EC number:1.14.13.178
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.13.178 |
|---|---|
| Description: | Methylxanthine N(1)-demethylase. |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.13.178 |
| BRENDA Enzyme Link: | BRENDA 1.14.13.178 |
| KEGG Enzyme Link: | KEGG1.14.13.178 |
| BioCyc Enzyme Link: | BioCyc 1.14.13.178 |
| ExPASy Enzyme Link: | ExPASy1.14.13.178 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.13.178 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.13.178 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.13.178 |
| IntEnz Enzyme Link: | IntEnz 1.14.13.178 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.13.178 |
EC number:1.14.13.178
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:30315 | 1,7-dimethylxanthine + H(+) + NADH + O2 = 7-methylxanthine + formaldehyde + H2O + NAD(+) |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[n;H0;+0:10](:[*:11])-[CH3;+0:12].[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[nH;+0:10]:[*:11].[CH2;+0:12]=[O;H0;+0:14].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids. | Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M | 2012 Apr | 22328667 |
| Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. | Summers RM, Louie TM, Yu CL, Subramanian M | 2011 Feb | 20966097 |
| RHEA:36267 | caffeine + H(+) + NADH + O2 = formaldehyde + H2O + NAD(+) + theobromine |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[n;H0;+0:10](:[*:11])-[CH3;+0:12].[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[nH;+0:10]:[*:11].[CH2;+0:12]=[O;H0;+0:14].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Enzymological aspects of caffeine demethylation and formaldehyde oxidation by Pseudomonas putida C1. | Hohnloser W, Osswald B, Lingens F | 1980 Dec | 7461603 |
| Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids. | Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M | 2012 Apr | 22328667 |
| Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. | Summers RM, Louie TM, Yu CL, Subramanian M | 2011 Feb | 20966097 |
| RHEA:36271 | caffeine + H(+) + NADPH + O2 = formaldehyde + H2O + NADP(+) + theobromine |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[n;H0;+0:10](:[*:11])-[CH3;+0:12].[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[nH;+0:10]:[*:11].[CH2;+0:12]=[O;H0;+0:14].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Enzymological aspects of caffeine demethylation and formaldehyde oxidation by Pseudomonas putida C1. | Hohnloser W, Osswald B, Lingens F | 1980 Dec | 7461603 |
| Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids. | Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M | 2012 Apr | 22328667 |
| Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. | Summers RM, Louie TM, Yu CL, Subramanian M | 2011 Feb | 20966097 |
| RHEA:36275 | H(+) + NADPH + O2 + theophylline = 3-methylxanthine + formaldehyde + H2O + NADP(+) |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[n;H0;+0:10](:[*:11])-[CH3;+0:12].[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[nH;+0:10]:[*:11].[CH2;+0:12]=[O;H0;+0:14].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids. | Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M | 2012 Apr | 22328667 |
| Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. | Summers RM, Louie TM, Yu CL, Subramanian M | 2011 Feb | 20966097 |
| RHEA:36279 | H(+) + NADH + O2 + theophylline = 3-methylxanthine + formaldehyde + H2O + NAD(+) |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[n;H0;+0:10](:[*:11])-[CH3;+0:12].[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[nH;+0:10]:[*:11].[CH2;+0:12]=[O;H0;+0:14].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids. | Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M | 2012 Apr | 22328667 |
| Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. | Summers RM, Louie TM, Yu CL, Subramanian M | 2011 Feb | 20966097 |
| RHEA:36283 | 1,7-dimethylxanthine + H(+) + NADPH + O2 = 7-methylxanthine + formaldehyde + H2O + NADP(+) |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[n;H0;+0:10](:[*:11])-[CH3;+0:12].[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[nH;+0:10]:[*:11].[CH2;+0:12]=[O;H0;+0:14].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Novel, highly specific N-demethylases enable bacteria to live on caffeine and related purine alkaloids. | Summers RM, Louie TM, Yu CL, Gakhar L, Louie KC, Subramanian M | 2012 Apr | 22328667 |
| Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source. | Summers RM, Louie TM, Yu CL, Subramanian M | 2011 Feb | 20966097 |