EC number:1.14.14.29
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.14.29 | ||
|---|---|---|---|
| Description: | 25/26-hydroxycholesterol 7-alpha-hydroxylase. | ||
| Alternative Name: |
27-hydroxycholesterol 7-monooxygenase. 27-hydroxycholesterol 7-alpha-hydroxylase. 25-hydroxycholesterol 7-alpha-monooxygenase. 25-hydroxycholesterol 7-alpha-hydroxylase. | ||
| Prosite: | PDOC00081; | ||
| PDB: |
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3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.14.29 |
| BRENDA Enzyme Link: | BRENDA 1.14.14.29 |
| KEGG Enzyme Link: | KEGG1.14.14.29 |
| BioCyc Enzyme Link: | BioCyc 1.14.14.29 |
| ExPASy Enzyme Link: | ExPASy1.14.14.29 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.14.29 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.14.29 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.14.29 |
| IntEnz Enzyme Link: | IntEnz 1.14.14.29 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.14.29 |
EC number:1.14.14.29
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:24308 | 25-hydroxycholesterol + O2 + reduced [NADPH--hemoprotein reductase] = 7alpha,25-dihydroxycholesterol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[*:3].[*:4]-[N;H0;+0:5]1-[*:6]:[*:7]-[NH;+0:8]-[c;H0;+0:9](:[*:10]):[c;H0;+0:11]-1:[nH;+0:12]:[*:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:14].[*:4]-[n;H0;+0:5]1:[*:6]:[*:7]:[n;H0;+0:8]:[c;H0;+0:9](:[*:10])-[c;H0;+0:11]:1:[n;H0;+0:12]:[*:13].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol. | Li-Hawkins J, Lund EG, Bronson AD, Russell DW | 2000 Jun 2 | 10748047 |
| Identification and characterization of a mouse oxysterol 7alpha-hydroxylase cDNA. | Schwarz M, Lund EG, Lathe R, Björkhem I, Russell DW | 1997 Sep 19 | 9295351 |
| 7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase. | Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Björkhem I | 1994 Sep 1 | 7925343 |
| Human steroid and oxysterol 7α-hydroxylase CYP7B1: substrate specificity, azole binding and misfolding of clinically relevant mutants. | Yantsevich AV, Dichenko YV, Mackenzie F, Mukha DV, Baranovsky AV, Gilep AA, Usanov SA, Strushkevich NV | 2014 Mar | 24491228 |
| Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat. | Ren S, Marques D, Redford K, Hylemon PB, Gil G, Vlahcevic ZR, Pandak WM | 2003 May | 12759897 |
| Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1. | Wu Z, Martin KO, Javitt NB, Chiang JY | 1999 Dec | 10588945 |
| The enzymes, regulation, and genetics of bile acid synthesis. | Russell DW | 2003 | 12543708 |
| Molecular genetics of 3beta-hydroxy-Delta5-C27-steroid oxidoreductase deficiency in 16 patients with loss of bile acid synthesis and liver disease. | Cheng JB, Jacquemin E, Gerhardt M, Nazer H, Cresteil D, Heubi JE, Setchell KD, Russell DW | 2003 Apr | 12679481 |
| RHEA:19041 | (25R)-cholest-5-ene-3beta,26-diol + O2 + reduced [NADPH--hemoprotein reductase] = (25R)-cholest-5-en-3beta,7alpha,26-triol + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[*:3].[*:4]-[N;H0;+0:5]1-[*:6]:[*:7]-[NH;+0:8]-[c;H0;+0:9](:[*:10]):[c;H0;+0:11]-1:[nH;+0:12]:[*:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:14].[*:4]-[n;H0;+0:5]1:[*:6]:[*:7]:[n;H0;+0:8]:[c;H0;+0:9](:[*:10])-[c;H0;+0:11]:1:[n;H0;+0:12]:[*:13].[OH2;+0:15] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol. | Li-Hawkins J, Lund EG, Bronson AD, Russell DW | 2000 Jun 2 | 10748047 |
| Identification and characterization of a mouse oxysterol 7alpha-hydroxylase cDNA. | Schwarz M, Lund EG, Lathe R, Björkhem I, Russell DW | 1997 Sep 19 | 9295351 |
| 7 alpha hydroxylation of 25-hydroxycholesterol in liver microsomes. Evidence that the enzyme involved is different from cholesterol 7 alpha-hydroxylase. | Toll A, Wikvall K, Sudjana-Sugiaman E, Kondo KH, Björkhem I | 1994 Sep 1 | 7925343 |
| Human steroid and oxysterol 7α-hydroxylase CYP7B1: substrate specificity, azole binding and misfolding of clinically relevant mutants. | Yantsevich AV, Dichenko YV, Mackenzie F, Mukha DV, Baranovsky AV, Gilep AA, Usanov SA, Strushkevich NV | 2014 Mar | 24491228 |
| Regulation of oxysterol 7alpha-hydroxylase (CYP7B1) in the rat. | Ren S, Marques D, Redford K, Hylemon PB, Gil G, Vlahcevic ZR, Pandak WM | 2003 May | 12759897 |
| Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1. | Wu Z, Martin KO, Javitt NB, Chiang JY | 1999 Dec | 10588945 |
| The enzymes, regulation, and genetics of bile acid synthesis. | Russell DW | 2003 | 12543708 |