EC number:1.14.15.33

Enzyme

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     1. Oxidoreductases
        1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
            1.14.15 With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
ID:1.14.15.33
Description:Pikromycin synthase.
Alternative Name: CYP107L1.

3D structure

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PDB

References

External Links

UniProtKB Enzyme Link: UniProtKB 1.14.15.33
BRENDA Enzyme Link: BRENDA 1.14.15.33
KEGG Enzyme Link: KEGG1.14.15.33
BioCyc Enzyme Link: BioCyc 1.14.15.33
ExPASy Enzyme Link: ExPASy1.14.15.33
EC2PDB Enzyme Link: EC2PDB 1.14.15.33
ExplorEnz Enzyme Link: ExplorEnz 1.14.15.33
PRIAM enzyme-specific profiles Link: PRIAM 1.14.15.33
IntEnz Enzyme Link: IntEnz 1.14.15.33
MEDLINE Enzyme Link: MEDLINE 1.14.15.33

EC number:1.14.15.33

MSA:

1.14.15.33;
Phylogenetic Tree:

1.14.15.33;
Uniprot:
M-CSA:
RHEA:39887 2 H(+) + narbomycin + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = H2O + 2 oxidized [2Fe-2S]-[ferredoxin] + pikromycin
RULE(radius=1) [*:1]-[CH;+0:2](-[*:3])-[*:4].[*:5]-[N;H0;+0:6]1-[CH;+0:7]=[C;H0;+0:8](-[*:9])-[CH2;+0:10]-[CH;+0:11]=[CH;+0:12]-1.[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[OH;+0:14].[*:5]-[n+;H0:6]1:[cH;+0:7]:[c;H0;+0:8](-[*:9]):[cH;+0:10]:[cH;+0:11]:[cH;+0:12]:1.[OH2;+0:15]
Reaction
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References

