Steroid Delta-isomerase

NameSteroid Delta-isomerase
Synonyms
  • 5.3.3.1
  • Delta(5)-3-ketosteroid isomerase
Gene Nameksi
OrganismPseudomonas putida
Amino acid sequence
>lcl|BSEQ0010861|Steroid Delta-isomerase
MNLPTAQEVQGLMARYIELVDVGDIEAIVQMYADDATVEDPFGQPPIHGREQIAAFYRQG
LGGGKVRACLTGPVRASHNGCGAMPFRVEMVWNGQPCALDVIDVMRFDEHGRIQTMQAYW
SEVNLSVREPQ
Number of residuesNone
Molecular Weight14535.48
Theoretical pI4.53
GO Classification
Functions
  • steroid delta-isomerase activity
Processes
  • steroid metabolic process
Components
General FunctionSteroid delta-isomerase activity
Specific FunctionNone
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP07445
UniProtKB Entry Name
Cellular LocationNone
Gene sequence
>lcl|BSEQ0002460|396 bp
ATGAACCTACCGACTGCGCAGGAAGTCCAGGGCCTGATGGCCCGTTACATCGAGCTGGTC
GATGTCGGGGATATCGAGGCGATCGTGCAGATGTACGCCGATGACGCCACGGTCGAAGAC
CCGTTTGGCCAGCCGCCGATCCACGGCCGCGAGCAGATTGCCGCGTTCTATCGCCAGGGT
TTGGGCGGGGGCAAGGTCCGCGCCTGCCTGACCGGGCCGGTACGGGCCAGCCATAACGGC
TGCGGGGCGATGCCGTTTCGCGTCGAGATGGTCTGGAACGGCCAGCCCTGTGCACTGGAT
GTCATCGATGTGATGCGCTTTGATGAGCACGGCCGGATCCAGACGATGCAAGCCTACTGG
AGCGAGGTCAACCTCAGCGTGCGCGAGCCGCAGTAG
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Kim SW, Kim CY, Benisek WF, Choi KY: Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B. J Bacteriol. 1994 Nov;176(21):6672-6. [7961420 ]
  2. Linden KG, Benisek WF: The amino acid sequence of a delta 5-3-oxosteroid isomerase from Pseudomonas putida biotype B. J Biol Chem. 1986 May 15;261(14):6454-60. [3700400 ]
  3. Kim SW, Cha SS, Cho HS, Kim JS, Ha NC, Cho MJ, Joo S, Kim KK, Choi KY, Oh BH: High-resolution crystal structures of delta5-3-ketosteroid isomerase with and without a reaction intermediate analogue. Biochemistry. 1997 Nov 18;36(46):14030-6. [9369474 ]
  4. Kim DH, Nam GH, Jang DS, Choi G, Joo S, Kim JS, Oh BH, Choi KY: Roles of active site aromatic residues in catalysis by ketosteroid isomerase from Pseudomonas putida biotype B. Biochemistry. 1999 Oct 19;38(42):13810-9. [10529226 ]
  5. Choi G, Ha NC, Kim SW, Kim DH, Park S, Oh BH, Choi KY: Asp-99 donates a hydrogen bond not to Tyr-14 but to the steroid directly in the catalytic mechanism of Delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B. Biochemistry. 2000 Feb 8;39(5):903-9. [10653633 ]
  6. Ha NC, Kim MS, Lee W, Choi KY, Oh BH: Detection of large pKa perturbations of an inhibitor and a catalytic group at an enzyme active site, a mechanistic basis for catalytic power of many enzymes. J Biol Chem. 2000 Dec 29;275(52):41100-6. [11007792 ]
  7. Nam GH, Jang DS, Cha SS, Lee TH, Kim DH, Hong BH, Yun YS, Oh BH, Choi KY: Maintenance of alpha-helical structures by phenyl rings in the active-site tyrosine triad contributes to catalysis and stability of ketosteroid isomerase from Pseudomonas putida biotype B. Biochemistry. 2001 Nov 13;40(45):13529-37. [11695900 ]
  8. Yun YS, Nam GH, Kim YG, Oh BH, Choi KY: Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B. FEBS J. 2005 Apr;272(8):1999-2011. [15819891 ]

From www.t3db.ca