EC number:1.4.1.3
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.4 Acting on the CH-NH2 group of donors |
| 1.4.1 With NAD+ or NADP+ as acceptor |
| ID: | 1.4.1.3 | ||
|---|---|---|---|
| Description: | Glutamate dehydrogenase (NAD(P)(+)). | ||
| Alternative Name: |
Glutamic dehydrogenase. | ||
| Prosite: | PDOC00071; | ||
| PDB: |
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| Cath: | 1.10.287.140; 3.40.50.720; 3.40.50.10860; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.4.1.3 |
| BRENDA Enzyme Link: | BRENDA 1.4.1.3 |
| KEGG Enzyme Link: | KEGG1.4.1.3 |
| BioCyc Enzyme Link: | BioCyc 1.4.1.3 |
| ExPASy Enzyme Link: | ExPASy1.4.1.3 |
| EC2PDB Enzyme Link: | EC2PDB 1.4.1.3 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.4.1.3 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.4.1.3 |
| IntEnz Enzyme Link: | IntEnz 1.4.1.3 |
| MEDLINE Enzyme Link: | MEDLINE 1.4.1.3 |
| RHEA:15133 | H2O + L-glutamate + NAD(+) = 2-oxoglutarate + H(+) + NADH + NH4(+) |
| RULE(radius=1) | [*:1]-[CH;+0:2](-[*:3])-[NH2;+0:4].[*:5]-[n+;H0:6]1:[cH;+0:7]:[cH;+0:8]:[cH;+0:9]:[c;H0;+0:10](-[*:11]):[cH;+0:12]:1.[OH2;+0:13]>>[*:1]-[C;H0;+0:2](-[*:3])=[O;H0;+0:13].[*:5]-[N;H0;+0:6]1-[CH;+0:7]=[CH;+0:8]-[CH2;+0:9]-[C;H0;+0:10](-[*:11])=[CH;+0:12]-1.[NH3;+0:4] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| The catalytic role of aspartate in the active site of glutamate dehydrogenase. | Dean JL, Wang XG, Teller JK, Waugh ML, Britton KL, Baker PJ, Stillman TJ, Martin SR, Rice DW, Engel PC | 1994 Jul 1 | 8037659 |
| Biochemical characterization of two glutamate dehydrogenases with different cofactor specificities from a hyperthermophilic archaeon Pyrobaculum calidifontis. | Wakamatsu T, Higashi C, Ohmori T, Doi K, Ohshima T | 2013 May | 23508687 |
| Functional dissection of a trigger enzyme: mutations of the bacillus subtilis glutamate dehydrogenase RocG that affect differentially its catalytic activity and regulatory properties. | Gunka K, Newman JA, Commichau FM, Herzberg C, Rodrigues C, Hewitt L, Lewis RJ, Stülke J | 2010 Jul 23 | 20630473 |
| Probing the determinants of coenzyme specificity in Peptostreptococcus asaccharolyticus glutamate dehydrogenase by site-directed mutagenesis. | Carrigan JB, Engel PC | 2007 Oct | 17850332 |
| Molecular properties and enhancement of thermostability by random mutagenesis of glutamate dehydrogenase from Bacillus subtilis. | Khan MI, Ito K, Kim H, Ashida H, Ishikawa T, Shibata H, Sawa Y | 2005 Oct | 16244435 |
| Properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe Peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an Escherichia coli host. | Carrigan JB, Coughlan S, Engel PC | 2005 Mar 1 | 15727821 |
| Molecular gene cloning, expression, and characterization of bovine brain glutamate dehydrogenase. | Kim DW, Eum WS, Jang SH, Yoon CS, Kim YH, Choi SH, Choi HS, Kim SY, Kwon HY, Kang JH, Kwon OS, Cho SW, Park J, Choi SY | 2003 Nov 30 | 14659072 |
| The gdhB gene of Pseudomonas aeruginosa encodes an arginine-inducible NAD(+)-dependent glutamate dehydrogenase which is subject to allosteric regulation. | Lu CD, Abdelal AT | 2001 Jan | 11133942 |
| RHEA:11612 | H2O + L-glutamate + NADP(+) = 2-oxoglutarate + H(+) + NADPH + NH4(+) |
| RULE(radius=1) | [*:1]-[CH;+0:2](-[*:3])-[NH2;+0:4].[*:5]-[n+;H0:6]1:[cH;+0:7]:[cH;+0:8]:[cH;+0:9]:[c;H0;+0:10](-[*:11]):[cH;+0:12]:1.[OH2;+0:13]>>[*:1]-[C;H0;+0:2](-[*:3])=[O;H0;+0:13].[*:5]-[N;H0;+0:6]1-[CH;+0:7]=[CH;+0:8]-[CH2;+0:9]-[C;H0;+0:10](-[*:11])=[CH;+0:12]-1.[NH3;+0:4] |
| Reaction |  |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. | Tempest DW, Meers JL, Brown CM | 1970 Apr | 5420057 |
| Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein. | Miller RE, Stadtman ER | 1972 Nov 25 | 4565085 |
| Determination of the midpoint potential of the FAD and FMN flavin cofactors and of the 3Fe-4S cluster of glutamate synthase. | Ravasio S, Curti B, Vanoni MA | 2001 May 8 | 11331018 |
| Glutamate synthase: a complex iron-sulfur flavoprotein. | Vanoni MA, Curti B | 1999 Apr | 10357231 |