Lysine-specific demethylase PHF2

NameLysine-specific demethylase PHF2
Synonyms
  • 1.14.11.-
  • CENP-35
  • GRC5
  • KIAA0662
  • PHD finger protein 2
Gene NamePHF2
OrganismHuman
Amino acid sequence
>lcl|BSEQ0037291|Lysine-specific demethylase PHF2
MATVPVYCVCRLPYDVTRFMIECDACKDWFHGSCVGVEEEEAPDIDIYHCPNCEKTHGKS
TLKKKRTWHKHGPGQAPDVKPVQNGSQLFIKELRSRTFPSAEDVVARVPGSQLTLGYMEE
HGFTEPILVPKKDGLGLAVPAPTFYVSDVENYVGPERSVDVTDVTKQKDCKMKLKEFVDY
YYSTNRKRVLNVTNLEFSDTRMSSFVEPPDIVKKLSWVENYWPDDALLAKPKVTKYCLIC
VKDSYTDFHIDSGGASAWYHVLKGEKTFYLIRPASANISLYERWRSASNHSEMFFADQVD
KCYKCIVKQGQTLFIPSGWIYATLTPVDCLAFAGHFLHSLSVEMQMRAYEVERRLKLGSL
TQFPNFETACWYMGKHLLEAFKGSHKSGKQLPPHLVQGAKILNGAFRSWTKKQALAEHED
ELPEHFKPSQLIKDLAKEIRLSENASKAVRPEVNTVASSDEVCDGDREKEEPPSPIEATP
PQSLLEKVSKKKTPKTVKMPKPSKIPKPPKPPKPPRPPKTLKLKDGGKKKGKKSRESASP
TIPNLDLLEAHTKEALTKMEPPKKGKATKSVLSVPNKDVVHMQNDVERLEIREQTKSKSE
AKWKYKNSKPDSLLKMEEEQKLEKSPLAGNKDNKFSFSFSNKKLLGSKALRPPTSPGVFG
ALQNFKEDKPKPVRDEYEYVSDDGELKIDEFPIRRKKNAPKRDLSFLLDKKAVLPTPVTK
PKLDSAAYKSDDSSDEGSLHIDTDTKPGRNARVKKESGSSAAGILDLLQASEEVGALEYN
PSSQPPASPSTQEAIQGMLSMANLQASDSCLQTTWGAGQAKGSSLAAHGARKNGGGSGKS
AGKRLLKRAAKNSVDLDDYEEEQDHLDACFKDSDYVYPSLESDEDNPIFKSRSKKRKGSD
DAPYSPTARVGPSVPRQDRPVREGTRVASIETGLAAAAAKLSQQEEQKSKKKKSAKRKLT
PNTTSPSTSTSISAGTTSTSTTPASTTPASTTPASTSTASSQASQEGSSPEPPPESHSSS
LADHEYTAAGTFTGAQAGRTSQPMAPGVFLTQRRPSASSPNNNTAAKGKRTKKGMATAKQ
RLGKILKIHRNGKLLL
Number of residues1096
Molecular Weight120773.925
Theoretical pINone
GO Classification
Functions
  • dioxygenase activity
  • iron ion binding
  • methylated histone binding
  • zinc ion binding
  • histone demethylase activity (H3-K9 specific)
Processes
  • chromatin organization
  • protein demethylation
  • negative regulation of chromatin silencing at rDNA
  • liver development
  • transcription, DNA-templated
Components
  • condensed chromosome kinetochore
  • cytoplasm
  • nucleus
  • nucleolus
  • nucleoplasm
General FunctionZinc ion binding
Specific FunctionLysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.
