2-isopropylmalate synthase

Name	2-isopropylmalate synthase
Synonyms	2.3.3.13 Alpha-IPM synthase Alpha-isopropylmalate synthase
Gene Name	leuA
Organism	Mycobacterium tuberculosis
Amino acid sequence	>lcl\|BSEQ0051241\|2-isopropylmalate synthase MTTSESPDAYTESFGAHTIVKPAGPPRVGQPSWNPQRASSMPVNRYRPFAEEVEPIRLRN RTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDLLVRMGYKEIEVGFPSASQT DFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGAPRAIVHFYNSTSILQRRVV FRANRAEVQAIATDGARKCVEQAAKYPGTQWRFEYSPESYTGTELEYAKQVCDAVGEVIA PTPERPIIFNLPATVEMTTPNVYADSIEWMSRNLANRESVILSLHPHNDRGTAVAAAELG FAAGADRIEGCLFGNGERTGNVCLVTLGLNLFSRGVDPQIDFSNIDEIRRTVEYCNQLPV HERHPYGGDLVYTAFSGSHQDAINKGLDAMKLDADAADCDVDDMLWQVPYLPIDPRDVGR TYEAVIRVNSQSGKGGVAYIMKTDHGLSLPRRLQIEFSQVIQKIAEGTAGEGGEVSPKEM WDAFAEEYLAPVRPLERIRQHVDAADDDGGTTSITATVKINGVETEISGSGNGPLAAFVH ALADVGFDVAVLDYYEHAMSAGDDAQAAAYVEASVTIASPAQPGEAGRHASDPVTIASPA QPGEAGRHASDPVTSKTVWGVGIAPSITTASLRAVVSAVNRAAR
Number of residues	None
Molecular Weight	70112.945
Theoretical pI	None
GO Classification	Functions zinc ion binding manganese ion binding magnesium ion binding 2-isopropylmalate synthase activity potassium ion binding Processes growth leucine biosynthetic process Components extracellular region plasma membrane
General Function	Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Specific Function	2-isopropylmalate synthase activity
Transmembrane Regions
GenBank Protein ID
UniProtKB ID	P9WQB3
UniProtKB Entry Name
Cellular Location	None
Gene sequence	>lcl\|BSEQ0051242\|2-isopropylmalate synthase (leuA) GTGACAACTTCTGAATCGCCCGACGCCTATACCGAGTCGTTTGGGGCCCACACCATCGTG AAACCCGCCGGCCCACCTCGCGTCGGTCAGCCCTCGTGGAATCCGCAGCGAGCCTCGTCG ATGCCGGTCAACCGCTACCGGCCGTTCGCCGAGGAGGTCGAGCCCATCCGGCTGAGAAAC CGCACGTGGCCTGATCGCGTCATCGATCGTGCGCCGCTGTGGTGCGCGGTCGACTTACGC GATGGCAACCAGGCGCTGATCGACCCGATGAGCCCGGCCCGCAAGCGCCGCATGTTCGAC CTGCTGGTCCGGATGGGCTACAAGGAGATTGAGGTGGGGTTCCCCTCGGCCAGCCAGACC GACTTCGACTTCGTCAGAGAGATCATCGAGCAGGGCGCCATTCCCGACGACGTCACCATC CAGGTGCTCACCCAATGCCGTCCCGAGCTGATCGAGCGCACCTTCCAGGCGTGTTCGGGC GCACCCCGGGCCATCGTGCACTTCTACAACTCGACGTCAATCCTGCAGCGCCGCGTGGTC TTTCGCGCCAACCGGGCTGAGGTGCAGGCCATCGCGACAGATGGGGCGCGCAAGTGCGTC GAGCAGGCCGCCAAATACCCGGGCACGCAGTGGCGATTCGAGTACTCCCCGGAGTCCTAC ACCGGCACCGAACTGGAATACGCCAAACAGGTGTGCGACGCCGTCGGCGAGGTCATTGCG CCGACGCCGGAGCGCCCGATCATCTTCAACCTGCCCGCCACGGTGGAGATGACGACGCCC