Cocaine esterase

Name	Cocaine esterase
Synonyms	3.1.1.84
Gene Name	cocE
Organism	Rhodococcus sp. (strain MB1 Bresler)
Amino acid sequence	>lcl\|BSEQ0010990\|Cocaine esterase MVDGNYSVASNVMVPMRDGVRLAVDLYRPDADGPVPVLLVRNPYDKFDVFAWSTQSTNWL EFVRDGYAVVIQDTRGLFASEGEFVPHVDDEADAEDTLSWILEQAWCDGNVGMFGVSYLG VTQWQAAVSGVGGLKAIAPSMASADLYRAPWYGPGGALSVEALLGWSALIGTGLITSRSD ARPEDAADFVQLAAILNDVAGAASVTPLAEQPLLGRLIPWVIDQVVDHPDNDESWQSISL FERLGGLATPALITAGWYDGFVGESLRTFVAVKDNADARLVVGPWSHSNLTGRNADRKFG IAATYPIQEATTMHKAFFDRHLRGETDALAGVPKVRLFVMGIDEWRDETDWPLPDTAYTP FYLGGSGAANTSTGGGTLSTSISGTESADTYLYDPADPVPSLGGTLLFHNGDNGPADQRP IHDRDDVLCYSTEVLTDPVEVTGTVSARLFVSSSAVDTDFTAKLVDVFPDGRAIALCDGI VRMRYRETLVNPTLIEAGEIYEVAIDMLATSNVFLPGHRIMVQVSSSNFPKYDRNSNTGG VIAREQLEEMCTAVNRIHRGPEHPSHIVLPIIKR
Number of residues	None
Molecular Weight	62131.125
Theoretical pI	4.31
GO Classification	Functions carboxylic ester hydrolase activity dipeptidyl-peptidase activity Processes cocaine catabolic process Components cytoplasm
General Function	Dipeptidyl-peptidase activity
Specific Function	Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants. Also efficiently hydrolyzes cocaethylene, a more potent cocaine metabolite that has been observed in patients who concurrently abuse cocaine and alcohol. Is able to prevent cocaine-induced convulsions and lethality in rat.
Transmembrane Regions
GenBank Protein ID
UniProtKB ID	Q9L9D7
UniProtKB Entry Name
Cellular Location	Cytoplasm
Gene sequence	>lcl\|BSEQ0002804\|1725 bp ATGGTGGACGGGAATTACAGTGTTGCCTCGAACGTGATGGTTCCGATGCGTGATGGGGTG CGTCTGGCGGTCGACCTGTACCGACCAGATGCTGATGGACCTGTTCCGGTCCTGCTGGTT CGCAACCCATACGACAAGTTCGACGTGTTCGCGTGGTCGACGCAGTCGACAAACTGGCTT GAGTTCGTGCGTGATGGCTATGCCGTGGTCATTCAAGACACGCGTGGCTTGTTCGCATCG GAAGGTGAGTTCGTCCCGCACGTTGACGACGAAGCTGACGCCGAGGATACGTTGAGCTGG ATTCTGGAACAAGCGTGGTGCGACGGCAATGTGGGCATGTTCGGCGTTTCGTACTTGGGT GTGACCCAGTGGCAGGCCGCCGTATCCGGCGTTGGTGGGCTGAAGGCGATCGCGCCGTCC ATGGCGTCGGCGGACTTGTACCGCGCCCCGTGGTACGGCCCTGGTGGTGCGCTTTCAGTC GAGGCGCTGTTGGGCTGGTCAGCTCTCATAGGTACTGGGCTCATCACGTCGAGGTCTGAC GCCCGGCCCGAAGACGCAGCCGACTTCGTCCAACTCGCAGCAATTCTCAATGACGTCGCT GGCGCGGCGTCGGTCACGCCCCTGGCCGAGCAACCGCTTCTGGGCCGACTGATTCCGTGG GTGATCGATCAGGTTGTCGATCACCCCGACAACGATGAATCATGGCAGTCCATTAGCTTG TTTGAACGACTCGGCGGGTTGGCAACACCGGCCTTGATCACGGCTGGGTGGTACGACGGG TTCGTCGGCGAATCGTTGCGCACTTTCGTTGCGGTCAAGGACAATGCCGACGCACGTTTG GTTGTCGGCCCTTGGAGTCACAGCAACCTCACTGGTCGGAATGCGGACCGGAAGTTCGGC ATTGCCGCGACCTACCCGATTCAAGAAGCCACCACGATGCACAAGGCATTCTTCGACCGG