Transmembrane Regions
GenBank Protein ID
UniProtKB IDO75151
UniProtKB Entry NamePHF2_HUMAN
Cellular LocationNucleus
Gene sequence
>lcl|BSEQ0017853|Lysine-specific demethylase PHF2 (PHF2)
ATGGCGACGGTGCCCGTGTACTGCGTCTGCCGGCTCCCCTACGACGTTACCCGCTTCATG
ATCGAGTGCGACGCCTGCAAGGACTGGTTCCACGGCAGCTGTGTTGGGGTGGAAGAGGAG
GAGGCGCCCGACATCGACATATACCACTGCCCAAACTGTGAGAAAACCCATGGGAAGTCC
ACCTTAAAGAAGAAGCGGACCTGGCACAAACACGGCCCGGGGCAAGCGCCTGACGTCAAG
CCCGTGCAGAATGGCAGCCAGCTCTTCATCAAGGAGCTGCGGAGCCGGACCTTTCCCAGT
GCTGAAGACGTGGTGGCCCGTGTGCCAGGAAGTCAGCTCACGCTGGGCTACATGGAGGAG
CACGGCTTCACCGAGCCCATCCTCGTCCCTAAGAAAGACGGGCTGGGTCTGGCTGTCCCG
GCCCCCACGTTCTATGTCAGTGACGTCGAGAACTACGTGGGGCCGGAACGGAGTGTGGAT
GTGACAGATGTCACCAAGCAGAAGGACTGCAAGATGAAGCTGAAGGAGTTTGTGGACTAT
TACTACAGCACCAACCGCAAGCGGGTCCTCAACGTCACCAACCTCGAGTTCTCTGACACC
CGAATGTCCAGCTTCGTGGAGCCACCTGACATTGTAAAGAAACTGTCATGGGTAGAAAAC
TACTGGCCAGATGATGCATTGCTGGCCAAGCCCAAAGTGACCAAGTACTGCCTAATCTGC
GTGAAGGACAGTTACACCGACTTCCACATCGACTCTGGGGGCGCCTCTGCCTGGTACCAC
GTGCTCAAGGGGGAGAAGACCTTCTATCTCATCAGGCCGGCCTCGGCCAACATCTCCCTG
TATGAGCGCTGGCGGTCTGCCTCTAACCACAGCGAGATGTTCTTTGCTGACCAGGTCGAC
AAATGCTACAAGTGCATCGTCAAGCAGGGCCAGACCCTCTTCATCCCCTCAGGCTGGATC
TACGCCACACTCACCCCTGTGGACTGCCTGGCCTTCGCGGGACATTTCCTCCACAGCCTG
AGTGTGGAGATGCAGATGAGAGCATATGAAGTGGAAAGGAGGTTGAAACTGGGCAGCCTG
ACTCAGTTTCCCAACTTTGAAACTGCGTGCTGGTACATGGGGAAGCACCTGCTGGAGGCA
TTCAAAGGCTCTCACAAATCTGGGAAGCAGCTGCCCCCTCATCTAGTCCAAGGAGCTAAA
ATTCTCAATGGTGCTTTCCGATCGTGGACGAAGAAGCAGGCTTTGGCAGAGCATGAGGAC
GAGCTCCCGGAGCACTTCAAACCTTCACAGCTAATCAAAGACCTGGCCAAAGAGATCCGG
CTCAGTGAGAATGCCTCCAAAGCCGTCCGACCGGAAGTGAATACTGTCGCCTCGTCAGAT
GAGGTGTGTGACGGGGACCGGGAGAAGGAGGAGCCCCCGTCTCCCATTGAGGCCACCCCG
CCTCAATCCCTCCTGGAGAAAGTGTCCAAAAAAAAGACTCCCAAAACTGTGAAGATGCCC
AAGCCATCCAAAATCCCCAAGCCCCCGAAGCCCCCTAAGCCCCCAAGGCCCCCCAAAACG
CTGAAGCTCAAAGATGGAGGCAAGAAGAAAGGGAAGAAGTCCCGGGAGTCAGCCTCACCC
ACCATCCCCAACCTGGACCTGCTCGAAGCCCACACCAAGGAGGCACTGACCAAGATGGAG
CCGCCCAAGAAGGGCAAGGCCACAAAGAGTGTCCTGAGTGTGCCCAACAAAGATGTGGTT