AATGTCTACGCCGACTCGATCGAGTGGATGAGCCGCAACCTAGCCAACCGGGAGTCGGTC ATCCTGAGCCTGCACCCGCACAATGACCGCGGAACCGCCGTCGCCGCAGCGGAATTGGGT TTCGCGGCCGGGGCTGATCGGATCGAGGGCTGCCTGTTCGGCAACGGCGAGCGCACCGGC AACGTGTGCCTGGTCACGCTGGGACTCAACCTGTTCTCCCGAGGTGTGGACCCGCAGATC GACTTCTCCAATATTGACGAGATCCGGCGCACGGTGGAGTACTGCAACCAGCTGCCGGTG CACGAACGTCACCCCTATGGCGGCGACCTGGTGTACACCGCGTTCTCCGGTAGCCACCAG GACGCCATCAACAAGGGCCTAGACGCGATGAAGCTGGATGCGGATGCCGCCGACTGTGAC GTCGACGACATGCTGTGGCAGGTGCCGTATCTGCCCATCGACCCGCGCGATGTCGGGCGC ACCTACGAGGCGGTGATCCGGGTCAACTCGCAGTCCGGCAAGGGCGGCGTGGCCTACATC ATGAAGACCGACCACGGCCTTTCCCTGCCGCGGCGGCTGCAGATCGAGTTTTCCCAGGTA ATCCAGAAGATCGCAGAGGGTACAGCAGGCGAGGGTGGCGAGGTCTCGCCCAAGGAGATG TGGGATGCGTTCGCCGAGGAGTATCTGGCCCCGGTGCGGCCTTTGGAGCGGATAAGGCAA CATGTGGACGCTGCCGACGACGACGGCGGCACGACCAGCATCACGGCGACCGTCAAGATC AACGGCGTGGAGACCGAGATCAGCGGGTCCGGTAACGGTCCGTTGGCCGCGTTCGTCCAT GCGCTGGCCGATGTCGGGTTTGACGTGGCCGTGCTGGACTACTACGAGCACGCGATGAGC GCCGGCGACGACGCTCAGGCCGCCGCGTATGTGGAGGCCTCCGTGACGATCGCGAGCCCG GCGCAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGATCGCGAGCCCGGCG CAGCCGGGCGAAGCGGGTCGGCACGCATCGGACCCCGTGACGAGTAAGACGGTGTGGGGT GTCGGTATCGCACCGTCAATCACCACCGCGTCGCTGCGCGCCGTGGTGTCGGCGGTCAAC CGGGCGGCACGCTAG
GenBank Gene ID
GeneCard ID	None
GenAtlas ID
HGNC ID
Chromosome Location	None
Locus	None
References	Chanchaem W, Palittapongarnpim P: A variable number of tandem repeats result in polymorphic alpha -isopropylmalate synthase in Mycobacterium tuberculosis. Tuberculosis (Edinb). 2002;82(1):1-6. [11914056 ] Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE 3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, Barrell BG: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature. 1998 Jun 11;393(6685):537-44. [9634230 ] Raman K, Yeturu K, Chandra N: targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis. BMC Syst Biol. 2008 Dec 19;2:109. doi: 10.1186/1752-0509-2-109. [19099550 ] Kelkar DS, Kumar D, Kumar P, Balakrishnan L, Muthusamy B, Yadav AK, Shrivastava P, Marimuthu A, Anand S, Sundaram H, Kingsbury R, Harsha HC, Nair B, Prasad TS, Chauhan DS, Katoch K, Katoch VM, Kumar P, Chaerkady R, Ramachandran S, Dash D, Pandey A: Proteogenomic analysis of Mycobacterium tuberculosis by high resolution mass spectrometry. Mol Cell Proteomics. 2011 Dec;10(12):M111.011627. doi: 10.1074/mcp.M111.011445. Epub 2011 Oct 3. [21969609 ]

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