EC number:1.14.15.18
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen |
| 1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor |
| ID: | 1.14.15.18 | ||
|---|---|---|---|
| Description: | Calcidiol 1-monooxygenase. | ||
| Alternative Name: |
25-OHD-1 alpha-hydroxylase. 25-hydroxyvitamin D-1 alpha hydroxylase. 25-hydroxyvitamin D(3) 1-alpha-hydroxylase. 25-hydroxycholecalciferol 1-monooxygenase. 25-hydroxycholecalciferol 1-hydroxylase. | ||
| Prosite: | PDOC00081; | ||
| PDB: |
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| Cath: | 1.10.630.10; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.14.15.18 |
| BRENDA Enzyme Link: | BRENDA 1.14.15.18 |
| KEGG Enzyme Link: | KEGG1.14.15.18 |
| BioCyc Enzyme Link: | BioCyc 1.14.15.18 |
| ExPASy Enzyme Link: | ExPASy1.14.15.18 |
| EC2PDB Enzyme Link: | EC2PDB 1.14.15.18 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.14.15.18 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.14.15.18 |
| IntEnz Enzyme Link: | IntEnz 1.14.15.18 |
| MEDLINE Enzyme Link: | MEDLINE 1.14.15.18 |
EC number:1.14.15.18
| MSA: | |
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| Phylogenetic Tree: | |
| Uniprot: | |
| M-CSA: |
| RHEA:49064 | 2 H(+) + O2 + 2 reduced [adrenodoxin] + secalciferol = calcitetrol + H2O + 2 oxidized [adrenodoxin] |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[*:3].[*:4]-[Fe;H0;+0:5]-[*:6].[*:7]-[Fe;H0;+0:8]-[*:9].[H+;H0:10].[H+;H0:11].[O;H0;+0:12]=[O;H0;+0:13]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:12].[*:4]-[Fe+;H0:5]-[*:6].[*:7]-[Fe+;H0:8]-[*:9].[OH2;+0:13] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Expression of human CYP27B1 in Escherichia coli and characterization in phospholipid vesicles. | Tang EKY, Tieu EW, Tuckey RC | 2012 Oct | 22862690 |
| Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli. | Sawada N, Sakaki T, Kitanaka S, Takeyama K, Kato S, Inouye K | 1999 Nov | 10518789 |
| Enzymatic properties of mouse 25-hydroxyvitamin D3 1 alpha-hydroxylase expressed in Escherichia coli. | Sakaki T, Sawada N, Takeyama K, Kato S, Inouye K | 1999 Feb | 10092858 |
| RHEA:20573 | calcidiol + 2 H(+) + O2 + 2 reduced [adrenodoxin] = calcitriol + H2O + 2 oxidized [adrenodoxin] |
| RULE(radius=1) | [*:1]-[CH2;+0:2]-[*:3].[*:4]-[Fe;H0;+0:5]-[*:6].[*:7]-[Fe;H0;+0:8]-[*:9].[H+;H0:10].[H+;H0:11].[O;H0;+0:12]=[O;H0;+0:13]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:12].[*:4]-[Fe+;H0:5]-[*:6].[*:7]-[Fe+;H0:8]-[*:9].[OH2;+0:13] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Inactivating mutations in the 25-hydroxyvitamin D3 1alpha-hydroxylase gene in patients with pseudovitamin D-deficiency rickets. | Kitanaka S, Takeyama K, Murayama A, Sato T, Okumura K, Nogami M, Hasegawa Y, Niimi H, Yanagisawa J, Tanaka T, Kato S | 1998 Mar 5 | 9486994 |
| 25-Hydroxycholecalciferol-1-hydroxylase. Subcellular location and properties. | Gray RW, Omdahl JL, Ghazarian JG, DeLuca HF | 1972 Dec 10 | 4404596 |
| Expression of human CYP27B1 in Escherichia coli and characterization in phospholipid vesicles. | Tang EKY, Tieu EW, Tuckey RC | 2012 Oct | 22862690 |
| Identification of the amino acid residue of CYP27B1 responsible for binding of 25-hydroxyvitamin D3 whose mutation causes vitamin D-dependent rickets type 1. | Yamamoto K, Uchida E, Urushino N, Sakaki T, Kagawa N, Sawada N, Kamakura M, Kato S, Inouye K, Yamada S | 2005 Aug 26 | 15972816 |
| Metabolism of vitamin D by human microsomal CYP2R1. | Shinkyo R, Sakaki T, Kamakura M, Ohta M, Inouye K | 2004 Nov 5 | 15465040 |
| Regulation of 25-hydroxyvitamin D3-1 alpha-hydroxylase and production of 1 alpha,25-dihydroxyvitamin D3 by human dendritic cells. | Fritsche J, Mondal K, Ehrnsperger A, Andreesen R, Kreutz M | 2003 Nov 1 | 12855575 |
| Synthesis of 1,25-dihydroxyvitamin D(3) by human endothelial cells is regulated by inflammatory cytokines: a novel autocrine determinant of vascular cell adhesion. | Zehnder D, Bland R, Chana RS, Wheeler DC, Howie AJ, Williams MC, Stewart PM, Hewison M | 2002 Mar | 11856765 |
| Metabolism of vitamin D(3) by human CYP27A1. | Sawada N, Sakaki T, Ohta M, Inouye K | 2000 Jul 14 | 10891358 |