GDP-mannose 6-dehydrogenase

NameGDP-mannose 6-dehydrogenase
Synonyms
  • 1.1.1.132
  • GMD
Gene NamealgD
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Amino acid sequence
>lcl|BSEQ0019191|GDP-mannose 6-dehydrogenase
MRISIFGLGYVGAVCAGCLSARGHEVIGVDVSSTKIDLINQGKSPIVEPGLEALLQQGRQ
TGRLSGTTDFKKAVLDSDVSFICVGTPSKKNGDLDLGYIETVCREIGFAIREKSERHTVV
VRSTVLPGTVNNVVIPLIEDCSGKKAGVDFGVGTNPEFLRESTAIKDYDFPPMTVIGELD
KQTGDLLEEIYRELDAPIIRKTVEVAEMIKYTCNVWHAAKVTFANEIGNIAKAVGVDGRE
VMDVICQDHKLNLSRYYMRPGFAFGGSCLPKDVRALTYRASQLDVEHPMLGSLMRSNSNQ
VQKAFDLITSHDTRKVGLLGLSFKAGTDDLRESPLVELAEMLIGKGYELRIFDRNVEYAR
VHGANKEYIESKIPHVSSLLVSDLDEVVASSDVLVLGNGDELFVDLVNKTPSGKKLVDLV
GFMPHTTTAQAEGICW
Number of residuesNone
Molecular Weight47599.155
Theoretical pI5.35
GO Classification
Functions
  • NAD binding
  • UDP-glucose 6-dehydrogenase activity
  • GDP-mannose 6-dehydrogenase activity
Processes
  • UDP-glucuronate biosynthetic process
  • alginic acid biosynthetic process
Components
General FunctionUdp-glucose 6-dehydrogenase activity
Specific FunctionCatalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP11759
UniProtKB Entry Name
Cellular LocationNone
Gene sequence
>lcl|BSEQ0019192|GDP-mannose 6-dehydrogenase (algD)
ATGCGAATCAGCATCTTTGGTTTGGGCTATGTCGGTGCAGTATGTGCTGGCTGCCTGTCG
GCACGCGGTCATGAAGTCATTGGTGTGGATGTCTCCAGCACCAAGATCGACCTGATCAAC
CAGGGCAAGTCGCCCATCGTCGAACCGGGCCTGGAAGCGTTGTTGCAGCAAGGCCGGCAG
ACCGGACGGCTGTCGGGCACCACCGACTTCAAGAAGGCCGTGCTGGACTCCGACGTATCG
TTCATCTGCGTCGGCACGCCGAGCAAGAAGAACGGCGACCTGGACCTGGGCTACATCGAG
ACCGTCTGCCGCGAGATCGGCTTCGCCATCCGCGAGAAGTCCGAACGCCACACCGTGGTG
GTGCGCAGCACCGTACTGCCGGGCACCGTCAACAACGTGGTGATCCCGCTGATCGAGGAC
TGCTCGGGCAAGAAGGCCGGGGTCGACTTCGGCGTCGGCACCAACCCCGAATTCCTCCGC
GAGAGCACCGCGATCAAGGACTACGACTTCCCGCCGATGACCGTGATCGGCGAACTGGAC
AAGCAGACCGGCGACCTTCTCGAGGAAATCTACCGCGAGCTGGACGCGCCGATCATCCGC
AAGACCGTCGAGGTCGCCGAGATGATCAAGTACACCTGCAACGTCTGGCACGCCGCCAAG
GTCACCTTCGCCAACGAGATCGGCAACATCGCCAAGGCGGTCGGCGTCGACGGCCGCGAG
GTGATGGACGTGATCTGCCAGGACCACAAGCTCAACCTGTCGCGCTACTACATGCGTCCC
GGCTTCGCCTTCGGCGGCTCCTGCCTGCCCAAGGATGTACGCGCCCTCACCTATCGCGCC
AGCCAGCTGGACGTCGAGCACCCGATGCTCGGTTCGTTGATGCGCAGCAACTCCAACCAG
GTGCAGAAGGCCTTCGATCTCATCACCAGCCACGACACCCGCAAGGTCGGCCTGCTCGGC
CTGTCGTTCAAGGCCGGCACCGACGATTTGCGCGAAAGCCCGCTGGTGGAGCTGGCCGAG
ATGCTCATCGGCAAGGGCTACGAGTTGCGCATCTTCGACCGCAACGTCGAATACGCGCGT
GTCCACGGGGCCAACAAGGAATACATCGAGTCGAAGATCCCGCACGTCTCCTCACTGCTG
GTCTCCGACCTCGACGAAGTGGTGGCGAGTTCCGATGTGCTGGTGCTGGGCAATGGCGAC
GAGCTGTTCGTCGACCTGGTGAACAAGACCCCGAGCGGCAAGAAGCTGGTCGACCTGGTG
GGCTTCATGCCGCACACCACCACTGCCCAGGCCGAGGGCATCTGCTGGTAG
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Deretic V, Gill JF, Chakrabarty AM: Pseudomonas aeruginosa infection in cystic fibrosis: nucleotide sequence and transcriptional regulation of the algD gene. Nucleic Acids Res. 1987 Jun 11;15(11):4567-81. [3108855 ]
  2. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [10984043 ]
  3. Roychoudhury S, May TB, Gill JF, Singh SK, Feingold DS, Chakrabarty AM: Purification and characterization of guanosine diphospho-D-mannose dehydrogenase. A key enzyme in the biosynthesis of alginate by Pseudomonas aeruginosa. J Biol Chem. 1989 Jun 5;264(16):9380-5. [2470755 ]
  4. Roychoudhury S, Chakrabarty K, Ho YK, Chakrabarty AM: Characterization of guanosine diphospho-D-mannose dehydrogenase from Pseudomonas aeruginosa. Structural analysis by limited proteolysis. J Biol Chem. 1992 Jan 15;267(2):990-6. [1370473 ]
  5. Maharaj R, May TB, Wang SK, Chakrabarty AM: Sequence of the alg8 and alg44 genes involved in the synthesis of alginate by Pseudomonas aeruginosa. Gene. 1993 Dec 22;136(1-2):267-9. [8294014 ]
  6. Naught LE, Gilbert S, Imhoff R, Snook C, Beamer L, Tipton P: Allosterism and cooperativity in Pseudomonas aeruginosa GDP-mannose dehydrogenase. Biochemistry. 2002 Jul 30;41(30):9637-45. [12135385 ]
  7. Snook CF, Tipton PA, Beamer LJ: Crystal structure of GDP-mannose dehydrogenase: a key enzyme of alginate biosynthesis in P. aeruginosa. Biochemistry. 2003 Apr 29;42(16):4658-68. [12705829 ]

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