Deacetoxycephalosporin C synthase

Name	Deacetoxycephalosporin C synthase
Synonyms	1.14.20.1 DAOCS Expandase
Gene Name	cefE
Organism	Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
Amino acid sequence	>lcl\|BSEQ0011313\|Deacetoxycephalosporin C synthase MDTTVPTFSLAELQQGLHQDEFRRCLRDKGLFYLTDCGLTDTELKSAKDLVIDFFEHGSE AEKRAVTSPVPTMRRGFTGLESESTAQITNTGSYSDYSMCYSMGTADNLFPSGDFERIWT QYFDRQYTASRAVAREVLRATGTEPDGGVEAFLDCEPLLRFRYFPQVPEHRSAEEQPLRM APHYDLSMVTLIQQTPCANGFVSLQAEVGGAFTDLPYRPDAVLVFCGAIATLVTGGQVKA PRHHVAAPRRDQIAGSSRTSSVFFLRPNADFTFSVPLARECGFDVSLDGETATFQDWIGG NYVNIRRTSKA
Number of residues	None
Molecular Weight	34555.38
Theoretical pI	4.94
GO Classification	Functions iron ion binding L-ascorbic acid binding deacetoxycephalosporin-C synthase activity Processes antibiotic biosynthetic process Components
General Function	L-ascorbic acid binding
Specific Function	Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.
Transmembrane Regions
GenBank Protein ID
UniProtKB ID	P18548
UniProtKB Entry Name
Cellular Location	None
Gene sequence	>lcl\|BSEQ0003631\|936 bp ATGGACACGACGGTGCCCACCTTCAGCCTGGCCGAACTCCAGCAGGGCCTGCACCAGGAC GAGTTCCGCAGGTGTCTGAGGGACAAGGGCCTCTTCTATCTGACGGACTGCGGTCTGACC GACACCGAGCTGAAGTCGGCCAAGGACCTCGTCATCGACTTCTTCGAGCACGGCAGCGAG GCGGAGAAGCGCGCCGTCACCTCGCCCGTCCCCACCATGCGCCGCGGCTTCACCGGGCTG GAGTCGGAGAGCACCGCCCAGATCACCAATACCGGCAGCTACTCCGACTACTCGATGTGC TACTCGATGGGCACCGCGGACAACCTCTTCCCGTCCGGTGACTTCGAGCGGATCTGGACC CAGTACTTCGACCGCCAGTACACCGCCTCCCGCGCGGTCGCCCGGGAGGTCCTGCGGGCG ACCGGGACCGAGCCCGACGGCGGGGTCGAGGCCTTCCTCGACTGCGAGCCGCTGCTGCGG TTCCGCTACTTCCCGCAGGTCCCCGAGCACCGCAGCGCCGAGGAGCAGCCCCTGCGGATG GCGCCGCACTACGACCTGTCGATGGTCACCCTCATCCAGCAGACACCCTGCGCCAACGGC TTCGTCAGCCTCCAGGCCGAGGTCGGCGGCGCGTTCACGGACCTGCCCTACCGTCCGGAC GCCGTCCTCGTCTTCTGCGGCGCCATCGCGACCCTGGTGACCGGCGGCCAGGTCAAGGCC CCCCGGCACCATGTCGCGGCCCCCCGCAGGGACCAGATAGCGGGCAGCAGCCGCACCTCC AGTGTGTTCTTCCTCCGTCCCAACGCGGACTTCACCTTCTCCGTCCCGCTGGCGCGCGAG TGCGGCTTCGATGTCAGCCTGGACGGCGAGACCGCCACGTTCCAGGATTGGATCGGGGGC AACTACGTGAACATCCGCCGCACATCCAAGGCATAG
GenBank Gene ID
GeneCard ID	None
GenAtlas ID
HGNC ID
Chromosome Location	None
Locus	None
References	Kovacevic S, Weigel BJ, Tobin MB, Ingolia TD, Miller JR: Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase. J Bacteriol. 1989 Feb;171(2):754-60. [2644235 ] Kovacevic S, Tobin MB, Miller JR: The beta-lactam biosynthesis genes for isopenicillin N epimerase and deacetoxycephalosporin C synthetase are expressed from a single transcript in Streptomyces clavuligerus. J Bacteriol. 1990 Jul;172(7):3952-8. [1694525 ]

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