Glycerol uptake facilitator protein

Name	Glycerol uptake facilitator protein
Synonyms	Aquaglyceroporin
Gene Name	glpF
Organism	Escherichia coli (strain K12)
Amino acid sequence	>lcl\|BSEQ0016653\|Glycerol uptake facilitator protein MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEISVIWGLGVAMAIYLTA GVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAALVYGLYYNLFFDFEQTHH IVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILMGLILALTDDGNGVPRGPLAP LLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAGWGNVAFTGGRDIPYFLVPLFGP IVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSEQKASL
Number of residues	None
Molecular Weight	29779.71
Theoretical pI	6.66
GO Classification	Functions metal ion binding glycerol channel activity water channel activity glycerol transmembrane transporter activity Processes ion transmembrane transport water transport cellular water homeostasis glycerol transport cellular response to mercury ion Components plasma membrane integral component of plasma membrane
General Function	Water channel activity
Specific Function	Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols.
Transmembrane Regions	6-34 40-60 85-108 144-169 178-194 232-254
GenBank Protein ID
UniProtKB ID	P0AER0
UniProtKB Entry Name
Cellular Location	Cell inner membrane
Gene sequence	>lcl\|BSEQ0016654\|Glycerol uptake facilitator protein (glpF) ATGAGTCAAACATCAACCTTGAAAGGCCAGTGCATTGCTGAATTCCTCGGTACCGGGTTG TTGATTTTCTTCGGTGTGGGTTGCGTTGCAGCACTAAAAGTCGCTGGTGCGTCTTTTGGT CAGTGGGAAATCAGTGTCATTTGGGGACTGGGGGTGGCAATGGCCATCTACCTGACCGCA GGGGTTTCCGGCGCGCATCTTAATCCCGCTGTTACCATTGCATTGTGGCTGTTTGCCTGT TTCGACAAGCGCAAAGTTATTCCTTTTATCGTTTCACAAGTTGCCGGCGCTTTCTGTGCT GCGGCTTTAGTTTACGGGCTTTACTACAATTTATTTTTCGACTTCGAGCAGACTCATCAC ATTGTTCGCGGCAGCGTTGAAAGTGTTGATCTGGCTGGCACTTTCTCTACTTACCCTAAT CCTCATATCAATTTTGTGCAGGCTTTCGCAGTTGAGATGGTGATTACCGCTATTCTGATG GGGCTGATCCTGGCGTTAACGGACGATGGCAACGGTGTACCACGCGGCCCTTTGGCTCCC TTGCTGATTGGTCTACTGATTGCGGTCATTGGCGCATCTATGGGCCCATTGACAGGTTTT GCCATGAACCCAGCGCGTGACTTCGGTCCGAAAGTCTTTGCCTGGCTGGCGGGCTGGGGC AATGTCGCCTTTACCGGCGGCAGAGACATTCCTTACTTCCTGGTGCCGCTTTTCGGCCCT ATCGTTGGCGCGATTGTAGGTGCATTTGCCTACCGCAAACTGATTGGTCGCCATTTGCCT TGCGATATCTGTGTTGTGGAAGAAAAGGAAACCACAACTCCTTCAGAACAAAAAGCTTCG CTGTAA
GenBank Gene ID
GeneCard ID	None
GenAtlas ID
HGNC ID
Chromosome Location	None
Locus	None
References	Muramatsu S, Mizuno T: Nucleotide sequence of the region encompassing the glpKF operon and its upstream region containing a bent DNA sequence of Escherichia coli. Nucleic Acids Res. 1989 Jun 12;17(11):4378. [2544860 ] Weissenborn DL, Wittekindt N, Larson TJ: Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12. J Biol Chem. 1992 Mar 25;267(9):6122-31. [1372899 ] Plunkett G 3rd, Burland V, Daniels DL, Blattner FR: Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res. 1993 Jul 25;21(15):3391-8. [8346018 ] Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y: The complete genome sequence of Escherichia coli K-12. Science. 1997 Sep 5;277(5331):1453-62. [9278503 ] Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T: Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Mol Syst Biol. 2006;2:2006.0007. Epub 2006 Feb 21. [16738553 ] Heller KB, Lin EC, Wilson TH: Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli. J Bacteriol. 1980 Oct;144(1):274-8. [6998951 ] Borgnia MJ, Agre P: Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2888-93. Epub 2001 Feb 20. [11226336 ] Daley DO, Rapp M, Granseth E, Melen K, Drew D, von Heijne G: Global topology analysis of the Escherichia coli inner membrane proteome. Science. 2005 May 27;308(5726):1321-3. [15919996 ] Fu D, Libson A, Miercke LJ, Weitzman C, Nollert P, Krucinski J, Stroud RM: Structure of a glycerol-conducting channel and the basis for its selectivity. Science. 2000 Oct 20;290(5491):481-6. [11039922 ] Unger VM: Fraternal twins: AQP1 and GlpF. Nat Struct Biol. 2000 Dec;7(12):1082-4. [11101882 ] Tajkhorshid E, Nollert P, Jensen MO, Miercke LJ, O'Connell J, Stroud RM, Schulten K: Control of the selectivity of the aquaporin water channel family by global orientational tuning. Science. 2002 Apr 19;296(5567):525-30. [11964478 ]

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