EC number:1.5.1.20
Enzyme
Download| EC Tree |
| 1. Oxidoreductases |
| 1.5 Acting on the CH-NH group of donors |
| 1.5.1 With NAD+ or NADP+ as acceptor |
| ID: | 1.5.1.20 |
|---|---|
| Description: | Methylenetetrahydrofolate reductase (NAD(P)H). |
| Alternative Name: |
N(5,10)-methylenetetrahydrofolate reductase. N(5),N(10)-methylenetetrahydrofolate reductase. MTHFR. Methylenetetrahydrofolic acid reductase. Methylenetetrahydrofolate reductase (NADPH). Methylenetetrahydrofolate reductase (NADPH(2)). Methylenetetrahydrofolate reductase. reductase. Methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) phosphate) reductase. Methylenetetrahydrofolate (reduced nicotinamide adenine dinucleotide 5-methyltetrahydrofolate:NADP(+) oxidoreductase. 5-methyltetrahydrofolate:NAD(+) oxidoreductase. 5-methyltetrahydrofolate:NAD oxidoreductase. 5-methyltetrahydrofolate:(acceptor) oxidoreductase. 5,10-methylenetetrahydropteroylglutamate reductase. 5,10-methylenetetrahydrofolic acid reductase. 5,10-methylenetetrahydrofolate reductase (NADPH). 5,10-methylenetetrahydrofolate reductase (FADH(2)). 5,10-methylenetetrahydrofolate reductase. 5,10-CH(2)-H(4)folate reductase. |
| Cath: | 3.20.20.220; |
3D structure
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External Links
| UniProtKB Enzyme Link: | UniProtKB 1.5.1.20 |
| BRENDA Enzyme Link: | BRENDA 1.5.1.20 |
| KEGG Enzyme Link: | KEGG1.5.1.20 |
| BioCyc Enzyme Link: | BioCyc 1.5.1.20 |
| ExPASy Enzyme Link: | ExPASy1.5.1.20 |
| EC2PDB Enzyme Link: | EC2PDB 1.5.1.20 |
| ExplorEnz Enzyme Link: | ExplorEnz 1.5.1.20 |
| PRIAM enzyme-specific profiles Link: | PRIAM 1.5.1.20 |
| IntEnz Enzyme Link: | IntEnz 1.5.1.20 |
| MEDLINE Enzyme Link: | MEDLINE 1.5.1.20 |
| RHEA:19821 | (6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH |
| RULE(radius=1) | ([*:1]-[CH3;+0:2].[*:3]-[NH;+0:4]-[*:5]).[*:6]-[n+;H0:7]1:[cH;+0:8]:[cH;+0:9]:[cH;+0:10]:[c;H0;+0:11](-[*:12]):[cH;+0:13]:1>>[*:3]-[N;H0;+0:4](-[*:5])-[CH2;+0:2]-[*:1].[*:6]-[N;H0;+0:7]1-[CH;+0:8]=[CH;+0:9]-[CH2;+0:10]-[C;H0;+0:11](-[*:12])=[CH;+0:13]-1 |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. | Sheppard CA, Trimmer EE, Matthews RG | 1999 Feb | 9922232 |
| Purification and properties of methylenetetrahydrofolate reductase from pig liver. | Daubner SC, Matthews RG | 1982 Jan 10 | 6975779 |
| Mammalian methylenetetrahydrofolate reductase. Partial purification, properties, and inhibition by S-adenosylmethionine. | Kutzbach C, Stokstad EL | 1971 Dec 15 | 4399897 |
| Insights into severe 5,10-methylenetetrahydrofolate reductase deficiency: molecular genetic and enzymatic characterization of 76 patients. | Burda P, Schäfer A, Suormala T, Rummel T, Bürer C, Heuberger D, Frapolli M, Giunta C, Sokolová J, Vlášková H, Kožich V, Koch HG, Fowler B, Froese DS, Baumgartner MR | 2015 Jun | 25736335 |
| The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia. | Guenther BD, Sheppard CA, Tran P, Rozen R, Matthews RG, Ludwig ML | 1999 Apr | 10201405 |
| RHEA:19817 | (6S)-5-methyl-5,6,7,8-tetrahydrofolate + NADP(+) = (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + H(+) + NADPH |
| RULE(radius=1) | ([*:1]-[CH3;+0:2].[*:3]-[NH;+0:4]-[*:5]).[*:6]-[n+;H0:7]1:[cH;+0:8]:[cH;+0:9]:[cH;+0:10]:[c;H0;+0:11](-[*:12]):[cH;+0:13]:1>>[*:3]-[N;H0;+0:4](-[*:5])-[CH2;+0:2]-[*:1].[*:6]-[N;H0;+0:7]1-[CH;+0:8]=[CH;+0:9]-[CH2;+0:10]-[C;H0;+0:11](-[*:12])=[CH;+0:13]-1 |
| Reaction |  |
| Core-to-Core | |
| Core-to-Core |
References
| Title | Authors | Date | PubMed ID |
|---|---|---|---|
| Purification and properties of NADH-dependent 5, 10-methylenetetrahydrofolate reductase (MetF) from Escherichia coli. | Sheppard CA, Trimmer EE, Matthews RG | 1999 Feb | 9922232 |
| Purification and properties of methylenetetrahydrofolate reductase from pig liver. | Daubner SC, Matthews RG | 1982 Jan 10 | 6975779 |
| Mammalian methylenetetrahydrofolate reductase. Partial purification, properties, and inhibition by S-adenosylmethionine. | Kutzbach C, Stokstad EL | 1971 Dec 15 | 4399897 |
| Insights into severe 5,10-methylenetetrahydrofolate reductase deficiency: molecular genetic and enzymatic characterization of 76 patients. | Burda P, Schäfer A, Suormala T, Rummel T, Bürer C, Heuberger D, Frapolli M, Giunta C, Sokolová J, Vlášková H, Kožich V, Koch HG, Fowler B, Froese DS, Baumgartner MR | 2015 Jun | 25736335 |
| The structure and properties of methylenetetrahydrofolate reductase from Escherichia coli suggest how folate ameliorates human hyperhomocysteinemia. | Guenther BD, Sheppard CA, Tran P, Rozen R, Matthews RG, Ludwig ML | 1999 Apr | 10201405 |