EC number:1.14.13.200
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.13.200 |
|---|---|
| Description: | Tetracenomycin A2 monooxygenase-diooxygenase. |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.13.200 |
| BRENDA Enzyme Link: | BRENDA 1.14.13.200 |
| KEGG Enzyme Link: | KEGG1.14.13.200 |
| BioCyc Enzyme Link: | BioCyc 1.14.13.200 |
| ExPASy Enzyme Link: | ExPASy1.14.13.200 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.13.200 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.13.200 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.13.200 |
| IntEnz Enzyme Link: | IntEnz 1.14.13.200 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.13.200 |
EC number:1.14.13.200
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:42824 | 2 H(+) + 2 NADH + 2 O2 + tetracenomycin A2 = H2O + 2 NAD(+) + tetracenomycin C |
| RULE(radius=1) | [*:1]-[C;H0;+0:2]1=[CH;+0:3]-[N;H0;+0:4](-[*:5])-[CH;+0:6]=[CH;+0:7]-[CH2;+0:8]-1.[*:9]-[N;H0;+0:10]1-[CH;+0:11]=[C;H0;+0:12](-[*:13])-[CH2;+0:14]-[CH;+0:15]=[CH;+0:16]-1.[*:17]-[c;H0;+0:18]1:[cH;+0:19]:[c;H0;+0:20](-[*:21]):[cH;+0:22]:[c;H0;+0:23](-[OH;+0:24]):[c;H0;+0:25]:1-[*:26].[H+;H0:27].[H+;H0:28].[O;H0;+0:29]=[O;H0;+0:30].[O;H0;+0:31]=[O;H0;+0:32]>>[*:17]-[C;H0;+0:18]1(-[OH;+0:30])-[CH;+0:19](-[OH;+0:31])-[C;H0;+0:20](-[*:21])=[CH;+0:22]-[C;H0;+0:23](=[O;H0;+0:24])-[C;H0;+0:25]-1(-[*:26])-[OH;+0:32].[*:1]-[c;H0;+0:2]1:[cH;+0:3]:[n+;H0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]-[n+;H0:10]1:[cH;+0:11]:[c;H0;+0:12](-[*:13]):[cH;+0:14]:[cH;+0:15]:[cH;+0:16]:1.[OH2;+0:29] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Triple hydroxylation of tetracenomycin A2 to tetracenomycin C in Streptomyces glaucescens. Overexpression of the tcmG gene in Streptomyces lividans and characterization of the tetracenomycin A2 oxygenase. | Shen B, Hutchinson CR | 1994 Dec 2 | 7982994 |
| Crystallization and preliminary X-ray analysis of tetracenomycin A2 oxygenase: a flavoprotein hydroxylase involved in polyketide biosynthesis. | Beynon J, Rafanan ER Jr, Shen B, Fisher AJ | 2000 Dec | 11092935 |
| Triple hydroxylation of tetracenomycin A2 to tetracenomycin C involving two molecules of O(2) and one molecule of H(2)O. | Rafanan ER Jr, Hutchinson CR, Shen B | 2000 Oct 5 | 11009387 |
| RHEA:42820 | 2 H(+) + 2 NADPH + 2 O2 + tetracenomycin A2 = H2O + 2 NADP(+) + tetracenomycin C |
| RULE(radius=1) | [*:1]-[C;H0;+0:2]1=[CH;+0:3]-[N;H0;+0:4](-[*:5])-[CH;+0:6]=[CH;+0:7]-[CH2;+0:8]-1.[*:9]-[N;H0;+0:10]1-[CH;+0:11]=[C;H0;+0:12](-[*:13])-[CH2;+0:14]-[CH;+0:15]=[CH;+0:16]-1.[*:17]-[c;H0;+0:18]1:[cH;+0:19]:[c;H0;+0:20](-[*:21]):[cH;+0:22]:[c;H0;+0:23](-[OH;+0:24]):[c;H0;+0:25]:1-[*:26].[H+;H0:27].[H+;H0:28].[O;H0;+0:29]=[O;H0;+0:30].[O;H0;+0:31]=[O;H0;+0:32]>>[*:17]-[C;H0;+0:18]1(-[OH;+0:30])-[CH;+0:19](-[OH;+0:31])-[C;H0;+0:20](-[*:21])=[CH;+0:22]-[C;H0;+0:23](=[O;H0;+0:24])-[C;H0;+0:25]-1(-[*:26])-[OH;+0:32].[*:1]-[c;H0;+0:2]1:[cH;+0:3]:[n+;H0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]-[n+;H0:10]1:[cH;+0:11]:[c;H0;+0:12](-[*:13]):[cH;+0:14]:[cH;+0:15]:[cH;+0:16]:1.[OH2;+0:29] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Triple hydroxylation of tetracenomycin A2 to tetracenomycin C in Streptomyces glaucescens. Overexpression of the tcmG gene in Streptomyces lividans and characterization of the tetracenomycin A2 oxygenase. | Shen B, Hutchinson CR | 1994 Dec 2 | 7982994 |
| Crystallization and preliminary X-ray analysis of tetracenomycin A2 oxygenase: a flavoprotein hydroxylase involved in polyketide biosynthesis. | Beynon J, Rafanan ER Jr, Shen B, Fisher AJ | 2000 Dec | 11092935 |
| Triple hydroxylation of tetracenomycin A2 to tetracenomycin C involving two molecules of O(2) and one molecule of H(2)O. | Rafanan ER Jr, Hutchinson CR, Shen B | 2000 Oct 5 | 11009387 |