Multidrug resistance protein MexA

NameMultidrug resistance protein MexA
Synonyms
  • Multidrug resistance protein mexA precursor
Gene NamemexA
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Amino acid sequence
>lcl|BSEQ0011270|Multidrug resistance protein MexA
MQRTPAMRVLVPALLVAISALSGCGKSEAPPPAQTPEVGIVTLEAQTVTLNTELPGRTNA
FRIAEVRPQVNGIILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQEQAQRY
KLLVADQAVSKQQYADANAAYLQSKAAVEQARINLRYTKVLSPISGRIGRSAVTEGALVT
NGQANAMATVQQLDPIYVDVTQPSTALLRLRRELASGQLERAGDNAAKVSLKLEDGSQYP
LEGRLEFSEVSVDEGTGSVTIRAVFPNPNNELLPGMFVHAQLQEGVKQKAILAPQQGVTR
DLKGQATALVVNAQNKVELRVIKADRVIGDKWLVTEGLNAGDKIITEGLQFVQPGVEVKT
VPAKNVASAQKADAAPAKTDSKG
Number of residuesNone
Molecular Weight40969.34
Theoretical pI9.15
GO Classification
Functions
  • drug transmembrane transporter activity
Processes
  • response to antibiotic
  • drug transmembrane transport
Components
  • intrinsic component of plasma membrane
General FunctionDrug transmembrane transporter activity
Specific FunctionThe periplasmic linker component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Required for assembly of the MexA/MexB/OprM complex. Implicated in the secretion of the siderophore pyoverdine.The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDP52477
UniProtKB Entry Name
Cellular LocationCell inner membrane
Gene sequence
>lcl|BSEQ0011271|Multidrug resistance protein MexA (mexA)
ATGCAACGAACGCCAGCCATGCGTGTACTGGTTCCGGCCCTGCTGGTCGCGATTTCGGCC
CTTTCCGGGTGCGGAAAAAGCGAGGCGCCGCCGCCGGCGCAAACGCCGGAGGTCGGGATC
GTGACCCTGGAAGCGCAGACGGTGACCCTGAATACCGAGCTGCCGGGCCGGACCAATGCG
TTCCGCATCGCCGAGGTGCGTCCCCAGGTGAACGGCATCATCCTCAAGCGCCTGTTCAAG
GAAGGCAGCGACGTCAAGGCCGGGCAGCAGCTCTACCAGATCGACCCCGCCACCTACGAG
GCCGACTACCAGAGCGCCCAGGCCAACCTGGCTTCGACCCAGGAACAGGCCCAGCGCTAC
AAGCTGCTGGTCGCCGACCAGGCCGTGAGCAAGCAGCAGTACGCCGACGCCAATGCCGCC
TACCTGCAGTCCAAGGCGGCGGTGGAGCAGGCGCGGATCAACCTGCGCTACACCAAGGTG
CTGTCGCCGATCTCCGGCCGCATCGGCCGTTCCGCGGTGACCGAAGGCGCCCTGGTGACC
AACGGCCAGGCCAACGCGATGGCCACCGTGCAACAGCTCGACCCGATCTACGTCGACGTC
ACCCAGCCGTCCACCGCCCTGCTGCGCCTGCGCCGCGAACTGGCCAGCGGCCAGTTGGAG
CGCGCCGGCGACAACGCGGCGAAGGTCTCCCTGAAGCTGGAGGACGGTAGCCAATACCCG
CTGGAAGGTCGCCTCGAATTCTCCGAGGTTTCCGTCGACGAAGGCACCGGCTCGGTCACC
ATCCGCGCCGTGTTCCCCAACCCGAACAACGAGCTGCTGCCCGGCATGTTCGTTCACGCG
CAGTTGCAGGAAGGCGTCAAGCAGAAGGCCATCCTCGCTCCGCAGCAAGGCGTGACCCGC
GACCTCAAGGGCCAGGCTACCGCGCTGGTGGTGAACGCGCAGAACAAGGTCGAGCTGCGG
GTGATCAAGGCCGACCGGGTGATCGGCGACAAGTGGCTGGTTACCGAAGGCCTGAACGCC
GGCGACAAGATCATTACCGAAGGCCTGCAGTTCGTGCAGCCGGGTGTCGAGGTGAAGACC
GTGCCGGCGAAGAATGTCGCGTCCGCGCAGAAGGCCGACGCCGCTCCGGCGAAAACCGAC
AGCAAGGGCTGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC ID
Chromosome LocationNone
LocusNone
References
  1. Poole K, Heinrichs DE, Neshat S: Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine. Mol Microbiol. 1993 Nov;10(3):529-44. [7968531 ]
  2. Stover CK, Pham XQ, Erwin AL, Mizoguchi SD, Warrener P, Hickey MJ, Brinkman FS, Hufnagle WO, Kowalik DJ, Lagrou M, Garber RL, Goltry L, Tolentino E, Westbrock-Wadman S, Yuan Y, Brody LL, Coulter SN, Folger KR, Kas A, Larbig K, Lim R, Smith K, Spencer D, Wong GK, Wu Z, Paulsen IT, Reizer J, Saier MH, Hancock RE, Lory S, Olson MV: Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature. 2000 Aug 31;406(6799):959-64. [10984043 ]
  3. Blonder J, Goshe MB, Xiao W, Camp DG 2nd, Wingerd M, Davis RW, Smith RD: Global analysis of the membrane subproteome of Pseudomonas aeruginosa using liquid chromatography-tandem mass spectrometry. J Proteome Res. 2004 May-Jun;3(3):434-44. [15253424 ]
  4. Mahajan-Miklos S, Tan MW, Rahme LG, Ausubel FM: Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model. Cell. 1999 Jan 8;96(1):47-56. [9989496 ]
  5. Poole K, Krebes K, McNally C, Neshat S: Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon. J Bacteriol. 1993 Nov;175(22):7363-72. [8226684 ]
  6. Li XZ, Nikaido H, Poole K: Role of mexA-mexB-oprM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1995 Sep;39(9):1948-53. [8540696 ]
  7. Li XZ, Zhang L, Poole K: Role of the multidrug efflux systems of Pseudomonas aeruginosa in organic solvent tolerance. J Bacteriol. 1998 Jun;180(11):2987-91. [9603892 ]
  8. Yoneyama H, Maseda H, Kamiguchi H, Nakae T: Function of the membrane fusion protein, MexA, of the MexA, B-OprM efflux pump in Pseudomonas aeruginosa without an anchoring membrane. J Biol Chem. 2000 Feb 18;275(7):4628-34. [10671490 ]
  9. Mokhonov VV, Mokhonova EI, Akama H, Nakae T: Role of the membrane fusion protein in the assembly of resistance-nodulation-cell division multidrug efflux pump in Pseudomonas aeruginosa. Biochem Biophys Res Commun. 2004 Sep 17;322(2):483-9. [15325256 ]
  10. Akama H, Matsuura T, Kashiwagi S, Yoneyama H, Narita S, Tsukihara T, Nakagawa A, Nakae T: Crystal structure of the membrane fusion protein, MexA, of the multidrug transporter in Pseudomonas aeruginosa. J Biol Chem. 2004 Jun 18;279(25):25939-42. Epub 2004 Apr 26. [15117957 ]
  11. Higgins MK, Bokma E, Koronakis E, Hughes C, Koronakis V: Structure of the periplasmic component of a bacterial drug efflux pump. Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):9994-9. Epub 2004 Jun 28. [15226509 ]

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