGACTTGAATTACAGCAACCCGTGGGTATTCCGCGCAATGGCGGGCGAAATG CTGTTCCTTGCCAACTTGGGCGTTGACATCCTGCGTATGGATGCGGTTGCCTTTATTTGG AAACAAATGGGGACAAGCTGCGAAAACCTGCCGCAGGCGCACGCCCTCATCCGCGCGTTC AATGCCGTTATGCGTATTGCCGCGCCCGCCGTGTTCTTCAAATCCGAAGCCATCGTCCAC CCCGACCAAGTCGTCCAATACATCGGGCAGGACGAATGCCAAATCGGTTACAACCCCCTG CAAATGGCATTGTTGTGGAACACCCTTGCCACGCGCGAAGTCAACCTGCTCCATCAGGCG CTGACCTACCGCCACAACCTGCCCGAGCATACCGCCTGGGTCAACTACGTCCGCAGCCAC GACGACATCGGCTGGACGTTTGCCGATGAAGACGCGGCATATCTGGGCATAAGCGGCTAC GACCACCGCCAATTCCTCAACCGCTTCTTCGTCAACCGTTTCGACGGCAGCTTCGCTCGT GGCGTACCGTTCCAATACAACCCAAGCACAGGCGACTGCCGTGTCAGTGGTACAGCCGCG GCATTGGTCGGCTTGGCGCAAGACGATCCCCACGCCGTTGACCGCATCAAACTCTTGTAC AGCATTGCTTTGAGTACCGGCGGTCTGCCGCTGATTTACCTAGGCGACGAAGTGGGTACG CTCAATGACGACGACTGGTCGCAAGACAGCAATAAGAGCGACGACAGCCGTTGGGCGCAC CGTCCGCGCTACAACGAAGCCCTGTACGCGCAACGCAACGATCCGTCGACCGCAGCCGGG CAAATCTATCAGGGCTTGCGCCATATGATTGCCGTCCGCCAAAGCAATCCGCGCTTCGAC GGCGGCAGGCTGGTTACATTCAACACCAACAACAAGCACATCATCGGCTACATCCGCAAC AATGCGCTTTTGGCATTCGGTAACTTCAGCGAATATCCGCAAACCGTTACCGCGCATACC CTGCAAGCCATGCCCTTCAAGGCGCACGACCTCATCGGTGGCAAAACTGTCAGCCTGAAT CAGGATTTGACGCTTCAGCCCTATCAGGTCATGTGGCTCGAAATCGCCTGA
GenBank Gene ID
GeneCard ID	None
GenAtlas ID
HGNC ID
Chromosome Location	None
Locus	None
References	Buttcher V, Welsh T, Willmitzer L, Kossmann J: Cloning and characterization of the gene for amylosucrase from Neisseria polysaccharea: production of a linear alpha-1,4-glucan. J Bacteriol. 1997 May;179(10):3324-30. [9150231 ] De Montalk GP, Remaud-Simeon M, Willemot RM, Planchot V, Monsan P: Sequence analysis of the gene encoding amylosucrase from Neisseria polysaccharea and characterization of the recombinant enzyme. J Bacteriol. 1999 Jan;181(2):375-81. [9882648 ] Zhu P, Tsang RS, Tsai CM: Nonencapsulated Neisseria meningitidis strain produces amylopectin from sucrose: altering the concept for differentiation between N. meningitidis and N. polysaccharea. J Clin Microbiol. 2003 Jan;41(1):273-8. [12517860 ] Potocki de Montalk G, Remaud-Simeon M, Willemot RM, Sarcabal P, Planchot V, Monsan P: Amylosucrase from Neisseria polysaccharea: novel catalytic properties. FEBS Lett. 2000 Apr 14;471(2-3):219-23. [10767427 ] Sarcabal P, Remaud-Simeon M, Willemot R, Potocki de Montalk G, Svensson B, Monsan P: Identification of key amino acid residues in Neisseria polysaccharea amylosucrase. FEBS Lett. 2000 May 26;474(1):33-7. [10828446 ] Mirza O, Skov LK, Remaud-Simeon M, Potocki de Montalk G, Albenne C, Monsan P, Gajhede M: Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose. Biochemistry. 2001 Jul 31;40(30):9032-9. [11467966 ] Skov LK, Mirza O, Henriksen A, De Montalk GP, Remaud-Simeon M, Sarcabal P, Willemot RM, Monsan P, Gajhede M: Amylosucrase, a glucan-synthesizing enzyme from the alpha-amylase family. J Biol Chem. 2001 Jul 6;276(27):25273-8. Epub 2001 Apr 16. [11306569 ] Skov LK, Mirza O, Sprogoe D, Dar I, Remaud-Simeon M, Albenne C, Monsan P, Gajhede M: Oligosaccharide and sucrose complexes of amylosucrase. Structural implications for the polymerase activity. J Biol Chem. 2002 Dec 6;277(49):47741-7. Epub 2002 Oct 2. [12364331 ] Jensen MH, Mirza O, Albenne C, Remaud-Simeon M, Monsan P, Gajhede M, Skov LK: Crystal structure of the covalent intermediate of amylosucrase from Neisseria polysaccharea. Biochemistry. 2004 Mar 23;43(11):3104-10. [15023061 ]

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