TitleAuthorsDatePubMed ID
Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae.Xue Y, Wilson D, Zhao L, Liu Hw, Sherman DH1998 Nov9831532
Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae.Li S, Ouellet H, Sherman DH, Podust LM2009 Feb 2719124459
The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae.Sherman DH, Li S, Yermalitskaya LV, Kim Y, Smith JA, Waterman MR, Podust LM2006 Sep 816825192
RHEA:40531 2 H(+) + narbomycin + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = H2O + neopikromycin + 2 oxidized [2Fe-2S]-[ferredoxin]
RULE(radius=1) [*:1]-[CH2;+0:2]-[*:3].[*:4]-[N;H0;+0:5]1-[CH;+0:6]=[C;H0;+0:7](-[*:8])-[CH2;+0:9]-[CH;+0:10]=[CH;+0:11]-1.[H+;H0:12].[O;H0;+0:13]=[O;H0;+0:14]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:13].[*:4]-[n+;H0:5]1:[cH;+0:6]:[c;H0;+0:7](-[*:8]):[cH;+0:9]:[cH;+0:10]:[cH;+0:11]:1.[OH2;+0:14]
Reaction
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae.Xue Y, Wilson D, Zhao L, Liu Hw, Sherman DH1998 Nov9831532
Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae.Li S, Ouellet H, Sherman DH, Podust LM2009 Feb 2719124459
The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae.Sherman DH, Li S, Yermalitskaya LV, Kim Y, Smith JA, Waterman MR, Podust LM2006 Sep 816825192
Neopikromycin and novapikromycin from the pikromycin biosynthetic pathway of Streptomyces venezuelae.Lee SK, Park JW, Kim JW, Jung WS, Park SR, Choi CY, Kim ES, Kim BS, Ahn JS, Sherman DH, Yoon YJ2006 May16724858
RHEA:40535 4 H(+) + narbomycin + 2 O2 + 4 reduced [2Fe-2S]-[ferredoxin] = 2 H2O + novapikromycin + 4 oxidized [2Fe-2S]-[ferredoxin]
RULE(radius=1) [*:1]-[CH2;+0:2]-[*:3]-[CH;+0:4](-[*:5])-[*:6].[*:7]-[N;H0;+0:8]1-[CH;+0:9]=[C;H0;+0:10](-[*:11])-[CH2;+0:12]-[CH;+0:13]=[CH;+0:14]-1.[H+;H0:15].[H+;H0:16].[O;H0;+0:17]=[O;H0;+0:18].[O;H0;+0:19]=[O;H0;+0:20]>>[*:1]-[CH;+0:2](-[OH;+0:17])-[*:3]-[C;H0;+0:4](-[*:5])(-[*:6])-[OH;+0:18].[*:7]-[n+;H0:8]1:[cH;+0:9]:[c;H0;+0:10](-[*:11]):[cH;+0:12]:[cH;+0:13]:[cH;+0:14]:1.[OH2;+0:19].[OH2;+0:20]
Reaction
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae.Xue Y, Wilson D, Zhao L, Liu Hw, Sherman DH1998 Nov9831532
Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae.Li S, Ouellet H, Sherman DH, Podust LM2009 Feb 2719124459
The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae.Sherman DH, Li S, Yermalitskaya LV, Kim Y, Smith JA, Waterman MR, Podust LM2006 Sep 816825192
Neopikromycin and novapikromycin from the pikromycin biosynthetic pathway of Streptomyces venezuelae.Lee SK, Park JW, Kim JW, Jung WS, Park SR, Choi CY, Kim ES, Kim BS, Ahn JS, Sherman DH, Yoon YJ2006 May16724858
RHEA:40539 10-deoxymethymycin + 2 H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = H2O + methymycin + 2 oxidized [2Fe-2S]-[ferredoxin]
RULE(radius=1) [*:1]-[CH;+0:2](-[*:3])-[*:4].[*:5]-[N;H0;+0:6]1-[CH;+0:7]=[C;H0;+0:8](-[*:9])-[CH2;+0:10]-[CH;+0:11]=[CH;+0:12]-1.[H+;H0:13].[O;H0;+0:14]=[O;H0;+0:15]>>[*:1]-[C;H0;+0:2](-[*:3])(-[*:4])-[OH;+0:14].[*:5]-[n+;H0:6]1:[cH;+0:7]:[c;H0;+0:8](-[*:9]):[cH;+0:10]:[cH;+0:11]:[cH;+0:12]:1.[OH2;+0:15]
Reaction
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae.Xue Y, Wilson D, Zhao L, Liu Hw, Sherman DH1998 Nov9831532
Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae.Li S, Ouellet H, Sherman DH, Podust LM2009 Feb 2719124459
The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae.Sherman DH, Li S, Yermalitskaya LV, Kim Y, Smith JA, Waterman MR, Podust LM2006 Sep 816825192
RHEA:40543 10-deoxymethymycin + 2 H(+) + O2 + 2 reduced [2Fe-2S]-[ferredoxin] = H2O + neomethymycin + 2 oxidized [2Fe-2S]-[ferredoxin]
RULE(radius=1) [*:1]-[CH2;+0:2]-[*:3].[*:4]-[N;H0;+0:5]1-[CH;+0:6]=[C;H0;+0:7](-[*:8])-[CH2;+0:9]-[CH;+0:10]=[CH;+0:11]-1.[H+;H0:12].[O;H0;+0:13]=[O;H0;+0:14]>>[*:1]-[CH;+0:2](-[*:3])-[OH;+0:13].[*:4]-[n+;H0:5]1:[cH;+0:6]:[c;H0;+0:7](-[*:8]):[cH;+0:9]:[cH;+0:10]:[cH;+0:11]:1.[OH2;+0:14]
Reaction
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae.Xue Y, Wilson D, Zhao L, Liu Hw, Sherman DH1998 Nov9831532
Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae.Li S, Ouellet H, Sherman DH, Podust LM2009 Feb 2719124459
The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae.Sherman DH, Li S, Yermalitskaya LV, Kim Y, Smith JA, Waterman MR, Podust LM2006 Sep 816825192
Isolation and structure determination of novamethymycin, a new bioactive metabolite of the methymycin biosynthetic pathway in Streptomyces venezuelae.Zhang Q, Sherman DH2001 Nov11720530
RHEA:40547 10-deoxymethymycin + 4 H(+) + 2 O2 + 4 reduced [2Fe-2S]-[ferredoxin] = 2 H2O + novamethymycin + 4 oxidized [2Fe-2S]-[ferredoxin]
RULE(radius=1) [*:1]-[CH2;+0:2]-[*:3]-[CH;+0:4](-[*:5])-[*:6].[*:7]-[N;H0;+0:8]1-[CH;+0:9]=[C;H0;+0:10](-[*:11])-[CH2;+0:12]-[CH;+0:13]=[CH;+0:14]-1.[H+;H0:15].[H+;H0:16].[O;H0;+0:17]=[O;H0;+0:18].[O;H0;+0:19]=[O;H0;+0:20]>>[*:1]-[CH;+0:2](-[OH;+0:17])-[*:3]-[C;H0;+0:4](-[*:5])(-[*:6])-[OH;+0:18].[*:7]-[n+;H0:8]1:[cH;+0:9]:[c;H0;+0:10](-[*:11]):[cH;+0:12]:[cH;+0:13]:[cH;+0:14]:1.[OH2;+0:19].[OH2;+0:20]
Reaction
Core-to-Core More

References

TitleAuthorsDatePubMed ID
Hydroxylation of macrolactones YC-17 and narbomycin is mediated by the pikC-encoded cytochrome P450 in Streptomyces venezuelae.Xue Y, Wilson D, Zhao L, Liu Hw, Sherman DH1998 Nov9831532
Analysis of transient and catalytic desosamine-binding pockets in cytochrome P-450 PikC from Streptomyces venezuelae.Li S, Ouellet H, Sherman DH, Podust LM2009 Feb 2719124459
The structural basis for substrate anchoring, active site selectivity, and product formation by P450 PikC from Streptomyces venezuelae.Sherman DH, Li S, Yermalitskaya LV, Kim Y, Smith JA, Waterman MR, Podust LM2006 Sep 816825192
Isolation and structure determination of novamethymycin, a new bioactive metabolite of the methymycin biosynthetic pathway in Streptomyces venezuelae.Zhang Q, Sherman DH2001 Nov11720530