Neopullulanase 2

Name	Neopullulanase 2
Synonyms	3.2.1.135 Alpha-amylase II TVA II
Gene Name	tvaII
Organism	Thermoactinomyces vulgaris
Amino acid sequence	>lcl\|BSEQ0011001\|Neopullulanase 2 MLLEAIFHEAKGSYAYPISETQLRVRLRAKKGDVVRCEVLYADRYASPEEELAHALAGKA GSDERFDYFEALLECSTKRVKYVFLLTGPQGEAVYFGETGFSAERSKAGVFQYAYIHRSE VFTTPEWAKEAVIYQIFPERFANGDPSNDPPGTEQWAKDARPRHDSFYGGDLKGVIDRLP YLEELGVTALYFTPIFASPSHHKYDTADYLAIDPQFGDLPTFRRLVDEAHRRGIKIILDA VFNHAGDQFFAFRDVLQKGEQSRYKDWFFIEDFPVSKTSRTNYETFAVQVPAMPKLRTEN PEVKEYLFDVARFWMEQGIDGWRLDVANEVDHAFWREFRRLVKSLNPDALIVGEIWHDAS GWLMGDQFDSVMNYLFRESVIRFFATGEIHAERFDAELTRARMLYPEQAAQGLWNLLDSH DTERFLTSCGGNEAKFRLAVLFQMTYLGTPLIYYGDEIGMAGATDPDCRRPMIWEEKEQN RGLFEFYKELIRLRHRLASLTRGNVRSWHADKQANLYAFVRTVQDQHVGVVLNNRGEKQT VLLQVPESGGKTWLDCLTGEEVHGKQGQLKLTLRPYQGMILWNGR
Number of residues	None
Molecular Weight	67466.71
Theoretical pI	5.89
GO Classification	Functions metal ion binding neopullulanase activity Processes carbohydrate metabolic process Components
General Function	Neopullulanase activity
Specific Function	Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose.
Transmembrane Regions
GenBank Protein ID
UniProtKB ID	Q08751
UniProtKB Entry Name
Cellular Location	Cytoplasmic
Gene sequence	>lcl\|BSEQ0002832\|1758 bp ATGCTGTTGGAAGCGATTTTTCATGAAGCGAAAGGAAGCTATGCTTACCCGATTTCGGAA ACGCAACTGCGGGTGAGATTGCGGGCGAAAAAAGGGGACGTGGTGAGATGCGAGGTGCTC TATGCGGACCGCTATGCTTCGCCGGAGGAAGAACTGGCACATGCACTTGCGGGCAAAGCA GGGTCCGATGAGCGATTTGATTATTTTGAGGCTTTACTGGAATGTTCCACCAAACGGGTG AAATACGTGTTTTTGTTGACAGGCCCACAAGGGGAGGCTGTTTATTTTGGCGAGACCGGT TTTTCCGCCGAACGGTCTAAAGCGGGGGTGTTCCAATATGCTTACATCCACCGGAGCGAA GTGTTTACGACACCGGAATGGGCAAAGGAGGCGGTGATCTACCAGATCTTTCCCGAACGT TTTGCCAATGGGGATCCCAGCAATGATCCGCCGGGAACGGAACAATGGGCCAAAGACGCC AGACCTCGGCACGACAGTTTTTACGGCGGAGATTTAAAGGGGGTGATCGACCGTTTGCCA TATTTGGAGGAGTTGGGAGTGACGGCGCTTTATTTTACTCCCATTTTTGCTTCGCCGTCC CATCACAAATATGATACAGCTGATTATTTGGCGATTGATCCGCAATTTGGGGATTTGCCC ACCTTTCGGCGTTTGGTGGATGAAGCTCACCGGCGCGGGATCAAAATCATTTTGGATGCC GTGTTTAACCATGCCGGAGATCAGTTTTTTGCGTTTCGGGATGTTCTGCAAAAGGGAGAA CAGTCGCGATATAAGGATTGGTTTTTCATCGAGGATTTTCCCGTTTCCAAGACGTCAAGA ACCAATTATGAAACCTTTGCCGTCCAAGTGCCGGCCATGCCCAAATTGCGGACGGAAAAT CCGGAAGTGAAGGAATATTTGTTTGATGTCGCGCGATTTTGGATGGAGCAGGGGATCGAC GGATGGAGACTGGATGTGGCCAATGAGGTGGATCACGCCTTTTGGCGCGAGTTTCGCCGC CTTGTCAAATCCCTCAATCCCGACGCCTTGATTGTCGGAGAGATTTGGCATGACGCTTCC GGCTGGCTGATGGGAGACCAATTTGATTCGGTGATGAATTATTTGTTTCGCGAGAGTGTG ATCCGTTTTTTTGCCACGGGGGAGATCCATGCCGAAAGATTTGACGCCGAACTTACCCGG GCCAGGATGCTTTATCCGGAACAGGCGGCACAAGGGTTATGGAACCTCCTCGACTCACAC GATACGGAACGTTTTTTGACATCGTGTGGGGGGAATGAGGCGAAATTCCGCCTGGCTGTT TTGTTTCAGATGACATACCTGGGGACGCCGCTGATTTATTACGGTGATGAGATCGGGATG GCCGGAGCGACGGACCCGGATTGCCGGCGCCCGATGATATGGGAGGAAAAAGAGCAGAAC CGCGGCTTGTTTGAGTTTTATAAGGAATTGATTCGCTTGCGCCATCGGCTGGCATCGCTC ACCAGGGGAAATGTCCGGTCTTGGCATGCCGACAAACAAGCCAATCTTTATGCTTTTGTG CGCACGGTTCAAGACCAACATGTCGGTGTGGTTCTCAATAATCGTGGCGAGAAGCAAACC GTTTTGCTACAGGTGCCGGAATCGGGCGGAAAAACTTGGCTGGATTGTTTGACCGGAGAA GAAGTGCATGGCAAACAAGGGCAACTGAAGCTGACACTTCGCCCTTACCAGGGAATGATT CTCTGGAATGGCCGGTAG
GenBank Gene ID
GeneCard ID	None
GenAtlas ID
HGNC ID
Chromosome Location	None
Locus	None
References	Tonozuka T, Ohtsuka M, Mogi S, Sakai H, Ohta T, Sakano Y: A neopullulanase-type alpha-amylase gene from Thermoactinomyces vulgaris R-47. Biosci Biotechnol Biochem. 1993 Mar;57(3):395-401. [7763540 ] Kamitori S, Kondo S, Okuyama K, Yokota T, Shimura Y, Tonozuka T, Sakano Y: Crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVAII) hydrolyzing cyclodextrins and pullulan at 2.6 A resolution. J Mol Biol. 1999 Apr 16;287(5):907-21. [10222200 ] Kamitori S, Abe A, Ohtaki A, Kaji A, Tonozuka T, Sakano Y: Crystal structures and structural comparison of Thermoactinomyces vulgaris R-47 alpha-amylase 1 (TVAI) at 1.6 A resolution and alpha-amylase 2 (TVAII) at 2.3 A resolution. J Mol Biol. 2002 Apr 26;318(2):443-53. [12051850 ]

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