EC number:1.14.13.50
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.13 With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.13.50 |
|---|---|
| Description: | Pentachlorophenol monooxygenase. |
| Alternative Name: |
Pentachlorophenol hydroxylase. Pentachlorophenol dehalogenase. Pentachlorophenol dechlorinase. Pentachlorophenol 4-monooxygenase. PCP hydroxylase. PCB4MO. PCB 4-monooxygenase. |
| Cath: | 3.30.70.2450; 3.30.9.50; 3.50.50.60; 3.40.30.120; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.13.50 |
| BRENDA Enzyme Link: | BRENDA 1.14.13.50 |
| KEGG Enzyme Link: | KEGG1.14.13.50 |
| BioCyc Enzyme Link: | BioCyc 1.14.13.50 |
| ExPASy Enzyme Link: | ExPASy1.14.13.50 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.13.50 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.13.50 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.13.50 |
| IntEnz Enzyme Link: | IntEnz 1.14.13.50 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.13.50 |
EC number:1.14.13.50
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:18685 | H(+) + NADPH + O2 + pentachlorophenol = 2,3,5,6-tetrachloro-1,4-benzoquinone + chloride + H2O + NADP(+) |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]-[c;H0;+0:10]1:[c;H0;+0:11](-[Cl;H0;+0:12]):[c;H0;+0:13](-[*:14]):[c;H0;+0:15](-[*:16]):[c;H0;+0:17](-[OH;+0:18]):[c;H0;+0:19]:1-[*:20].[O;H0;+0:21]=[O;H0;+0:22]>>[*:9]-[C;H0;+0:10]1=[C;H0;+0:19](-[*:20])-[C;H0;+0:17](=[O;H0;+0:18])-[C;H0;+0:15](-[*:16])=[C;H0;+0:13](-[*:14])-[C;H0;+0:11]-1=[O;H0;+0:22].[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[Cl-;H0:12].[OH2;+0:21] |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| A radical intermediate in the conversion of pentachlorophenol to tetrachlorohydroquinone by Sphingobium chlorophenolicum. | Rudolph J, Erbse AH, Behlen LS, Copley SD | 2014 Oct 21 | 25238136 |
| Pentachlorophenol hydroxylase, a poorly functioning enzyme required for degradation of pentachlorophenol by Sphingobium chlorophenolicum. | Hlouchova K, Rudolph J, Pietari JM, Behlen LS, Copley SD | 2012 May 8 | 22482720 |
| Biochemical characterization of the tetrachlorobenzoquinone reductase involved in the biodegradation of pentachlorophenol. | Chen L, Yang J | 2008 Mar | 19325743 |
| Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723. | Lange CC, Schneider BJ, Orser CS | 1996 Feb 6 | 8619798 |
| Mechanism of enzymatic dehalogenation of pentachlorophenol by Arthrobacter sp. strain ATCC 33790. | Schenk T, Müller R, Lingens F | 1990 Dec | 2254286 |
| Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries. | Xun L, Topp E, Orser CS | 1992 May | 1569020 |
| Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase. | Nakamura T, Motoyama T, Hirono S, Yamaguchi I | 2004 Aug 2 | 15262224 |
| Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase. | Xun L, Topp E, Orser CS | 1992 Sep | 1512208 |
| A previously unrecognized step in pentachlorophenol degradation in Sphingobium chlorophenolicum is catalyzed by tetrachlorobenzoquinone reductase (PcpD). | Dai M, Rogers JB, Warner JR, Copley SD | 2003 Jan | 12486067 |
| RHEA:11440 | 2,3,5,6-tetrachlorophenol + NADPH + O2 = 2,3,5,6-tetrachlorohydroquinone + H2O + NADP(+) |
| RULE(radius=1) | [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[cH;+0:10]:[*:11].[O;H0;+0:12]=[O;H0;+0:13]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[c;H0;+0:10](:[*:11])-[OH;+0:12].[OH2;+0:13] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Verification of the role of PCP 4-monooxygenase in chlorine elimination from pentachlorophenol by Flavobacterium sp. strain ATCC 39723. | Lange CC, Schneider BJ, Orser CS | 1996 Feb 6 | 8619798 |
| Mechanism of enzymatic dehalogenation of pentachlorophenol by Arthrobacter sp. strain ATCC 33790. | Schenk T, Müller R, Lingens F | 1990 Dec | 2254286 |
| Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries. | Xun L, Topp E, Orser CS | 1992 May | 1569020 |
| Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase. | Nakamura T, Motoyama T, Hirono S, Yamaguchi I | 2004 Aug 2 | 15262224 |
| Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase. | Xun L, Topp E, Orser CS | 1992 Sep | 1512208 |
| A previously unrecognized step in pentachlorophenol degradation in Sphingobium chlorophenolicum is catalyzed by tetrachlorobenzoquinone reductase (PcpD). | Dai M, Rogers JB, Warner JR, Copley SD | 2003 Jan | 12486067 |