Enzyme

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     1. Oxidoreductases
        1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)
            1.13.11 With incorporation of two atoms of oxygen
ID:1.13.11.51
Description:9-cis-epoxycarotenoid dioxygenase.
Alternative Name: VP14.
Nine-cis-epoxycarotenoid dioxygenase.
NCED.

3D structure

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References

External Links

UniProtKB Enzyme Link: UniProtKB 1.13.11.51
BRENDA Enzyme Link: BRENDA 1.13.11.51
KEGG Enzyme Link: KEGG1.13.11.51
BioCyc Enzyme Link: BioCyc 1.13.11.51
ExPASy Enzyme Link: ExPASy1.13.11.51
EC2PDB Enzyme Link: EC2PDB 1.13.11.51
ExplorEnz Enzyme Link: ExplorEnz 1.13.11.51
PRIAM enzyme-specific profiles Link: PRIAM 1.13.11.51
IntEnz Enzyme Link: IntEnz 1.13.11.51
MEDLINE Enzyme Link: MEDLINE 1.13.11.51
MSA:

1.13.11.51;

Phylogenetic Tree:

1.13.11.51;

Uniprot:
M-CSA:
RHEA:16541 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin
RULE(radius=1) [*:1]-[CH;+0:2]=[CH;+0:3]-[*:4].[O;H0;+0:5]=[O;H0;+0:6]>>[*:1]-[CH;+0:2]=[O;H0;+0:5].[*:4]-[CH;+0:3]=[O;H0;+0:6]
Reaction
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Genetic control of abscisic acid biosynthesis in maize.Tan BC, Schwartz SH, Zeevaart JA, McCarty DR1997 Oct 289342392
Specific oxidative cleavage of carotenoids by VP14 of maize.Schwartz SH, Tan BC, Gage DA, Zeevaart JA, McCarty DR1997 Jun 209188535
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K2001 Aug11532178
Ectopic expression of a tomato 9-cis-epoxycarotenoid dioxygenase gene causes over-production of abscisic acid.Thompson AJ, Jackson AC, Symonds RC, Mulholland BJ, Dadswell AR, Blake PS, Burbidge A, Taylor IB2000 Aug10929129
The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean.Qin X, Zeevaart JA1999 Dec 2110611388

RHEA:19677 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al + 2-cis,4-trans-xanthoxin
RULE(radius=1) [*:1]-[CH;+0:2]=[CH;+0:3]-[*:4].[O;H0;+0:5]=[O;H0;+0:6]>>[*:1]-[CH;+0:2]=[O;H0;+0:5].[*:4]-[CH;+0:3]=[O;H0;+0:6]
Reaction
Core-to-Core More
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Genetic control of abscisic acid biosynthesis in maize.Tan BC, Schwartz SH, Zeevaart JA, McCarty DR1997 Oct 289342392
Specific oxidative cleavage of carotenoids by VP14 of maize.Schwartz SH, Tan BC, Gage DA, Zeevaart JA, McCarty DR1997 Jun 209188535
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K2001 Aug11532178
Ectopic expression of a tomato 9-cis-epoxycarotenoid dioxygenase gene causes over-production of abscisic acid.Thompson AJ, Jackson AC, Symonds RC, Mulholland BJ, Dadswell AR, Blake PS, Burbidge A, Taylor IB2000 Aug10929129
The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean.Qin X, Zeevaart JA1999 Dec 2110611388

RHEA:23328 a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal
RULE(radius=1) [*:1]-[CH;+0:2]=[CH;+0:3]-[*:4].[O;H0;+0:5]=[O;H0;+0:6]>>[*:4]-[CH;+0:3]=[O;H0;+0:5].[*:1]-[CH;+0:2]=[O;H0;+0:6]
Reaction
Core-to-Core No scaffolds atoms were exchanged as a result of the reaction

References

TitleAuthorsDatePubMed ID
Genetic control of abscisic acid biosynthesis in maize.Tan BC, Schwartz SH, Zeevaart JA, McCarty DR1997 Oct 289342392
Specific oxidative cleavage of carotenoids by VP14 of maize.Schwartz SH, Tan BC, Gage DA, Zeevaart JA, McCarty DR1997 Jun 209188535
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K2001 Aug11532178
Ectopic expression of a tomato 9-cis-epoxycarotenoid dioxygenase gene causes over-production of abscisic acid.Thompson AJ, Jackson AC, Symonds RC, Mulholland BJ, Dadswell AR, Blake PS, Burbidge A, Taylor IB2000 Aug10929129
The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean.Qin X, Zeevaart JA1999 Dec 2110611388