EC number:1.14.13.181
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.13.181 |
|---|---|
| Description: | 13-deoxydaunorubicin hydroxylase. |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.13.181 |
| BRENDA Enzyme Link: | BRENDA 1.14.13.181 |
| KEGG Enzyme Link: | KEGG1.14.13.181 |
| BioCyc Enzyme Link: | BioCyc 1.14.13.181 |
| ExPASy Enzyme Link: | ExPASy1.14.13.181 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.13.181 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.13.181 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.13.181 |
| IntEnz Enzyme Link: | IntEnz 1.14.13.181 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.13.181 |
EC number:1.14.13.181
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:15717 | daunorubicin + H(+) + NADPH + O2 = doxorubicin + H2O + NADP(+) |
| RULE(radius=1) | [*:1]-[CH3;+0:2].[*:3]-[N;H0;+0:4]1-[CH;+0:5]=[C;H0;+0:6](-[*:7])-[CH2;+0:8]-[CH;+0:9]=[CH;+0:10]-1.[H+;H0:11].[O;H0;+0:12]=[O;H0;+0:13]>>[*:1]-[CH2;+0:2]-[OH;+0:12].[*:3]-[n+;H0:4]1:[cH;+0:5]:[c;H0;+0:6](-[*:7]):[cH;+0:8]:[cH;+0:9]:[cH;+0:10]:1.[OH2;+0:13] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification, properties, and characterization of recombinant Streptomyces sp. strain C5 DoxA, a cytochrome P-450 catalyzing multiple steps in doxorubicin biosynthesis. | Walczak RJ, Dickens ML, Priestley ND, Strohl WR | 1999 Jan | 9864343 |
| In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA. | Dickens ML, Priestley ND, Strohl WR | 1997 Apr | 9098063 |
| Adriamycin, 14-hydroxydaunomycin, a new antitumor antibiotic from S. peucetius var. caesius. | Arcamone F, Cassinelli G, Fantini G, Grein A, Orezzi P, Pol C, Spalla C | 1969 Nov | 5365804 |
| RHEA:37847 | 13-dihydrodaunorubicin + H(+) + NADPH + O2 = daunorubicin + 2 H2O + NADP(+) |
| RULE(radius=1) | [*:1]-[CH;+0:2](-[*:3])-[OH;+0:4].[*:5]-[N;H0;+0:6]1-[CH;+0:7]=[C;H0;+0:8](-[*:9])-[CH2;+0:10]-[CH;+0:11]=[CH;+0:12]-1.[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[C;H0;+0:2](-[*:3])=[O;H0;+0:4].[*:5]-[n+;H0:6]1:[cH;+0:7]:[c;H0;+0:8](-[*:9]):[cH;+0:10]:[cH;+0:11]:[cH;+0:12]:1.[OH2;+0:14].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification, properties, and characterization of recombinant Streptomyces sp. strain C5 DoxA, a cytochrome P-450 catalyzing multiple steps in doxorubicin biosynthesis. | Walczak RJ, Dickens ML, Priestley ND, Strohl WR | 1999 Jan | 9864343 |
| In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA. | Dickens ML, Priestley ND, Strohl WR | 1997 Apr | 9098063 |
| Bioconversion of the anthracycline analogue desacetyladriamycin by recombinant DoxA, a P450-monooxygenase from Streptomyces sp. strain C5. | Walczak RJ, Hines JV, Strohl WR, Priestley ND | 2001 Jul 26 | 11463295 |
| RHEA:37851 | 13-deoxydaunorubicin + H(+) + NADPH + O2 = 13-dihydrodaunorubicin + H2O + NADP(+) |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[*:3].[*:4]-[N;H0;+0:5]1-[CH;+0:6]=[C;H0;+0:7](-[*:8])-[CH2;+0:9]-[CH;+0:10]=[CH;+0:11]-1.[H+;H0:12].[O;H0;+0:13]=[O;H0;+0:14]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:13].[*:4]-[n+;H0:5]1:[cH;+0:6]:[c;H0;+0:7](-[*:8]):[cH;+0:9]:[cH;+0:10]:[cH;+0:11]:1.[OH2;+0:14] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification, properties, and characterization of recombinant Streptomyces sp. strain C5 DoxA, a cytochrome P-450 catalyzing multiple steps in doxorubicin biosynthesis. | Walczak RJ, Dickens ML, Priestley ND, Strohl WR | 1999 Jan | 9864343 |
| In vivo and in vitro bioconversion of epsilon-rhodomycinone glycoside to doxorubicin: functions of DauP, DauK, and DoxA. | Dickens ML, Priestley ND, Strohl WR | 1997 Apr | 9098063 |
| Bioconversion of the anthracycline analogue desacetyladriamycin by recombinant DoxA, a P450-monooxygenase from Streptomyces sp. strain C5. | Walczak RJ, Hines JV, Strohl WR, Priestley ND | 2001 Jul 26 | 11463295 |