NADPH oxidase 4

NameNADPH oxidase 4
Synonyms
  • 1.6.3.-
  • Kidney oxidase-1
  • Kidney superoxide-producing NADPH oxidase
  • KOX-1
  • Renal NAD(P)H-oxidase
  • RENOX
Gene NameNOX4
OrganismHuman
Amino acid sequence
>lcl|BSEQ0009326|NADPH oxidase 4
MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYHYLHQMLGLGLCLSRAS
ASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSRTFHITCGVTICIFSGVHV
AAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVPGLTGVCMVVVLFLMITASTY
AIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTNLDTHPPGCISLNRTSSQNISLP
EYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRFQANFPQTWLWISGPLCLYCAERLY
RYIRSNKPVTIISVMSHPSDVMEIRMVKENFKARPGQYITLHCPSVSALENHPFTLTMCP
TETKATFGVHLKIVGDWTERFRDLLLPPSSQDSEILPFIQSRNYPKLYIDGPFGSPFEES
LNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLRRLYFIWVCRDIQSFRWFADLLCMLH
NKFWQENRPDYVNIQLYLSQTDGIQKIIGEKYHALNSRLFIGRPRWKLLFDEIAKYNRGK
TVGVFCCGPNSLSKTLHKLSNQNNSYGTRFEYNKESFS
Number of residues578
Molecular Weight66930.995
Theoretical pINone
GO Classification
Functions
  • oxygen sensor activity
  • nucleotide binding
  • heme binding
  • modified amino acid binding
  • superoxide-generating NADPH oxidase activity
  • electron carrier activity
  • NAD(P)H oxidase activity
  • flavin adenine dinucleotide binding
Processes
  • reactive oxygen species metabolic process
  • positive regulation of MAP kinase activity
  • bone resorption
  • cell aging
  • positive regulation of stress fiber assembly
  • positive regulation of DNA biosynthetic process
  • cardiac muscle cell differentiation
  • positive regulation of apoptotic process
  • positive regulation of ERK1 and ERK2 cascade
  • superoxide metabolic process
  • cellular response to transforming growth factor beta stimulus
  • positive regulation of smooth muscle cell migration
  • cellular response to gamma radiation
  • oxidation-reduction process
  • negative regulation of cell proliferation
  • homocysteine metabolic process
  • inflammatory response
  • response to hypoxia
  • cellular response to cAMP
  • superoxide anion generation
  • positive regulation of reactive oxygen species metabolic process
  • positive regulation of protein kinase B signaling
  • cellular response to glucose stimulus
  • gene expression
  • cell morphogenesis
Components
  • nucleolus
  • focal adhesion
  • perinuclear region of cytoplasm
  • mitochondrion
  • endoplasmic reticulum membrane
  • NADPH oxidase complex
  • integral component of membrane
  • stress fiber
  • apical plasma membrane
General FunctionSuperoxide-generating nadph oxidase activity
Specific FunctionConstitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity.Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1.
Transmembrane Regions17-37 63-83 104-124 155-175 189-209 425-445
GenBank Protein ID
UniProtKB IDQ9NPH5
UniProtKB Entry NameNOX4_HUMAN
Cellular LocationEndoplasmic reticulum membrane
Gene sequence
>lcl|BSEQ0013803|NADPH oxidase 4 (NOX4)
ATGGCTGTGTCCTGGAGGAGCTGGCTCGCCAACGAAGGGGTTAAACACCTCTGCCTGTTC
ATCTGGCTCTCCATGAATGTCCTGCTTTTCTGGAAAACCTTCTTGCTGTATAACCAAGGG
CCAGAGTATCACTACCTCCACCAGATGTTGGGGCTAGGATTGTGTCTAAGCAGAGCCTCA
GCATCTGTTCTTAACCTCAACTGCAGCCTTATCCTTTTACCCATGTGCCGAACACTCTTG
GCTTACCTCCGAGGATCACAGAAGGTTCCAAGCAGGAGAACCAGGAGATTGTTGGATAAA
AGCAGAACATTCCATATTACCTGTGGTGTTACTATCTGTATTTTCTCAGGCGTGCATGTG
GCTGCCCATCTGGTGAATGCCCTCAACTTCTCAGTGAATTACAGTGAAGACTTTGTTGAA
CTGAATGCAGCAAGATACCGAGATGAGGATCCTAGAAAACTTCTCTTCACAACTGTTCCT
GGCCTGACAGGGGTCTGCATGGTGGTGGTGCTATTCCTCATGATCACAGCCTCTACATAT
GCAATAAGAGTTTCTAACTATGATATCTTCTGGTATACTCATAACCTCTTCTTTGTCTTC
TACATGCTGCTGACGTTGCATGTTTCAGGAGGGCTGCTGAAGTATCAAACTAATTTAGAT
ACCCACCCTCCCGGCTGCATCAGTCTTAACCGAACCAGCTCTCAGAATATTTCCTTACCA
GAGTATTTCTCAGAACATTTTCATGAACCTTTCCCTGAAGGATTTTCAAAACCGGCAGAG
TTTACCCAGCACAAATTTGTGAAGATTTGTATGGAAGAGCCCAGATTCCAAGCTAATTTT
CCACAGACTTGGCTTTGGATTTCTGGACCTTTGTGCCTGTACTGTGCCGAAAGACTTTAC
AGGTATATCCGGAGCAATAAGCCAGTCACCATCATTTCGGTCATAAGTCATCCCTCAGAT
GTCATGGAAATCCGAATGGTCAAAGAAAATTTTAAAGCAAGACCTGGTCAGTATATTACT
CTACATTGTCCCAGTGTATCTGCATTAGAAAATCATCCATTTACCCTCACAATGTGTCCA
ACTGAAACCAAAGCAACATTTGGGGTTCATCTTAAAATAGTAGGAGACTGGACAGAACGA
TTTCGAGATTTACTACTGCCTCCATCTAGTCAAGACTCCGAAATTCTGCCCTTCATTCAA
TCTAGAAATTATCCCAAGGATGACTGGAAACCATACAAGCTTAGAAGACTATACTTTATT
TGGGTATGCAGAGATATCCAGTCCTTCCGTTGGTTTGCAGATTTACTCTGTATGTTGCAT
AACAAGTTTTGGCAAGAGAACAGACCTGACTATGTCAACATCCAGCTGTACCTCAGTCAA
ACAGATGGGATACAGAAGATAATTGGAGAAAAATATCATGCACTGAATTCAAGACTGTTT
ATAGGACGTCCTCGGTGGAAACTTTTGTTTGATGAAATAGCAAAATATAACAGAGGAAAA
ACAGTTGGTGTTTTCTGTTGTGGACCCAATTCACTATCCAAGACTCTTCATAAACTGAGT
AACCAGAACAACTCATATGGGACAAGATTTGAATACAATAAAGAGTCTTTCAGCTGA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC IDHGNC:7891
Chromosome Location11
LocusNone
References
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  2. Cheng G, Cao Z, Xu X, van Meir EG, Lambeth JD: Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4, and Nox5. Gene. 2001 May 16;269(1-2):131-40.[11376945 ]
  3. Shiose A, Kuroda J, Tsuruya K, Hirai M, Hirakata H, Naito S, Hattori M, Sakaki Y, Sumimoto H: A novel superoxide-producing NAD(P)H oxidase in kidney. J Biol Chem. 2001 Jan 12;276(2):1417-23.[11032835 ]
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