(S)-2-haloacid dehalogenase

Name(S)-2-haloacid dehalogenase
Synonyms
  • 2-haloalkanoic acid dehalogenase
  • 3.8.1.2
  • Halocarboxylic acid halidohydrolase
  • L-2-haloacid dehalogenase
  • L-DEX
Gene NameNone
OrganismPseudomonas sp. (strain YL)
Amino acid sequence
>lcl|BSEQ0011172|(S)-2-haloacid dehalogenase
MDYIKGIAFDLYGTLFDVHSVVGRCDEAFPGRGREISALWRQKQLEYTWLRSLMNRYVNF
QQATEDALRFTCRHLGLDLDARTRSTLCDAYLRLAPFSEVPDSLRELKRRGLKLAILSNG
SPQSIDAVVSHAGLRDGFDHLLSVDPVQVYKPDNRVYELAEQALGLDRSAILFVSSNAWD
ATGARYFGFPTCWINRTGNVFEEMGQTPDWEVTSLRAVVELFETAAGKAEKG
Number of residuesNone
Molecular Weight26176.335
Theoretical pI5.72
GO Classification
Functions
  • (S)-2-haloacid dehalogenase activity
Processes
Components
General Function(s)-2-haloacid dehalogenase activity
Specific FunctionCatalyzes the hydrolytic dehalogenation of small (S)-2-haloalkanoic acids to yield the corresponding (R)-2-hydroxyalkanoic acids. Acts on acids of short chain lengths, C(2) to C(4), with inversion of configuration at C-2.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDQ53464
UniProtKB Entry Name
Cellular LocationCytoplasmic
Gene sequence
>lcl|BSEQ0003266|699 bp
ATGGACTACATCAAGGGTATTGCCTTCGACCTTTACGGTACGCTGTTCGACGTCCATTCG
GTTGTCGGCCGATGCGACGAGGCGTTCCCGGGTCGCGGGCGCGAGATCAGCGCGCTGTGG
CGGCAAAAGCAGCTCGAGTACACATGGCTGCGAAGCCTGATGAATCGCTACGTTAACTTC
CAACAGGCGACCGAAGACGCGTTGCGGTTTACCTGCCGGCATCTTGGGCTCGATCTGGAT
GCGAGAACCCGCAGCACGCTTTGCGACGCGTACTTGCGGCTTGCACCGTTCAGCGAGGTG
CCCGATTCGCTACGCGAACTTAAGCGGCGCGGTCTGAAGCTCGCCATCCTGTCGAACGGC
TCGCCGCAGTCGATCGATGCCGTCGTGAGCCATGCGGGGCTGCGGGATGGATTCGACCAC
CTGCTCAGCGTCGATCCGGTACAGGTCTACAAACCCGATAACCGCGTGTATGAACTCGCG
GAGCAGGCGTTGGGGCTCGACCGATCGGCGATCCTGTTCGTGTCGTCGAACGCGTGGGAC
GCAACGGGGGCGCGCTACTTCGGCTTCCCGACATGCTGGATCAATCGGACCGGCAACGTC
TTTGAAGAGATGGGGCAGACACCCGACTGGGAGGTAACCAGCCTCCGCGCCGTGGTGGAG
CTGTTCGAGACGGCCGCCGGGAAGGCAGAAAAGGGCTGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Nardi-Dei V, Kurihara T, Okamura T, Liu JQ, Koshikawa H, Ozaki H, Terashima Y, Esaki N, Soda K: Comparative studies of genes encoding thermostable L-2-halo acid dehalogenase from Pseudomonas sp. strain YL, other dehalogenases, and two related hypothetical proteins from Escherichia coli. Appl Environ Microbiol. 1994 Sep;60(9):3375-80. [7944368 ]
  2. Kurihara T, Liu JQ, Nardi-Dei V, Koshikawa H, Esaki N, Soda K: Comprehensive site-directed mutagenesis of L-2-halo acid dehalogenase to probe catalytic amino acid residues. J Biochem. 1995 Jun;117(6):1317-22. [7490277 ]
  3. Hisano T, Hata Y, Fujii T, Liu JQ, Kurihara T, Esaki N, Soda K: Crystal structure of L-2-haloacid dehalogenase from Pseudomonas sp. YL. An alpha/beta hydrolase structure that is different from the alpha/beta hydrolase fold. J Biol Chem. 1996 Aug 23;271(34):20322-30. [8702766 ]
  4. Li YF, Hata Y, Fujii T, Hisano T, Nishihara M, Kurihara T, Esaki N: Crystal structures of reaction intermediates of L-2-haloacid dehalogenase and implications for the reaction mechanism. J Biol Chem. 1998 Jun 12;273(24):15035-44. [9614112 ]

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