EC number:1.14.14.90

Enzyme

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     1. Oxidoreductases
        1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
            1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
ID:1.14.14.90
Description:Isoflavone 2'-hydroxylase.
Alternative Name: Isoflavone 2'-monooxygenase.
CYP81E1.
CYP Ge-3.
Prosite: PDOC00081;
PDB:
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3D structure

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PDB

References

External Links

UniProtKB Enzyme Link: UniProtKB 1.14.14.90
BRENDA Enzyme Link: BRENDA 1.14.14.90
KEGG Enzyme Link: KEGG1.14.14.90
BioCyc Enzyme Link: BioCyc 1.14.14.90
ExPASy Enzyme Link: ExPASy1.14.14.90
EC2PDB Enzyme Link: EC2PDB 1.14.14.90
ExplorEnz Enzyme Link: ExplorEnz 1.14.14.90
PRIAM enzyme-specific profiles Link: PRIAM 1.14.14.90
IntEnz Enzyme Link: IntEnz 1.14.14.90
MEDLINE Enzyme Link: MEDLINE 1.14.14.90

EC number:1.14.14.90

MSA:

1.14.14.90;
Phylogenetic Tree:

1.14.14.90;
Uniprot:
M-CSA:
RHEA:56108 genistein + O2 + reduced [NADPH--hemoprotein reductase] = 2'-hydroxygenistein + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]
RULE(radius=1) [*:1]-[N;H0;+0:2]1-[*:3]:[*:4]-[NH;+0:5]-[c;H0;+0:6](:[*:7]):[c;H0;+0:8]-1:[nH;+0:9]:[*:10].[*:11]:[cH;+0:12]:[*:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n;H0;+0:2]1:[*:3]:[*:4]:[n;H0;+0:5]:[c;H0;+0:6](:[*:7])-[c;H0;+0:8]:1:[n;H0;+0:9]:[*:10].[*:11]:[c;H0;+0:12](:[*:13])-[OH;+0:14].[OH2;+0:15]
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References

TitleAuthorsDatePubMed ID
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.Akashi T, Aoki T, Ayabe S1998 Oct 99790908
RHEA:53208 daidzein + O2 + reduced [NADPH--hemoprotein reductase] = 2'-hydroxydaidzein + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]
RULE(radius=1) [*:1]-[N;H0;+0:2]1-[*:3]:[*:4]-[NH;+0:5]-[c;H0;+0:6](:[*:7]):[c;H0;+0:8]-1:[nH;+0:9]:[*:10].[*:11]:[cH;+0:12]:[*:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n;H0;+0:2]1:[*:3]:[*:4]:[n;H0;+0:5]:[c;H0;+0:6](:[*:7])-[c;H0;+0:8]:1:[n;H0;+0:9]:[*:10].[*:11]:[c;H0;+0:12](:[*:13])-[OH;+0:14].[OH2;+0:15]
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References

TitleAuthorsDatePubMed ID
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.Akashi T, Aoki T, Ayabe S1998 Oct 99790908
RHEA:18849 a 2'-unsubstituted isoflavone + O2 + reduced [NADPH--hemoprotein reductase] = a 2'-hydroxyisoflavone + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]
RULE(radius=1) [*:1]-[N;H0;+0:2]1-[*:3]:[*:4]-[NH;+0:5]-[c;H0;+0:6](:[*:7]):[c;H0;+0:8]-1:[nH;+0:9]:[*:10].[*:11]:[cH;+0:12]:[*:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[n;H0;+0:2]1:[*:3]:[*:4]:[n;H0;+0:5]:[c;H0;+0:6](:[*:7])-[c;H0;+0:8]:1:[n;H0;+0:9]:[*:10].[*:11]:[c;H0;+0:12](:[*:13])-[OH;+0:14].[OH2;+0:15]
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References

TitleAuthorsDatePubMed ID
CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase.Akashi T, Aoki T, Ayabe S1998 Oct 99790908
RHEA:12388 formononetin + O2 + reduced [NADPH--hemoprotein reductase] = 2'-hydroxyformononetin + H(+) + H2O + oxidized [NADPH--hemoprotein reductase]
RULE(radius=1) [*:1]-[N;H0;+0:2]1-[CH;+0:3]=[C;H0;+0:4](-[*:5])-[CH2;+0:6]-[CH;+0:7]=[CH;+0:8]-1.[*:9]:[cH;+0:10]:[*:11].[H+;H0:12].[O;H0;+0:13]=[O;H0;+0:14]>>[*:1]-[n+;H0:2]1:[cH;+0:3]:[c;H0;+0:4](-[*:5]):[cH;+0:6]:[cH;+0:7]:[cH;+0:8]:1.[*:9]:[c;H0;+0:10](:[*:11])-[OH;+0:13].[OH2;+0:14]
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