CACATGCAGAATGATGTGGAGAGGCTGGAAATTCGAGAGCAAACCAAGAGCAAGTCAGAG
GCCAAGTGGAAGTACAAGAACAGCAAACCTGACTCCTTACTGAAGATGGAAGAGGAGCAG
AAGCTAGAGAAGTCGCCTCTAGCTGGAAACAAAGACAATAAGTTCTCTTTTTCTTTCTCC
AACAAGAAACTCCTCGGCTCCAAGGCTCTCAGGCCCCCGACGAGCCCTGGTGTGTTCGGG
GCCTTGCAGAACTTCAAGGAGGACAAGCCCAAGCCCGTGCGGGATGAGTATGAGTACGTG
TCGGATGACGGTGAGCTCAAGATCGACGAGTTTCCCATCAGGAGGAAGAAAAACGCCCCG
AAAAGGGACTTGTCCTTCTTGTTGGATAAGAAGGCTGTGCTGCCCACGCCTGTCACGAAG
CCAAAGCTGGACTCGGCAGCGTACAAGAGTGATGACTCCTCGGACGAGGGTTCGCTGCAC
ATCGACACAGACACCAAGCCCGGCCGCAATGCCAGAGTCAAGAAGGAGAGTGGGAGCTCG
GCAGCTGGCATCTTGGACCTGCTGCAGGCCAGTGAGGAGGTTGGCGCGCTGGAGTACAAC
CCCAGCAGCCAGCCCCCGGCCTCCCCCAGCACACAGGAAGCCATTCAGGGAATGCTGTCC
ATGGCCAACCTGCAGGCCTCCGACTCCTGCCTGCAGACCACGTGGGGAGCTGGCCAGGCC
AAGGGGAGCTCGCTGGCTGCCCATGGTGCCCGGAAGAATGGGGGTGGCAGTGGCAAGAGT
GCAGGCAAACGACTGCTGAAGAGGGCTGCCAAGAACAGTGTCGACCTGGACGACTACGAG
GAAGAGCAGGACCACCTGGATGCCTGCTTCAAGGACTCAGACTACGTTTACCCCTCACTG
GAGTCAGATGAAGACAACCCCATCTTTAAGTCCCGGTCGAAGAAAAGGAAAGGCTCAGAC
GACGCTCCCTACAGCCCAACAGCAAGGGTCGGCCCATCGGTGCCAAGACAGGACAGGCCT
GTGCGTGAGGGTACACGGGTGGCTTCCATCGAGACCGGGCTGGCGGCTGCTGCAGCTAAG
TTGTCCCAGCAGGAGGAGCAGAAAAGCAAGAAAAAAAAGAGTGCCAAGAGGAAGCTGACT
CCTAACACCACCTCCCCTTCCACCTCCACCTCCATCTCTGCCGGCACCACCTCCACCTCC
ACCACGCCAGCCTCTACCACCCCGGCCTCCACCACCCCGGCCTCCACCAGCACGGCCAGC
AGCCAGGCCTCGCAGGAGGGCAGCTCGCCAGAGCCCCCGCCTGAGTCGCATAGCAGCAGC
CTGGCGGACCATGAGTACACAGCCGCTGGCACCTTCACCGGGGCCCAGGCTGGCCGCACC
TCCCAGCCCATGGCCCCTGGGGTCTTTCTCACACAGAGGCGGCCCTCCGCATCGTCTCCA
AACAACAACACCGCTGCCAAAGGAAAACGTACGAAAAAGGGCATGGCGACCGCCAAGCAG
AGGCTTGGGAAAATTTTGAAAATTCATCGGAACGGGAAACTACTCCTTTAA
GenBank Gene ID
GeneCard IDNone
GenAtlas ID
HGNC IDHGNC:8920
Chromosome Location9
LocusNone
References
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  3. Nakajima D, Okazaki N, Yamakawa H, Kikuno R, Ohara O, Nagase T: Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. DNA Res. 2002 Jun 30;9(3):99-106.[12168954 ]
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