EC number:1.14.14.48
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.14.48 |
|---|---|
| Description: | Jasmonoyl-L-amino acid 12-hydroxylase. |
| Alternative Name: |
CYP94B3. CYP94B1. |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.14.48 |
| BRENDA Enzyme Link: | BRENDA 1.14.14.48 |
| KEGG Enzyme Link: | KEGG1.14.14.48 |
| BioCyc Enzyme Link: | BioCyc 1.14.14.48 |
| ExPASy Enzyme Link: | ExPASy1.14.14.48 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.14.48 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.14.48 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.14.48 |
| IntEnz Enzyme Link: | IntEnz 1.14.14.48 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.14.48 |
EC number:1.14.14.48
| MSA: | |
|---|---|
| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:54832 | a jasmonyl-L-amino acid + O2 + reduced [NADPH--hemoprotein reductase] = a 12-hydroxyjasmonyl-L-alpha-amino acid + H(+) + H2O + oxidized [NADPH--hemoprotein reductase] |
| RULE(radius=1) | [*:1]-[CH3;+0:2].[*:3]-[N;H0;+0:4]1-[*:5]:[*:6]-[NH;+0:7]-[c;H0;+0:8](:[*:9]):[c;H0;+0:10]-1:[nH;+0:11]:[*:12].[O;H0;+0:13]=[O;H0;+0:14]>>[*:1]-[CH2;+0:2]-[OH;+0:13].[*:3]-[n;H0;+0:4]1:[*:5]:[*:6]:[n;H0;+0:7]:[c;H0;+0:8](:[*:9])-[c;H0;+0:10]:1:[n;H0;+0:11]:[*:12].[OH2;+0:14] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates. | Widemann E, Grausem B, Renault H, Pineau E, Heinrich C, Lugan R, Ullmann P, Miesch L, Aubert Y, Miesch M, Heitz T, Pinot F | 2015 Sep | 26164240 |
| Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis. | Koo AJ, Thireault C, Zemelis S, Poudel AN, Zhang T, Kitaoka N, Brandizzi F, Matsuura H, Howe GA | 2014 Oct 24 | 25210037 |
| CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite. | Kitaoka N, Kawaide H, Amano N, Matsubara T, Nabeta K, Takahashi K, Matsuura H | 2014 Mar | 24467969 |
| Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover. | Heitz T, Widemann E, Lugan R, Miesch L, Ullmann P, Désaubry L, Holder E, Grausem B, Kandel S, Miesch M, Werck-Reichhart D, Pinot F | 2012 Feb 24 | 22215670 |
| Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate. | Kitaoka N, Matsubara T, Sato M, Takahashi K, Wakuta S, Kawaide H, Matsui H, Nabeta K, Matsuura H | 2011 Oct | 21849397 |
| Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine. | Koo AJ, Cooke TF, Howe GA | 2011 May 31 | 21576464 |