CACCTCCGCGGCGAGACCGATGCACTCGCAGGCGTCCCCAAAGTGCGGCTGTTCGTAATG GGCATCGATGAGTGGCGTGACGAAACGGACTGGCCACTGCCGGACACGGCGTATACGCCC TTCTATCTTGGAGGTAGCGGGGCTGCGAATACCTCCACGGGTGGTGGAACACTGTCGACG TCGATTTCCGGAACTGAATCTGCTGACACCTACCTGTATGATCCGGCCGATCCCGTGCCT TCGCTCGGGGGGACGCTGCTGTTCCACAACGGAGACAACGGACCCGCCGACCAACGTCCC ATTCATGACCGGGACGACGTGTTGTGTTACAGCACTGAGGTATTGACCGACCCGGTGGAA GTAACCGGCACCGTCTCCGCCCGGCTGTTCGTGTCGTCATCAGCGGTGGACACTGATTTC ACCGCCAAACTTGTCGACGTATTTCCCGACGGTCGCGCGATCGCGCTGTGTGACGGGATC GTGCGGATGCGGTACCGCGAGACGTTGGTCAATCCAACCTTGATCGAAGCGGGCGAAATC TACGAGGTTGCTATCGACATGCTTGCAACCTCGAATGTATTCCTGCCAGGGCATCGCATC ATGGTCCAAGTATCAAGTAGCAACTTCCCGAAATACGACCGCAATTCGAATACCGGCGGA GTAATCGCACGGGAACAGCTCGAAGAGATGTGCACCGCCGTGAACCGCATTCACCGAGGA CCTGAGCATCCCAGCCACATTGTGCTGCCGATTATCAAGCGATAG
GenBank Gene ID
GeneCard ID	None
GenAtlas ID
HGNC ID
Chromosome Location	None
Locus	None
References	Bresler MM, Rosser SJ, Basran A, Bruce NC: Gene cloning and nucleotide sequencing and properties of a cocaine esterase from Rhodococcus sp. strain MB1. Appl Environ Microbiol. 2000 Mar;66(3):904-8. [10698749 ] Cooper ZD, Narasimhan D, Sunahara RK, Mierzejewski P, Jutkiewicz EM, Larsen NA, Wilson IA, Landry DW, Woods JH: Rapid and robust protection against cocaine-induced lethality in rats by the bacterial cocaine esterase. Mol Pharmacol. 2006 Dec;70(6):1885-91. Epub 2006 Sep 12. [16968810 ] Larsen NA, Turner JM, Stevens J, Rosser SJ, Basran A, Lerner RA, Bruce NC, Wilson IA: Crystal structure of a bacterial cocaine esterase. Nat Struct Biol. 2002 Jan;9(1):17-21. [11742345 ] Turner JM, Larsen NA, Basran A, Barbas CF 3rd, Bruce NC, Wilson IA, Lerner RA: Biochemical characterization and structural analysis of a highly proficient cocaine esterase. Biochemistry. 2002 Oct 15;41(41):12297-307. [12369817 ] Narasimhan D, Nance MR, Gao D, Ko MC, Macdonald J, Tamburi P, Yoon D, Landry DM, Woods JH, Zhan CG, Tesmer JJ, Sunahara RK: Structural analysis of thermostabilizing mutations of cocaine esterase. Protein Eng Des Sel. 2010 Jul;23(7):537-47. doi: 10.1093/protein/gzq025. Epub 2010 Apr 30. [20436035 ] Narasimhan D, Collins GT, Nance MR, Nichols J, Edwald E, Chan J, Ko MC, Woods JH, Tesmer JJ, Sunahara RK: Subunit stabilization and polyethylene glycolation of cocaine esterase improves in vivo residence time. Mol Pharmacol. 2011 Dec;80(6):1056-65. doi: 10.1124/mol.111.074997. Epub 2011 Sep 2. [21890748 ]

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